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Pendampingan Rehabilitasi Terumbu Karang di Pulau Gili Labak dengan Aplikasi Bioreeftek dari Limbah Batok Kelapa (Cocos Nucifera) Qomariyah, Adinda Nuril; Putri, Aprilia; Rahmaniah, Siti; Ning Asih, Eka Nurrahema
Jurnal Ilmiah Pangabdhi Vol 9, No 2: Oktober 2023
Publisher : LPPM Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/pangabdhi.v9i2.20173

Abstract

The high interest of tourists in coral reef snorkeling activities on Gili Labak Island has resulted in considerable damage to the coral reef ecosystem and has the potential to reduce the income of the people of Gili Labak. The purpose of this activity is to provide understanding, outreach, and practice of coral reef rehabilitation using the bioreeftec method from coconut shell waste to tourists and local residents on Gili Labak Island. The realization of this counseling activity was carried out by referring to the root of the problems experienced by the coastal communities of Gili Labak Island, including: 1) The local coastal community has not been educated in preserving the coral reef ecosystem for the continuation of snorkeling tourism activities, 2) The low level of awareness of tourists regarding behavior that threatens the existence of coral reefs during snorkeling activities, 3) There is no concern from the local government regarding the provision of instructions and prohibitions on tourist activities that have the potential to damage coral reefs on the island of Gili Labak. An alternative solution to the problem is conducting counseling activities and coral reef rehabilitation practices on Gili Labak Island using the bioreeftec method from coconut shell waste. The results of coral reef rehabilitation counseling activities on Gili Labak Island showed that as many as 89% of the people of Gili Labak Island were interested in rehabilitating coral reefs to preserve the coral reef ecosystem on the island.
AKTIVITAS ANTIBAKTERI DARI SENYAWA FITOKIMIA EKSTRAK DAUN Avicennia marina DAN Avicennia alba TERHADAP ISOLAT BAKTERI Vibrio sp. DARI TAMBAK UDANG INTENSIF Fittroh, Lisdiana Miftakhul; Ning Asih, Eka Nurrahema; Safitri, Rosita
Jurnal Riset Akuakultur Vol 20, No 2 (2025): Juni (2025)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.20.2.2025.147-164

Abstract

Bakteri Vibrio sp. merupakan kelompok bakteri patogen yang dapat menyebabkan penyakit vibriosis, sehingga memicu tingginya potensi kematian dan kegagalan panen budidaya udang intensif. Salah satu upaya untuk mengendalikan prevalensi penyakit ini adalah dengan mengoptimalkan pemanfaatan senyawa metabolit sekunder dari daun mangrove. Penelitian ini bertujuan untuk mengetahui kandungan fitokimia dan aktivitas antibakteri ekstrak daun Avicennia marina dan Avicennia alba terhadap bakteri Vibrio sp. dari tambak udang intensif di sekitar Selat Madura. Ekstraksi daun mangrove dilakukan menggunakan metode maserasi. Aktivitas antibakteri diuji dengan metode difusi cakram pada konsentrasi 10.000 ppm, 40.000 ppm, dan 80.000 ppm. Penelitian ini dirancang dengan model rancangan acak lengkap (RAL) faktorial. Hasil penelitian menunjukkan bahwa kedua ekstrak mangrove mengandung senyawa saponin, tanin, dan triterpenoid. Zona hambat bakteri pada ekstrak A.marina berkisar 1,43 ± 0,72-4,31 ± 1,80 mm, sedangkan zona hambat pada A. alba berkisar 1,43 ± 0,72-4,71 ± 0,01 mm. Zona hambat yang terbentuk juga menunjukkan bahwa aktivitas antibakteri kedua ekstrak daun mangrove pada konsentrasi 10.000 ppm dan 40.000 ppm tergolong lemah, sedangkan 80.000 ppm tergolong sedang. Pemberian konsentrasi ekstrak yang berbeda berpengaruh nyata terhadap zona hambat bakteri yang dihasilkan, namun jenis ekstrak secara signifikan tidak terdapat interaksi nyata terhadap konsentrasi ekstrak mangrove yang digunakan. Secara keseluruhan hasil penelitian menunjukkan bahwa ekstrak daun A. marina dan A. alba dari Selat Madura berpotensi sebagai agen antibakteri alami terhadap bakteri Vibrio sp. pada budidaya udang intensif.Vibrio sp. bacteria are a group of pathogenic bacteria that can cause vibriosis, leading to high mortality rates and crop failure in intensive shrimp farming. One of the efforts to control the prevalence of this disease is by optimizing the use of secondary metabolites from mangrove leaves. This study aimed to determine the phytochemical content and antibacterial activity of Avicennia marina and Avicennia alba leaves extracts against Vibrio sp. bacteria from intensive shrimp ponds around the Madura Strait. Mangrove leaves extraction was carried out using the maceration method. Antibacterial activity was tested using the disc diffusion method at concentrations of 10,000 ppm, 40,000 ppm, and 80,000 ppm. The study was designed using a factorial completely randomized design (CRD). The results showed that both mangrove extracts contained saponins, tannins, and triterpenoids. The inhibition zone of bacteria in A. marina extract ranged from 1.43 ± 0.72 to 4.31 ± 1.80 mm, while in A. alba extract, it ranged from 1.43 ± 0.72 to 4.71 ± 0.01 mm. The inhibition zones also indicated that the antibacterial activity of both mangrove leaves extracts at concentrations of 10,000 ppm and 40,000 ppm was categorized as weak, while at 80,000 ppm it was categorized as moderate. Different extract concentrations had a significant effect on the bacterial inhibition zones produced; however, the type of extract showed no significant interaction with the concentrations of mangrove extract used. Overall, the results indicate that A. marina and A. alba leaves extracts from the Madura Strait have potential as natural antibacterial agents against Vibrio sp. in intensive shrimp farming.
Potensi Antibakteri Ekstrak Buah Mangrove Sonneratia Caseolaris Terhadap Bakteri Escherichia coli dari Perairan Tolbuk- Bangkalan Prasetyo, Adi; Ning Asih, Eka Nurrahema; Afnani, Fawait; Dewi, Kartika
Journal of Marine Research Vol 15, No 2 (2026): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v15i2.52733

Abstract

Escherichia coli merupakan bakteri patogen yang dapat menyebabkan gangguan kesehatan manusia seperti meningitis, infeksi saluran kemih, dan diare, serta menjadi penyebab utama kegagalan ekspor produk perikanan. Penelitian ini bertujuan untuk menganalisis kandungan senyawa fitokimia ekstrak buah mangrove Sonneratia caseolaris dan pontensi ekstrak ini sebagai anti bakteri E. coli dengan konsentrasi berbeda. Metode penelitian  ini  meliputi ekstraksi, perhitungan rendemen, uji fitokimia, serta uji zona hambat bakteri menggunaka overlay. Sampel diperoleh dari perairan Tolbuk-Bangkalan, dan diekstraksi menggunakan metode maserasi. Hasil penelitian menunjukkan rendemen ekstrak diperoleh sebesar 15,65%. Ekstrak buah S.caseolaris mengandung senyawa fitokimia berupa flavonoid, saponin, tanin, dan triterpenoid. Konsentrasi 100.000 ppm selama 24 jam merupakan konsentrasi dan waktu terbaik dalam menghasilkan zona hambat E.coli sebesar 6,81±1,82 mm dan masuk kategori sedang (p<0,05). Hasil penelitian ini diharapkan menjadi informasi awal tentang optimalisasi ekstrak buah S.caseolaris sebagai kandidat pangan sehat dan farmakologi untuk menghambat aktivitas E.coli. Escherichia coli is a pathogenic bacterium that causes human health issues such as meningitis, urinary tract infections, and diarrhea, and serves as a primary cause of fishery product export rejections. This study aimed to analyze the phytochemical content of Sonneratia caseolaris mangrove fruit extract and evaluate its potential as an antibacterial agent against E. coli at varying concentrations. Research methods included extraction, yield calculation, phytochemical screening, and bacterial inhibition zone testing using the overlay method. Samples were collected from Tolbuk waters in Bangkalan and extracted via maceration. Results showed an extract yield of 15.65%. The S.caseolaris fruit extract contained phytochemical compounds including flavonoids, saponins, tannins, and triterpenoids. A concentration of 100,000 ppm for 24 hours proved optimal, producing an E. coli inhibition zone of 6.81 ± 1.82 mm (classified as moderate; p < 0.05). These findings provide preliminary information on optimizing S. caseolaris fruit extract as a healthy food and pharmacology candidate to inhibit E. coli activity.