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Kualitas Pewarnaan Sediaan Apusan Darah Metode Giemsa Menggunakan Alternatif Pewarna Buah Naga Pengencer Air Mineral Monika Yati; Ahmad Muhlisin; Akhmad Muntaha; Erfan Roebiakto
Jurnal Karya Generasi Sehat Vol. 1 No. 1 (2023): Edisi Desember Tahun 2023
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v1i1.66

Abstract

Pewarna Giemsa pengencer buffer fosfat pH 6,8 adalah pewarnaan sediaan apusan darah dengan harga yang relatif mahal sehingga digunakan alternatif pewarna buah naga dan pengencer air mineral karena lebih aman dan ekonomis. Tujuan penelitian ini untuk mengetahui kualitas pewarnaan sediaan apusan darah menggunakan alternatif pewarna buah naga pengencer air mineral. Jenis penelitian adalah observasional laboratorium. Hasil pewarnaan Giemsa pengencer buffer fosfat (A), pewarna buah naga pengencer buffer fosfat (B), pewarna buah naga pengencer air mineral bermerek C, D, E dan F mempunyai kualitas pewarnaan yang baik berturut-turut sebesar 100%, 100%, 100%, 93%, 100%, 97%. Berdasarkan uji Mann-Whitney tidak ada perbedaan kualitas pewarnaan A dengan B sig p = 1,000 dan tidak ada perbedaan kualitas pewarnaan A dengan C, D, E, F sig p = 1,000 menggunakan uji Kruskal-Wallis. Disimpulkan bahwa pewarna buah naga pengencer air mineral bermerek dapat digunakan sebagai alternatif pewarna Giemsa dan pengencer buffer fosfat pH 6,8.
Perbedaan Hasil Tes Darah Rutin Pasien Suspek Covid-19 Terkonfirmasi Positif Dan Negatif Di RSUD Sultan Suriansyah Banjarmasin Yayuk Kustiningsih; Ida Fitriyani; Erfan Roebiakto; Anny Thuraidah; Nurlailah
Jurnal Praba : Jurnal Rumpun Kesehatan Umum Vol. 3 No. 3 (2025): September : Jurnal Praba : Jurnal Rumpun Kesehatan Umum
Publisher : STIKES Columbia Asia Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62027/praba.v3i3.576

Abstract

The purpose of this study was to determine the differences in routine blood in COVID-19 suspected patients who were confirmed positive and negative who were treated at the Emergency Room of Sultan Suriansyah Hospital Banjarmasin in the period January-June 2022. This study used naso-oropharyngeal swab samples for RT-PCR examination of COVID-19 and hematology analyzer for routine blood tests. This type of research is an analytic survey research with a comparative design. The population of this study were suspected patients admitted to the emergency room with a total sample size of 196 patients who performed RT-PCR tests and performed routine blood tests at the beginning of the treatment period using purposive sampling method. Routine blood results in positive patients mostly obtained leukocytes at normal limits 64.1%, neutrophilia 59.0%, lymphopenia 64.1% increased NLR 66.7% and platelets at normal limits 56.4%. While in negative patients, leukocytes were obtained at normal limits of 46.6%, neutrophilia 61.0%, lymphopenia 61.0% increased NLR 73.7% and platelets at normal limits 64.4.4%. Based on the results of the unpaired T-test statistical test, it is known that there are significant differences between positive and negative patients, namely in the parameters of leukocytes sig. 0.028, neutrophils sig. 0.043 and platelets sig. 0,005. From the results of the unpaired t-test statistical test on routine blood between positive and negative COVID-19 patients, it was found that there were significant differences in the parameters of leukocytes, neutrophils and platelets, while those that were not different were the parameters of lymphocytes and NLR.