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All Journal Proceeding International Conference Of Innovation Science, Technology, Education, Children And Health Proceeding International Conference Of Innovation Science, Technology, Education, Children And Health Prosiding Seminar Nasional Ilmu Kesehatan dan Keperawatan
Henry Kurnia Setiawan
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Computer Science & IT Education Engineering Nursing Public Health Social Sciences Other

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Validasi Metode Identifikasi Fenilbutazon dan Deksametason dalam Jamu Pegal Linu secara Kromatografi Lapis Tipis-Densitometri Imelda Rindiantyas; Diana Diana; Henry Kurnia Setiawan
Prosiding Seminar Nasional Ilmu Kesehatan dan Keperawatan Vol. 1 No. 1 (2024): Juni : Prosiding Seminar Nasional Ilmu Kesehatan dan Keperawatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/prosemnasikk.v1i1.4

Abstract

Traditional herbal medicine, known as 'Jamu,' is prohibited from containing pharmaceutical chemical substances (BKO) by the Indonesian Food and Drug Monitoring Agency (BPOM). In October 2022, BPOM released a public warning after discovering the presence of phenylbutazone and dexamethasone in a muscle pain relief jamu product. The purpose of this research is to establish a valid method for identifying phenylbutazone and dexamethasone in jamu pegal linu. In this study, the method was applied to 10 brands of muscle pain relief jamu samples. The samples were extracted with methanol and then spotted in a quantity of 5 μl on a silica gel 60 F254 plate, eluted with a selected mobile phase, namely ethyl acetate: methanol: ammonia (8.5:1:0.5, v/v/v). Spots were observed with a densitometry at λ240 nm. Phenylbutazone appeared at Rf 0.33, and dexamethasone at Rf 0.77, with a resolution value (Rs) of 5,8. The detection limits for phenylbutazone were 8,5307 μg/ml (0,1706 mg/600 mg capsule) and for dexamethasone, 8,7611 μg/ml (0,1752/600 mg capsule). The results revealed that two samples tested positive for both phenylbutazone and dexamethasone, while six samples tested positive for dexamethasone.
Analytical Method Validation of Sildenafil Citrate and Caffeine in Herbal Medicine for Increasing Stamina using Thin Layer Chromatography - Densitometry Diana, Diana; Rena Octaria; Henry Kurnia Setiawan
Proceeding International Conference Of Innovation Science, Technology, Education, Children And Health Vol. 5 No. 1 (2025): Proceeding of The International Conference of Inovation, Science, Technology, E
Publisher : Program Studi DIII Rekam Medis dan Informasi Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62951/icistech.v5i1.277

Abstract

Traditional medicine has been believed for generations to be effective in treating various diseases and is recognized for its safety. One reason people opt for herbal medicine is its affordability compared to chemical drugs. Additionally, herbal medicine is required to be free of medicinal chemicals (BKO). However, a public warning issued between 2020 and 2025 indicated that some herbal preparations still contained substances like sildenafil citrate and caffeine. The aim of this research is to establish a validated analytical method to detect the presence of sildenafil citrate and caffeine in stamina-enhancing herbal medicines using Thin Layer Chromatography (TLC) with densitometry. This method was selected due to its cost-effectiveness and its ability to separate two compounds. In this study, category II validation was conducted to identify impurities in the preparations, focusing on test parameters such as selectivity and Limit of Detection (LOD). The selected mobile phase for this study was a mixture of chloroform and ethanol (9.5:0.5 v/v). The TLC plate was analyzed with a densitometer at a wavelength of 254 nm. The Rf value for sildenafil citrate was found to be 0.32, while the Rf value for caffeine was 0.52, resulting in a separation (Rs) value of 2. The LOD for sildenafil citrate was determined to be 8.5418 μl/ml (equivalent to 0.0854 mg in a 600 mg sample), whereas the LOD for caffeine was 4.7987 μl/ml (or 0.0959 mg in a 600 mg sample).
Analytical Method Validation of Sildenafil Citrate and Caffeine in Herbal Medicine for Increasing Stamina using Thin Layer Chromatography - Densitometry Diana, Diana; Rena Octaria; Henry Kurnia Setiawan
Proceeding International Conference Of Innovation Science, Technology, Education, Children And Health Vol. 5 No. 1 (2025): Proceeding of The International Conference of Inovation, Science, Technology, E
Publisher : Program Studi DIII Rekam Medis dan Informasi Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62951/icistech.v5i1.277

Abstract

Traditional medicine has been believed for generations to be effective in treating various diseases and is recognized for its safety. One reason people opt for herbal medicine is its affordability compared to chemical drugs. Additionally, herbal medicine is required to be free of medicinal chemicals (BKO). However, a public warning issued between 2020 and 2025 indicated that some herbal preparations still contained substances like sildenafil citrate and caffeine. The aim of this research is to establish a validated analytical method to detect the presence of sildenafil citrate and caffeine in stamina-enhancing herbal medicines using Thin Layer Chromatography (TLC) with densitometry. This method was selected due to its cost-effectiveness and its ability to separate two compounds. In this study, category II validation was conducted to identify impurities in the preparations, focusing on test parameters such as selectivity and Limit of Detection (LOD). The selected mobile phase for this study was a mixture of chloroform and ethanol (9.5:0.5 v/v). The TLC plate was analyzed with a densitometer at a wavelength of 254 nm. The Rf value for sildenafil citrate was found to be 0.32, while the Rf value for caffeine was 0.52, resulting in a separation (Rs) value of 2. The LOD for sildenafil citrate was determined to be 8.5418 μl/ml (equivalent to 0.0854 mg in a 600 mg sample), whereas the LOD for caffeine was 4.7987 μl/ml (or 0.0959 mg in a 600 mg sample).
Analytical Method Validation of Sildenafil Citrate and Caffeine in Herbal Medicine for Increasing Stamina using Thin Layer Chromatography – Densitometry Rena Octaria; Diana Diana; Henry Kurnia Setiawan
Proceeding International Conference Of Innovation Science, Technology, Education, Children And Health Vol. 5 No. 1 (2025): Proceeding of The International Conference of Inovation, Science, Technology, E
Publisher : Program Studi DIII Rekam Medis dan Informasi Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62951/icistech.v5i1.279

Abstract

Traditional medicine has been trusted for generations as an effective remedy for various health conditions and is widely recognized for its safety when compared to synthetic pharmaceuticals. One of the main reasons individuals choose herbal medicines is their relative affordability and the perception that they are free from harmful chemical additives. However, recent public warnings issued between 2020 and 2025 have revealed the presence of hazardous medicinal chemicals (Bahan Kimia Obat/BKO) such as sildenafil citrate and caffeine in some stamina-enhancing herbal products. This study aims to develop and validate an analytical method using Thin Layer Chromatography (TLC) coupled with densitometry for the detection of sildenafil citrate and caffeine in herbal medicines. The method was chosen due to its simplicity, low operational cost, and ability to effectively separate these two compounds. A category II validation was performed to determine the presence of impurities, with a focus on parameters including selectivity and the Limit of Detection (LOD). The mobile phase employed was a combination of chloroform and ethanol (9.5:0.5 v/v), and detection was carried out at 254 nm using a densitometer. The TLC results showed that sildenafil citrate and caffeine had Rf values of 0.32 and 0.52, respectively, with a resolution (Rs) of 2, indicating a good separation. The LOD for sildenafil citrate was 8.5418 μl/ml (0.0854 mg per 600 mg sample), while the LOD for caffeine was 4.7987 μl/ml (0.0959 mg per 600 mg sample). These findings highlight the importance of routine quality control testing to ensure the safety and authenticity of herbal medicines in the market.
Analytical Method Validation of Sildenafil Citrate and Caffeine in Herbal Medicine for Increasing Stamina using Thin Layer Chromatography – Densitometry Rena Octaria; Diana Diana; Henry Kurnia Setiawan
Proceeding International Conference Of Innovation Science, Technology, Education, Children And Health Vol. 5 No. 1 (2025): Proceeding of The International Conference of Inovation, Science, Technology, E
Publisher : Program Studi DIII Rekam Medis dan Informasi Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62951/icistech.v5i1.279

Abstract

Traditional medicine has been trusted for generations as an effective remedy for various health conditions and is widely recognized for its safety when compared to synthetic pharmaceuticals. One of the main reasons individuals choose herbal medicines is their relative affordability and the perception that they are free from harmful chemical additives. However, recent public warnings issued between 2020 and 2025 have revealed the presence of hazardous medicinal chemicals (Bahan Kimia Obat/BKO) such as sildenafil citrate and caffeine in some stamina-enhancing herbal products. This study aims to develop and validate an analytical method using Thin Layer Chromatography (TLC) coupled with densitometry for the detection of sildenafil citrate and caffeine in herbal medicines. The method was chosen due to its simplicity, low operational cost, and ability to effectively separate these two compounds. A category II validation was performed to determine the presence of impurities, with a focus on parameters including selectivity and the Limit of Detection (LOD). The mobile phase employed was a combination of chloroform and ethanol (9.5:0.5 v/v), and detection was carried out at 254 nm using a densitometer. The TLC results showed that sildenafil citrate and caffeine had Rf values of 0.32 and 0.52, respectively, with a resolution (Rs) of 2, indicating a good separation. The LOD for sildenafil citrate was 8.5418 μl/ml (0.0854 mg per 600 mg sample), while the LOD for caffeine was 4.7987 μl/ml (0.0959 mg per 600 mg sample). These findings highlight the importance of routine quality control testing to ensure the safety and authenticity of herbal medicines in the market.
Co-Authors Diana Diana Imelda Rindiantyas Rena Octaria