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All Journal Jurnal Sains Teh dan Kina
Fani Fauziah
Pusat Penelitian Teh dan Kina
Agriculture, Biological Sciences & Forestry Computer Science & IT Environmental Science Industrial & Manufacturing Engineering Mechanical Engineering

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Analysis of Tea Rhizosphere Bacterial Community at the Seedling Stage Using Terminal Restriction Fragment Length Polymorphism (TRFLP) Techniques Dwi Ningsih Susilowati; Fani Fauziah; Eko Pranoto; Ernin Hidayat; Mamik Setyowati; Yati Rachmiati
Jurnal Penelitian Teh dan Kina Vol 19 No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v19i2.114

Abstract

Bio-imunizer contains an active compound of  Chryseobacterium sp. and Bacillus sp. has been developed by PPTK Gambung. This formula has positive effect on the growth of tea plants also potentially increasing resistance of the plant. The purpose of this study was to determine the effects of bacteria in Bio-imunizer to the rhizosphere bacterial communities as well as the consistency of its existence after application on tea plants at the nursery stage. The technique used in this research is Terminal Restriction Fragment Length Polymorphism based on metagenomic and culture dependent approaches. The value of relative abundance, Shannon diversity index, Pielou's evenness index, and Simpson dominance index were calculated. Based on the T-RF profiles of rhizosphere bacterial communities show that Chryseobacterium sp. and Bacillus sp. which is the active compound of  Bio-imunizer consistently found in the tea plant rhizosphere. Application of Bio-imunizer can increase the diversity of rhizosphere bacterial community without affecting the communities that already exist.
Uji Beberapa Senyawa Volatil Sebagai Atraktan Empoasca flavescens di Perkebunan Teh Fathiannisa; Fani Fauziah
Jurnal Penelitian Teh dan Kina Vol 2 No 2 (2023): Jurnal Sains Teh dan Kina
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Wereng pucuk teh, Empoasca flavescens, merupakan hama utama yang umum di perkebunan teh. Analisis senyawa volatil yang dihasilkan oleh tanaman teh telah dilakukan untuk mengidentifikasi senyawa volatil yang berpotensi sebagai atraktan E. flavescens. E. flavescens diketahui tertarik pada 3-hexen-1-ol, β-ocimene, dan metil salisilat. Penelitian ini merupakan tahap awal pengembangan formulasi atraktan, yaitu pengujian respons perilaku E. flavescens terhadap senyawa-senyawa volatil di laboratorium dan pengujian di lapangan. Hasil percobaan di laboratorium menunjukkan bahwa senyawa 3-hexen-1-ol memiliki merupakan senyawa yang paling disukai oleh E. flavescens. Sementara itu, uji lapangan menunjukkan bahwa modifikasi sticky trap berwarna merah dan senyawa volatil lebih menarik bagi E. flavescens dibandingkan kontrol. Perlakuan 3-hexen-1-ol memiliki persentase E. flavescens yang terperangkap paling tinggi dengan rata-rata Empoasca yang terperangkap sebanyak 0,3 - 5 ekor/perangkap.
Co-Authors Dwi Ningsih Susilowati Eko Pranoto Ernin Hidayat Fathiannisa Mamik Setyowati Yati Rachmiati