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All Journal Biospecies Jurnal Sains Teh dan Kina PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia)
Eko Pranoto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering

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Isolasi dan Karakterisasi Bakteri Endofit Pada Tanaman Teh (Camellia Sinensis (L.) O. Kuntze) Produktif dan Belum Menghasilkan Klon GMB 7 Dataran Tinggi Eko Pranoto; Gilang Fauzi; Hingdri Hingdri
Biospecies Vol. 7 No. 1 (2014): Januari 2014
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v7i1.1489

Abstract

Tujuan penelitian ini adalah untuk mengeksplorasi bakteri endofit pada tanaman tehbelum menghasilkan klon GMB 7 pada dataran tinggi di Pusat Penelitian Teh dan Kina, Gambung.Penelitian dilaksanakan di Laboratorium Mikrobiologi PPTK Gambung. Isolasi dilakukan dengancara metode sebar dan metode tuang. Sumber isolat diambil dari bagian daun, batang, dan akartanaman. Media yang digunakan adalah media TSA (Trypic Soy Agar). Pengamatan morfologimakroskopik dilakukan untuk mengamati bentuk koloni bakteri endofitik tersebut. Dari hasilpenelitian diperoleh jumlah isolat bakteri endofit pada TBM sebanyak 13 isolat, yaitu isolat padabagian daun terdapat lima isolat, empat isolat pada bagian batang dan bagian akar. Dari hasilpengamatan terdapat satu isolat dengan karakteristik yang hampir sama pada setiap jaringan(daun, batang, dan akar) tanaman. Selain itu juga terdapat isolat bakteri yang memiliki filamenfilamendi sekitar koloni yaitu isolat yang berasal dari bagian akar tanaman teh yang belummenghasilkan. Sedangkan bakteri endofit pada TM sebanyak 11 isolat, yaitu pada bagian daunterdapat tiga isolat, pada bagian batang tiga isolat dan pada bagian akar lima isolat. Dari hasilpengamatan terdapat satu isolat dengan karakteristik yang hampir sama pada setiap jaringan(daun, batang, dan akar) tanaman. Dan juga terdapat isolat bakteri yang memiliki filamen-filamendi sekitar koloni yaitu isolat yang berasal dari bagian akar tanaman teh menghasilkan, sama halnyadengan tanaman teh belum menghasilkan.
Pola Sebaran Populasi Azotobacter sp dan Bahan Organik pada Berbagai Kelas Kemiringan Lereng Perkebunan Teh Dataran Tinggi PPTK Gambung (Population Distribution Pattern of Azotobacter sp and Organic Material on Variety Slope Classification of Tea Highland Eko PRANOTO; Sulistiawati PRATIWI; Hani WACHYUNI; Sastrika ANINDITA
Biospecies Vol. 8 No. 1 (2015): Januari 2015
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v8i1.2201

Abstract

The purpose of this experiment was to identified the population distribution of Azotobacter sp and organic matter on variety slope classification at experimental field, PPTK Gambung, West Java. The methode that used was exploration method. PPTK Gambung’s tea plantation has three class of slope, they are slope of <8% (flat-rather slope slighty), 8-15% (slope slighty), and 16-25% (rather steep). Total 14 composite soil sample taken from each of the class to analyze total population of Azotobacter sp, average of organic matter soil content, and water content average. Sample was taken based on sample point in Land Unit Map (SPL). Slopes <8% (flat-rather slope slighty) have total population of Azotobacter sp around 7,14x107 cfu/gram soil, water content 5,97%, and organic matter 10,68%. Slopes 8-15% (slope slighty) have total population of Azotobacter sp around 10,38x107 cfu/gram soil, water content 6,47%, and organic matter 10,91%. And slopes 16-25% (rather steep) have total population of Azotobacter sp around 11,75x107 cfu/gram soil, water content 6,34%, and organic matter 10,65%.
INFEKSI SALURAN KEMIH DI INSTALASI RAWAT INAP RSUD BANYUMAS PERIODE AGUSTUS 2009 – JULI 2010 Eko Pranoto; Anis Kusumawati; Indri Hapsari
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 09 No. 02 Agustus 2012
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pji.v9i2.694

Abstract

ABSTRAK Infeksi Saluran kemih ( ISK ) adalah keadaan dimana saluran perkemihan terinfeksi oleh patogen yang menyebabkan inflamasi. ISK biasa ditemukan pada masa anak anak hingga manula. Pasien ISK bergejala menerima terapi antimikroba. Ada banyak jenis antibiotika dari bermacam macam golongan dalam pengobatan ISK. Penelitian ini dianalisis menggunakan metode retrospektif pada 115 pasien rawat inap RSUD Banyumas Periode Agustus 2009 – Juli 2010 dan dibandingkan dengan Pedoman Diagnosa dan Terapi RSUD Banyumas. Hasil menunjukan, penderita ISK adalah 12,17% pediatrik, 71,30% dewasa dan 16,52% Lansia. Sebanyak 74,78% pasien menunjukan positif bakteriuria dan 25,12% negatif. Terdapat 12 jenis antibiotika yang digunakan dalam 115 kasus. Antibiotika Ceftriakson sebesar 59,38% merupakan antibiotika yang banyak digunakan. Kata kunci: Antibiotika, Pasien, ISK, RSUD Banyumas ABSTRACT Urinary Tract Infection ( UTI ) is a condition in which the urinary tract is infected with a pathogen causing inflammation. UTI is commonly found on children to geriatric periods. Symptomatic UTI patient should receive an antimicrobial therapy. There is a lot of agents from many groups of antibiotic commonly used which are treat UTI. This research analized with retrospectif methods to 115 hospitalized patient at RSUD Banyumas in August 2009 – July 2010 and compared to Pedoman Diagnosa dan Terapi RSUD Banyumas. The result shows, UTI patient are 12,17% pediatric, 71,30% adult and 16,52% geriatric. In amount of 74,78% patient show positif bacteriuria and 25,12% negatif. There is twelve antibiotic agents are used by 115 cases. Ceftriaxone 59,38%, is poppular antibiotic. Keywords: Antibiotic, patient, UTI, RSUD Banyumas.
The comparison of effectivity from consortium bio fertilizer and endophytic bio fertilizer on productivity and health of clone mature GMB 7 tea crop Mieke Rochimi Setiawati; Restu Wulansari; Eko Pranoto
Jurnal Penelitian Teh dan Kina Vol 17 No 2 (2014)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v17i2.48

Abstract

This study aimed at comparing the effec­tiveness of consortium bio-fertilizer with endo­phytic bio fertilizer to increasing produc­tivity and health of mature clone GMB 7 tea crop. This research was carried out at the Gambung Experimental Station Block A7 Research Insti­tute for Tea and Cinchona, from July 2013 to September 2013. Randomized block design was used in this study with ten treatments and three replications i.e.: A1H0 = anorganic fertilizer (75%), A2H0 = anorganic fertilizer (50%), A1H1 = anorganic fertilizer (75%) + 2 liter consortium bio fertilizer, A1H2 = anorganic fertilizer (75 %) + 4 liter consor­tium bio-ferti­lizer, A2H1 = anorganic fertilizer (50 %) + 2 liter consortium bio fertilizer, A2H2 = anorga­nic fertilizer (50%) + 4 liter consor­tium bio-fertilizer, A1H3 = anorganic fertilizer (75 %) + 2 liter endophytic bio fertilizer, A1H4 = anor­ganic fertilizer (75%) + 4 liter endo­phytic bio fertilizer, A2H3 = anorganic fertili­zer (50%) + 2 liter endophytic bio fertilizer and A2H4 = anorganic fertilizers (50%) +4 liter endophytic bio fertilizer. The results showed there were a significantly difference on amount of the pekoe shoot among treatments, although the others variable was not significantly diffe­rent. However, the overall consortium bio-fertilizer resulted in the production of shoots 15,36% and endophytic biofertilizer application resulted in the production of shoots 21,93% higher than the control (without bio fertilizer). When compared to the first plucking, the num­ber of shoot from July to September showed increasing trend, so did the number of banji shoots.
The application of technology portable fertigation system in the dry month in tea plantation Pudjo Rahardjo; Restu Wulansari; Eko Pranoto
Jurnal Penelitian Teh dan Kina Vol 18 No 1 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v18i1.56

Abstract

The research aims at providing multi­function both irrigation and fertilizer appli­cations (fertigation) and calculates the produc­tion of shoots and evaluated plant health during dry season. This research was carried out on Gambung Research Station using clone GMB7. Randomized block design was used in this study with six treatments and four replications. The treatments i.e. a. control (without irrigation, without fertilization); b. without irrigation, fer­ti­lization through the soil; c. irrigation 7 mm/day every 3 days (without fertilization); d. irrigation 7 mm/day every 6 days (without fertilization); e. fertigation 7 mm/day every 3 days; and f. fertigation 7 mm/day every 6 days. Observations were made from September-October 2014 with 4 times of plucking. All treatments were significantly different on the yield, number of both pecco and banji. The fertigation treatment using 21 mm of water (7 mm/day every 3 days) showed the highest shoot production of 15,56%. The highest number of pecco shoots and ratio of the number pecco/number of banji in treatment using 21 mm of water (7 mm/day every 3 days) of 32,25%. The highest number of banji found in the control treatment (without irrigation without fertilization) was 74,94%. From this research it can be concluded that the fertigation treatment can give good impact to production maintain and plant health in the dry season.
Potency of tea plant indigenous microbe on plant growth and to against blister blight disease (Exobasidium vexans Massee) Fani Fauziah; Mieke Rochimi Setiawati; Dwi Ningsih Susilowati; Eko Pranoto; Yati Rachmiati
Jurnal Penelitian Teh dan Kina Vol 19 No 1 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v19i1.77

Abstract

The chemical control method of blister blight (Exobasidium vexans Massee) on tea could inflict various negative impacts. In order to obtain an environmentally sound control method of blister blight disease, a nursery trial has been conducted to know the effectiveness of bacterial combinations. The trial was carried out at Gambung experimental garden, with seven treatments and four replications. The indigenous microbial codes are Azoto II-1, Endo-5 and Endo-76. The treatments tested comprised: control (without bacteria),  Azoto II-1 25% + Endo-5 75%; Azoto II-1 50% + Endo-5 50%; Azoto II-1 75% + Endo-5 25%;  Azoto II-1 25% + Endo-76 75%; Azoto II-1 50% + Endo-76 50%; and Azoto II-1 75% + Endo-76 25%. All of the treatments was applied as a soil drench, 50 ml/plant with bacterial concentration at 0,5%. The parameter observed was blister blight disease intensity, plant heights, stem diameter, leaves number, root length, and root volume. The results showed that the combination of Azoto II-1 75% + Endo-5 25% could suppress the intensity of blister blight disease with disease intensity 1.27%. The treatments also affected plant heights, stem diameter, leaves number, root length, and root volume, 15.32 cm; 3.38 cm; 8.05 cm; 18.25 cm and 2.37 cm, respectively.
Comparison of some exogeneous solubillizing phosphate bacteria on Andisol as Indonesia dominant native tea soil Mieke Rochimi Setiawati; Eko Pranoto
Jurnal Penelitian Teh dan Kina Vol 18 No 2 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v18i2.78

Abstract

Tea was planting an upland area with Andisol as the dominant soil ordo. Andisol has a special characteristic i.e. low soil pH and it has more mineral that causing low of soil phos-phate soluble, especially for tea plants, there-fore the rule of solubilizing phosphate bacteria will be needed. Laboratory scale experiments were conducted to study the capability activities of some solubilizing phosphate bacterias name-ly Pseudomonas cepaceae, P. malei, Bacillus mycoides, and B. subtilis. The experiment was arranged in Completely Randomized Design with six treatments and four replications. The result on statistical analysis shown that there were no significant differences in increasing of the soil phosphate soluble. Nevertheless, the treatment D (Bacillus mycoides) produced the highest soil phosphate soluble. The capability of bacteria to increase soil phosphate soluble was depended on its habitat, especially soil pH. Even all of the isolates were the superior solubilizing phosphate bacterias in agricultural food plants with neutral soil pH, but they have not significantly different on Andisol. There-fore, the using of indigenous isolates on tea plant areas was suggested.
Exploration and Effectiveness Test from Some Tea Indigenous Potassium Soluble Bacteria Eko Pranoto; Restu Wulansari; Faris Nur Fauzi Athallah; Fitrianti Widya Lestari
Jurnal Penelitian Teh dan Kina Vol 19 No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v19i2.110

Abstract

Tea is a plant that benefits for health. The growth of tea plant was influenced by several factors, including superior clones and nutrient availability in soil. One of the most important macro nutrients is potassium. However, the availability of potassium is low because of retention on the soil. One of the methode for absorbed by plant was used Potassium Solubilizing Bacteria (PSB) with its organic acid function. The aim of the study was to explore and determine the density of colonies (DC) and the potential of Tea Indigenous PSB. Inoculum source from tea rhizosphere and rhizoplan of GMB 7, GMB 9 and TRI 2024 clones. The experimental design used was completely randomized design with six treatments and four replications, i.e. : GMB 7 Rhizosphere; GMB 7 Rhizoplan; GMB 9 Rhizosphere; GMB 9 Rhizoplan; TRI 2024 Rhizosphere; and TRI 2024 Rhizoplan. Ten isolates with the highest halozone was tested by the Dissolution Index (DI) of the PSB. The result showed that the highest DC was GMB 9 rhizoplan by 10.9 x 105 CFU/g and the lowest was TRI 2024 rhizoplant by 3.1 x 105 CFU/g. The DC on GMB clones series showed that rhizoplan was 14,29% higher than rhizosphere. There is no significant difference of DI for PSB colonies from all clones. From that clones, we were obtained 10 isolat that have the highest halozone, that were 2 isolates from rhizosphere and 8 isolates from rhizoplan. The average of DI rhizosphere isolates was 119,9% higher than rhizoplan.
Analysis of Tea Rhizosphere Bacterial Community at the Seedling Stage Using Terminal Restriction Fragment Length Polymorphism (TRFLP) Techniques Dwi Ningsih Susilowati; Fani Fauziah; Eko Pranoto; Ernin Hidayat; Mamik Setyowati; Yati Rachmiati
Jurnal Penelitian Teh dan Kina Vol 19 No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v19i2.114

Abstract

Bio-imunizer contains an active compound of  Chryseobacterium sp. and Bacillus sp. has been developed by PPTK Gambung. This formula has positive effect on the growth of tea plants also potentially increasing resistance of the plant. The purpose of this study was to determine the effects of bacteria in Bio-imunizer to the rhizosphere bacterial communities as well as the consistency of its existence after application on tea plants at the nursery stage. The technique used in this research is Terminal Restriction Fragment Length Polymorphism based on metagenomic and culture dependent approaches. The value of relative abundance, Shannon diversity index, Pielou's evenness index, and Simpson dominance index were calculated. Based on the T-RF profiles of rhizosphere bacterial communities show that Chryseobacterium sp. and Bacillus sp. which is the active compound of  Bio-imunizer consistently found in the tea plant rhizosphere. Application of Bio-imunizer can increase the diversity of rhizosphere bacterial community without affecting the communities that already exist.
Degradasi bahan organik di beberapa perkebunan teh di Jawa Barat Restu Wulansari; Eko Pranoto
Jurnal Penelitian Teh dan Kina Vol 21 No 2 (2018): Jurnal Penelitian Teh dan Kina
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v21i2.144

Abstract

The tea production and the quality were over the years, especially in West Java, Indonesia. This had been affected by several factors including the aged of tea plantations, declining soil fertility, and soil degradation among other factors. Andisol is the most suitable soil and dominant in Indonesia for tea plantation. The objectives of this study were to evaluate the impact of long-term tea cultivation on soil organic matter degradation in 6 years (2011 and 2017) in tea plantations in Bandung, Bogor, Cianjur and Garut, West Java. Data analysis used the independent sample t-test with SPSS 16.0 at a significant level of 95%. This study used a quantitative descriptive method by comparing the levels of organic matter and macronutrients from 89 samples taken in 2011 and 2017. Soil samples were taken at a depth of 35-40 cm with a distance of 25cm of the tea plant. Conclusion of the study, that the levels of organic matter in the regions of Bandung, Cianjur, and Garut are not significantly different between 2011 and 2017; while in the Bogor area the level of organic matter in 2011 was 5.41% and decreased in 2017 was 4.40% (a decrease of 18.72%). However, based on C-organic data in 2011 and 2017 nutrient degradation has occurred in all locations. Decreasing organic matter can cause a low decrease in soil fertility and productivity of tea crops. Provision of organic matter and proper fertilization must be done to maintain soil fertility and productivity of tea plants.
Co-Authors Anis Kusumawati DWI NINGSIH SUSILOWATI Dwi Ningsih Susilowati Ernin Hidayat Fani Fauziah Fani Fauziah Faris Nur Fauzi Athallah Fitrianti Widya Lestari Gilang Fauzi Hani WACHYUNI Hingdri Hingdri Indri Hapsari Mamik Setyowati Mieke Rochimi Setiawati Pudjo Rahardjo Restu Wulansari Restu Wulansari Sastrika ANINDITA Sulistiawati PRATIWI Yati Rachmiati