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All Journal Berkala Ilmiah Biologi
Dhia Salsabila Hakim
Fakultas Biologi Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Identifikasi dan Seleksi Fungi Endofit Daun Sirih Hijau (Piper betle L.) Penghasil Enzim Selulase Dhia Salsabila Hakim; Rina Sri Kasiamdari
Berkala Ilmiah Biologi Vol 14 No 3 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i3.8714

Abstract

Abstract: Endophytic fungi were known in bioethanol industries due to their role in secreting secondary metabolites in the form of cellulolytic enzymes, which were known to be superior to bacteria. The secretion of metabolic enzymes depended on the host they invaded. Herbal plants were recognized as habitats for various endophytic fungi. Betel leaf (Piper betle L.) was one such herbal plant known for its high metabolite content. The aim of this study was to identify endophytic fungi living on betel leaf, conduct cellulolytic tests on the identified fungi, and determine which fungi exhibited the highest cellulolytic activity. The study yielded three species of endophytic fungi from betel leaf: Fusarium graminearum, Fusarium oxysporum, and Colletotrichum coccodes. All three samples showed positive results, producing cellulase enzymes characterized by the presence of a clear zone around the colonies. F. oxysporum was identified as the highest producer of cellulase enzymes, with a concentration of 0,033 U/ml.
Co-Authors Rina Sri Kasiamdari