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ANTIBIOTICS SENSITIVITY TEST DIFFUSION AND DILUTION METHODS Nadia Elzuhria A; Nur Silmi kaffah; Nafis Rizqi N; Ummi Hanidah; Aisyah Mallika S; Firda Ayu Nabilla; Fajwatunnisa Azkadhafina; Taufiq Ahmad Hidayat; Nadya Fierdini Putri; Putri Alifia Zahra; Tristan Setiawan; Fomaembarasi Buulolo
Journal of Research in Pharmacy and Pharmaceutical Sciences Vol 2 No 1 (2023): Vol.2 No.1 JUNE (2023)
Publisher : Pharmacy Program, Faculty of Medicine, UPN Veteran Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jrpps.v2i1.7027

Abstract

An antibiotic sensitivity test is a method to test the response of bacteria to antibiotics. This study aims to determine the effectiveness of an antibiotic against microbial activity. The sensitivity test was conducted with two methods, namely, the diffusion method and the dilution method. The diffusion method was performed using paper discs (Kirby-Bauer) against Escherichia coli and Shigella sonnei bacteria with amoxicillin, neomycin, and sulfonamide antibiotics. The data required is the diameter of the inhibition zone. Results showed that Escherichia coli was sensitive to amoxicillin but resistant to sulfonamide and neomycin. Meanwhile, Shigella sonnei was resistant to amoxicillin, neomycin, and sulfanilamide. Furthermore, the dilution method was performed to test the potency of amoxicillin against Escherichia coli bacteria using the liquid dilution method. The data required were test tubes with liquid media that showed no turbidity. The results showed that the minimal inhibitory concentration of amoxicillin against Escherichia coli was 0.25%. Based on the results of the antibiotic sensitivity test using the diffusion and the dilution methods, it can be concluded that amoxicillin has high effectiveness against Escherichia coli bacteria with a minimum inhibitory concentration of 0.25%, while Shigella sonnei is resistant to the antibiotics tested.
EFFECTIVENESS OF IRON SUPPLEMENTATION PROGRAMS IN PREVENTING ANEMIA AMONG PREGNANT WOMEN: SYSTEMATIC LITERATURE REVIEW Nasywa Dwi Ayu Suhendi; Ummi Hanidah; Nafis Ikhwana; Brooklyn Razan; Deskia Amanda
Journal of Research in Pharmacy and Pharmaceutical Sciences Vol 4 No 2 (2025): Vol.4 No.2 DECEMBER (2025)
Publisher : Pharmacy Program, Faculty of Medicine, UPN Veteran Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jrpps.v4i2.12418

Abstract

Anemia in pregnancy remains a major public health issue in Indonesia, contributing to maternal and fetal complications. This study aims to assess the effectiveness of iron supplementation in preventing anemia among pregnant women through a systematic literature review using PRISMA guidelines. A total of 12 eligible articles published from 2016 to 2025 were analyzed, focusing on the Indonesian population. The findings consistently demonstrate that iron and folic acid supplementation significantly increases hemoglobin levels and reduces anemia prevalence in pregnant women. However, adherence to supplementation is a persistent challenge. Only 53.4% of pregnant women consumed iron tablets for at least 90 days, with adherence influenced by maternal education, socioeconomic status, access to healthcare, and family support. While most studies confirmed the biological effectiveness of iron supplementation, limitations such as small sample sizes, limited geographic coverage, lack of control groups, and short-term observation periods were noted. To optimize outcomes, a multidimensional approach involving health education, improved healthcare access, and community support is essential. Strengthening adherence and monitoring mechanisms can enhance the long-term impact of iron supplementation in reducing maternal anemia in Indonesia.