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The Effect of Fermentation Time on The Quality of Mocaf (Modified Cassava Flour) with Raw Material Bokor Genotype Cassava Erfa Kurnia Prastiwi; Rois Fatoni; Ahmad Fathoni; R. Haryo Bimo Setiarto; Ema Damayanti
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 13, No 1 (2024): March 2024
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v13i1.12-26

Abstract

Cassava genotype Bokor has a fairly high content of beta carotene, which is beneficial for health and can also support the development of the functional food industry. MOCAF (Modified Cassava Flour) is a product made from cassava flour which is processed by modifying cassava cells through fermentation. This study aims to analyze the effect of long fermentation time on the characteristics of MOCAF from beta-carotene-rich cassava genotype Bokor. In this study, three variations of fermentation time were used, namely 15 hours, 24 hours and 40 hours. The research results showed that the time of fermentation affected the physicochemical properties of MOCAF. NIR analysis showed that the fermentation time affected the chemical composition of MOCAF. At 40 hours (last time of fermentation), there was a decrease in fat content with a value of 1.59% and an increase in water, protein and fibre content with a value of 13.71%, 3.94% and 2.28%, respectively. Proximate analysis showed that there was an effect of the length of fermentation time on the MOCAF content, namely at 40 hours, there was an increase in water content with a value of 10.10%, a decrease in dry matter, ash, protein, fat, fibre with a value of 89.90%, 0.60%, 0.78% respectively, 0.45%, and 1.29%. Scanning Electron Microscope (SEM) analysis shows the effect of fermentation time on the morphological properties of MOCAF starch granules. The longer the fermentation time, the more perforated or damaged the surface of the starch granules. Keywords:  Fermentation time, MOCAF flour, NIR (Near Infrared), Proximate, SEM (Scanning Electron Microscope) 
Identifikasi Senyawa Metabolit Kopi Robusta (Coffea canephora) dan Kandungan Kafein Kopi Robusta Akibat Fermentasi Bakteri Asam Laktat Lactobacillus plantarum Menggunakan UPLC-QTOF-MS/MS Dimas Andrianto; Dimas Andrianto; R. Haryo Bimo Setiarto
Jurnal Penelitian Pendidikan IPA Vol 11 No 1 (2025): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i1.9953

Abstract

Robusta coffee (Coffea canephora) is one type of plant that has long existed in Indonesia and has high economic value. Robusta coffee beans have the potential to be developed to reduce caffeine levels by treating them with lactic acid bacteria. This study aims to determine the metabolite profile of Robusta coffee from several samples treated with Lactobacilus plantarum bacteria. Samples were taken from the West Java location at the Patani Coffee Plantation. Sample extraction was carried out using the Ultrasonic Assisted Extraction (UAE) method with 96% ethanol. Metabolite content analysis was carried out using UPLC-QTOF-MS/MS with a C18 stationary phase column (Okta Decyl Silica), a mixture of formic acid/water 0.1/99.9 (v/v) and formic acid/acetonitrile 0.1/99.9 (v/v) mobile phases. The results of the analysis were interpreted using Masslynx software. The results showed that the caffeine content in coffee not fermented with Lactobacillus plantarum was 2.40%, coffee fermented with L. plantarum bacteria for 6 hours had an abundance% of 1.76%, and coffee fermented with L. plantarum bacteria for 24 hours had an abundance% of 2.38%. LC-MS/MS analysis identified compounds 35,26, and 25 in three Robusta coffee samples. These results were not significantly different from the fermentation of Lactobacillus plantarum in Robusta coffee beans