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Isolation and Characterization of Lactic Acid Bacteria From Sago Wastewater As Antibacterial Satwika Tri Rahmadana; Eka Astuty; Ony Wibriyono Angkejaya
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 13, No 1 (2024): March 2024
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jikw.v13i1.3022

Abstract

Background: Foodborne diseases are infections of the gastrointestinal tract caused by food containing microbiological agents and usually, these infections affect groups of individuals who have low immune systems. Lactic acid bacteria (LAB)are bacillus-shaped Gram-positive bacteria that work by inhibiting pathogenic bacteria that cause foodborne diseases. Maluku is a region in Indonesia rich in sago production.  Sago ihur (Metroxylon sylvestre) and tuni (Metroxylon rumphii) are types of sago commonly found in Maluku. Sago is rich in carbohydrates and therefore has the opportunity to produce LAB. This study aimed to isolate and characterize LAB from sago wastewater was a laboratory experimental research with descriptive method using primary data. Methods: Bacterial isolation was carried out using a selective media. Characterization carried out by macroscopicly characterization by observing shape, margin, elevation, pigmentation, appearance, optical properties, and texture. Microscopic characterization was performed using Gram and spore staining. Results: The results of this study showed that five bacterial isolates (I1, I2, T3, T4, and I5) were successfully isolated in a round shape, flat edges, yellowish white color (not pigmented), shiny appearance, and mucoid texture, but isolate T3 showed a raised elevation and translucent to light (transparent), while for other isolates it was convex and translucent, and non-spore forming gram positive. Conclusion: Antibacterial tests against S. aureus and E. coli showed no inhibition zones. LAB isolates from sago wastewater showed no significant antibacterial effect
Literature Study: The Effect of Butterfly Pea Flowers (Clitoria ternatea L.) on Human Health Chylen Setiyo Rini; Sukmawati Sukmawati; Ade Teti Vani; Ony Wibriyono Angkejaya; Mustar Mustar
Oshada Vol. 2 No. 1 (2025): Oshada Journal - February
Publisher : PT. Anagata Sembagi Education

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62872/1thek633

Abstract

This study aims to evaluate and summarize the findings from studies related to the effects of butterfly pea flowers (Clitoria ternatea L.) on human health, particularly those related to their therapeutic properties, including antioxidant, anti-inflammatory, neuroprotective, and relaxing effects. The research method used in this study is Systematic Literature Review (SLR). This approach was chosen because of its ability to systematically identify, evaluate, and summarize relevant research results on butterfly pea flowers. Article searches were conducted through academic databases such as PubMed, ScienceDirect, and Google Scholar, focusing on studies that discuss the effects of butterfly pea flowers on human health. Based on the analysis results of various existing studies, butterfly pea flowers show great potential in supporting human health. The content of bioactive compounds, such as flavonoids, anthocyanins, and alkaloids, provides various significant therapeutic benefits. Flavonoids and anthocyanins have strong antioxidant properties, play a role in fighting free radicals, and preventing oxidative damage that can cause degenerative diseases. In addition, these compounds have anti-inflammatory effects that are beneficial in managing inflammatory conditions such as arthritis, asthma, and heart disease. Butterfly pea flowers also show neuroprotective effects that can protect nerve cells, improve cognitive function, and protect the brain from neurodegenerative diseases such as Alzheimer's and Parkinson's. Alkaloids in butterfly pea flowers also have a relaxing effect that can help reduce anxiety and stress, and improve mental well-being. Although the results obtained are very promising, most studies are still limited to laboratory or animal trials, and further research, especially clinical trials in humans, is needed to confirm their effectiveness, proper dosage, and long-term safety.