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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA)
Abidin Nur
National Research and Innovation Agency
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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Low Temperature Preservation on Dense Biomass of Nannochloropsis oculata Indrian Rizka Amalia; Abidin Nur
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.9633

Abstract

The consistency supply of microalgae is crucial for hatchery as it plays important role as feed for larvae in the early stages. Thus, ensuring the availability of microalgae needs efficient management of feed, including the technique of biomass preservation. The purpose of this research was to observe the application of various low temperature to preserve Nannochloropsis oculata as feed. The source of N.oculata was filtered through a double set of 0.45 micron of cartridge which then preserved based on treatments A (5°C); B (-20° C); and C (15°C) for 30 days. After 30 days of preservation, each treatment as inoculum were cultured in the volume of 2 liter. Initial density of N.oculata was 23-24x106 cells.ml-1 placed into medium consisted of sterile sea water salinity 30 ppm, KW21™ fertilizer dose 1 ml.l-1. Culture environment was set with illumination 3.000-4.000 lux, homogenized with constant aeration, with 15 days of culture period. The result showed that on the days 9, cell density on treatment A, B and C were significantly different each other (sig 0.001), where treatment A had the highest cell density at 57.42±6.95x106 cell.ml-1, then followed with treatment C with 38.33±2.08x106 cell.ml-1 and B with 20.33±5.35x106 cell.ml-1. The use of low temperature as preservation for N.oculata could help low-middle hatchery with remoted area to maintain the sustainability of N.oculata stocks.
The Growth Test of Marine Microalgae Porphyridium cruentum in Different Salinity on Small Scale Culture Indrian Rizka Amalia; Abidin Nur; Lisa Ruliaty; Ratna Indria Sari
Jurnal Penelitian Pendidikan IPA Vol 9 No 11 (2023): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i11.5301

Abstract

This study aimed to know the growth response and proximate value of Porphyridium cruentum cultured in different salinities of 30 ppt, 15 ppt and 0 ppt. Culture media of 30 ppt and 15 ppt salinity were prepared with sterile seawater, while 0 ppt was from sterile aquades. Fertilizer used was KW21 (Daiichi Seimo Co., Ltd., Kumamoto, Japan) with a dose of 1 ml.l-1. All treatments were cultured for 11 days or until the cell reached exponential-stationer phase, then being growth observed and proximate analyzed. The result showed that all treatments gave the same growth pattern. Control 30 ppt had the highest cell density compare to treatments 0 ppt and 15 ppt, while 0 ppt had higher cell density to 15 ppt. The result of ANOVA (95% of significancy) showed that there was no significant different of cell density with value of 0.232. Proximate analysis showed that all treatments had high water content above 95%. The highest value of energy, carbohydrates and crude fiber were found in 0 ppt treatment, while the highest value of protein and ash were found in 30 ppt. The result from ANOVA showed that there was significant difference in proximate value with significancy less than 0.05
Co-Authors Indrian Rizka Amalia Indrian Rizka Amalia Lisa Ruliaty Ratna Indria Sari