Pudji Rahardjo
Indonesian Coffee and Cocoa Research Institute

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Viability of post acclimatized plantlets of Robusta coffee (Coffea canephora) after storage Teguh Iman Santoso; Pudji Rahardjo
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 27 No 2 (2011)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v27i2.148

Abstract

This research related to the storage method of planting materials in the form of post acclimatized plantlets of Robusta coffee multiplied by somatic embryogenesis using plastic film that wraped the whole of plantlets. This information is important to support the delivery of clonal planting materials to distribution points, especially Robusta coffee plantlets viability based on condition of the container, storage period and density of plantlets. The research was conducted at Kaliwining Experimental Station of Indonesian Coffee and Cocoa Research Institute, located at 45 m asl. D rainfall type (Schmidt—Ferguson classification). The first experiment determind the effect of container condition and storage duration on viability of Robusta coffee plantlets. Each experimental unit contained 100 plantlets and each treatment was repeated three times with completely randomized design in factorial. The first factor was condition of storage container, i.e. airtight and non air tight. The second factor was storage period levels: 0, 5, 10, 15 and 20 days. The storage container was cardboard volume 11 dm3. The second experiment was conducted for the optimization of storage volume and storage period. Each treatment using 100 plantlets was repeated three times in completly randomized design with factorial. The first factor was storage volume of 7 dm3 and 11 dm3, the second factor was storage period levels: 0, 5, 10, 15 and 20 days. The results indicated that the maximum store period was obtained in an airtight storage treatment with 10 days, 96.3% plantlets viability, 1% fallen leaves, 3.3% water loss and not significantly different to control. For packing 100 plantlets with height 8—10 cm and leaf number 4—6 can use the volume of container store up to 7 dm3, which showed no significant difference to container volume 11 dm3 in the percentage of viability, the percentage of fallen leaves, loss of water. Key words : Robusta coffee, plantlets, storage, condition, period, viability.
Pengaruh pemberian abu sekam padi sebagai bahan desikan pada penyimpanan benih terhadap daya tumbuh dan pertumbuhan bibit kakao (The effects of rice husk ash as desiccation material of seed storage on viability and cocoa seedling growth). Pudji Rahardjo
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 28 No 2 (2012)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v28i2.202

Abstract

Rice husk ash as desiccation material can be used to maintain seed viability in storage through its ability to absorb humidity during its storage. High relative humidity caused seed moisture content to increase so the respiration rate of seed increases and uses faster food stock. Finally the viability of seed is lost. A research on use of rice husk ash as desiccation material of cocoa seed storage was conducted in Agronomy Laboratory and Kaliwining Experimental Station, Indonesian Coffee and Cocoa Research Institute. Completely Randomized Design was used in this research with treatment of rice husk ash application on cocoa seed as follows: 0 g/100 seeds (A), 5 g/100 seeds (B), 10 g/100 seeds (C), 15 g/100 seeds (D), and 20 g/100 seeds (E). This experiment used four replications. Cocoa seeds were stored in plastic bag within carton box in ambient temperature. The storage periods were 1, 2, and 3 weeks, and parameters of observation consisted of electrical conductivity of dipped water of cocoa seeds, percentage of seed germination, percentage of seed emergence, early growth parameters at one month old including seedlings height of seedling, diameter, leaf number, root length, and dry weight. The result of the experiment showed that the use of rice husk ash at 5-10 g/100 seeds could maintain electrical conductivity of dipped water at low level, percentage of seed germination at 99-100 % and percentage of seed emergence at 79-91% after two weeks storage. The use of rice husk ash at 5-10 g/100 seeds after two weeks storage affected height of cacao seedling, but did not affected stem diameters, leaf numbers, root lengths, and dry weights