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Existence of entomopathogen fungi, Beauveria bassiana as an endophyte in cacao seedlings Endang Sulistyowati; Febrilia Nur AINI
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 31 No 3 (2015)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v31i3.173

Beauveria bassiana is one of the entomopathogen fungi which is known as biological control agent of cocoa pod borer and cocoa mirids (Helopeltis spp.). Because of its effectiveness in the fields is still not consistent, so we conduct a research with the objective to know the possibility of Beauveria bassiana to be established as a endophyte. Various fungal entomopathogens have already been reported as endophytes and the various methods used to inoculate the plants with B. bassiana were partially effective. The research has been conducted in laboratory of Plant Protection, Indonesian Coffee and Cocoa Research Institute by inoculating of cocoa seeds and cocoa nursery with B. bassiana suspension. The trial was arranged by randomized complete block design with a factorial arrangement. The factor were spore concentration of B. bassiana (0; 2; and 4 g/ 10 l) and cocoa varieties (family of ICS 60, TSH858, and hybrid). The trial were use four replications. The results showed that the fungal entomopathogen B. bassiana was established as an endophyte in cocoa seedling, both from cocoa seeds and nursery application. Percentage of existence of B. bassiana colonies as endophytes one month after seeds application were ICS 60 amounted to 93.3 % both on concentration treatments, while the families of TSH 858 by 80 % and 86.67 % respectively in 2 g and 4 g per 10 l of B. bassiana spores concentration treament.. The lowest percentage was in hybrids, which amounted to 66.67% and 50%. B. bassiana colonies was exixtence as an endophyte in culture from root, stem and leaves of cocoa seedling up to 5 months post inoculation. While the application on nursery by soil drenshing, leaf spraying, and stem injection , it was known that B. bassiana colonies were found in the tissues of leaves, stems, and roots until two months after application. Colonies of B. bassiana as endophytes still exsist until six weeks after nursery was planted in the field.

Effect of Pollen Feed on Parasitization and Predatism of Cephalonomia stephanoderis onHypothenemus hampei Dwi Suci Rahayu; Endang Sulistyowati
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 30 No 1 (2014)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v30i1.194

Biological control of the coffee berry borer (Hypothenemus hampei)using parasitoid Cephalonomia stephanoderishas been developed through the improvement of the parasitoid role may using pollens as feed source. The objective of this study was to investigate the effect of cover crop and weed pollens on parasitization and predatism of C. stephanoderis.The applied treatments were pollens of Turnera ulmifolia, Arachis pintoi, Ageratum conyzoidesadded in glass tube that consist of 10 CBB pupaes and a mated female of C. stephanoderis. Number of pupae parasitized and pupae preyed were observed. The result showed that addition of A. Pintoi pollen increased the number of pupae parasitized at 135% whereas addition of T. ulmifolia and A. conyzoides pollens did not affect parasitization of C. Stephanoderis. The predatismof C. stephanoderiswas higher than parasitization to pupae of H. hampei which showed that the behavior of C. stephanoderiswas parasitization. Addition of T. ulmifolia, A. pintoi, and A. conyzoidespollens increased the number of pupae predatism at 132%, 102%, and 225%, respectively. Key words: Ageratum conyzoides, Arachis pintoi, Cephalonomia stephanoderis, Hypothenemus hampei,parasitization, predatism, pollens, Turnera ulmifolia

The Effectiveness of Lemongrass, Garlic, and Tree Marigold as Botanical Insecticides in Controlling of Cocoa Mirid,Helopeltis antonii Endang Sulistyowati; Muhammad Ghorir; Suryo Wardani; Setyo Purwoko
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 30 No 1 (2014)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v30i1.195

Control of cocoa mirid, Helopeltis antoniiso far uses chemicalinsecticides as the main alternative. Therefore, it is necessary to find out the environment friendly control techniques. Lemongrass, garlic, and tree marigold have been known as an efectiveness botanical insecticides for horticulture. A research with aim to study the effectiveness of lemongrass (Cymbopogon nardus), garlic (Allium sativum) and tree marigold (Tithonia diversifolia)for controlling H. antoniihave been carried out in cocoa plantation at Kaliwining experimental garden of Indonesian Coffee and Cocoa Research Institute. The research was arranged in split plot design in three replication, with the main plot infestation time of H. antoniiand sub-plot kind of botanical insecticides. Concentration of botanical insecticides used in this study was 5% and applied on 12 cm cocoa pod in length by using knapsack sprayer. Infestation of H. antonii nymphes were conducted before and after insecticide applications. Observation was conducted on the mortality and the lesion of H. antonii. The results of orthogonal contrast test on feeding activity based on the number of lesion and percentage of mortality of H. antoniishowed that there were significantly different between insecticide treatment and control, between chemical insecticide and botanical insecticides, but there was no significant different on kind of botanical insecticides. The lowest number of lesion due to H. antonii was shown by chemical insecticide with an average 34.0, followed by garlic and lemongrass botanical insecticide with number of lesion were 51.2 and 64.7 respectively, whereas the number of lesion in the control reached 84.2. The highest percentage mortality of H. antoniiwas shown by chemical insecticide with active ingredient teta-cypermethrin at 84.3%, followed by garlic, lemon grass and tree marigold botanical insecticide were 65.8%; 65.0%; and 63.8% respectively and significantly different with control by 8,3%. Based on no significantly different on the effect of time investation of H. antoniion their feeding activity and mortality, it can be concluded that the three botanical insecticides tested have a mode of action both as a repellent and contact poison. Key words: cocoa, Helopeltis antonii, botanical insecticide, lemongrass, garlic, marigold, teta-cypermethrin

Abundance of Pollinator Insect (Forcipomyia spp .) of Cocoa Under Some Shade Trees Fakhrusy Zakariyya; Endang Sulistyowati; Dwi Suci Rahayu
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 32 No 2 (2016)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v32i2.230

Cocoa production is affected by cocoa flowering and fruiting. The factor affects fruit seeting is pollinator agent such as Forcipomyia spp. Until now, information concerning population dynamics of Forcipomyia in some models of cocoa shading trees remains limited. This research was studied to observe the population dynamics of Forcipomyia spp. in some models of cocoa shading trees, namely lamtoro (Leucaena sp.), krete (Cassia surithensis) and areca nut (Areca catechu) in two main season of rainy and dry seasons. The research was conducted in Kaliwining research station of Indonesian Coffee and Cocoa Research Institute (ICCRI), Jember, by surveying the existing cocoa under different types of shading trees as mentioned above. The insects were observed using modified sticky trap method, whilst micro climate condition was also observed. The highest population was in January (rainy season) under Leucaena spp. shade tress and the lowest population was in October under all type of the shading trees. There was no relationship between microclimate condition under cocoa trees (temperature, RH and light intensity) and Forcipomyia spp. population (r = 0.08 and 0.04).

Auto-Dissemination of Beauveria Bassiana in Controlling Coffee Berry Borer Endang Sulistyowati; Dwi Suci Rahayu; Merle Shepard; Gerry Carner
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 33 No 2 (2017)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v33i2.265

Coffee berry borer (CBB), Hypothenemus hampei Ferr. (Coleoptera, Scolytidae) is generally controlled by applying integrated pest management, particularly through sanitation and using biological agents. Beauveria bassiana is wellknown as an entomopathogenic fungus, which has been proven to be the most effective and prospective agent that could be developed to control CBB. Dissemination of microbial biocontrol agents via insect or predators may has advantages for safe spore dispersal to targeted pests with the added benefit of predation. The research with aim to determine if spores of B. bassiana can be picked up by CBB from disseminated containers had been conducted at Plant Protection Laboratory and Kaliwining Experimental Station of Indonesian Coffee and Cocoa Research Institute. Ten infected coffee berries and ten CBB adults were sprayed with various concentration of B. bassiana suspension, and then infested them in 10 healthy berries in petridish. The same trials was conducted in the field, by infested them to healthy berries on coffee branches. Observation was done on the percentage of infected berries and number of CBB infected by B. bassiana inside the berries. The results showed that spores of B. bassiana could be picked up by CBB from disseminated containers, although it was very low (2.5%). B. bassiana was not effective in suppressing CBB infestation by auto-dissemination, therefore to improve the effectiveness of B. bassiana in controlling CBB, it was advised to re-spray at least 3 times in every cropping season, especially at the flying time of CBB around 2 P.M.

Biological Control of Cocoa Pod Borer (Conopomorpha cramerella Snell) Using Egg Parasitoids of Trichogrammatoidea bactrae fumata in East Java Dwi Suci Rahayu; Endang Sulistyowati
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 34 No 1 (2018)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v34i1.307

A study on the potential of Trichogrammatoidea bactrae fumata (Tbf) parasitization in the field was conducted at an insecticide-free cocoa cultivation namely Treblasala Plantation, Banyuwangi, East Java. The research design used was a factorial randomized block design with three replications. The tested treat-ments consisted of parasitoid release intervals (once in 2 and 4 weeks) and the release population of 0 (control); 25,000 and 50,000 Tbfper hectare per release. Observations were made on number of detected eggs and parasitized eggs, and level of cocoa damage caused by cocoa pod borer (CPB) attacks. The results of this research showed that parasitization level of T. bactrae fumata on CPB eggs after3 months of the release ranged from 29.81–42.79%. The parasitoid release of CPB eggs (T. bactrae fumata) in the field for a year showed that the parasitiza-tion level of CPB eggs in the field ranged from 17.78–29.72%. Nevertheless, during that year, there was no significant effect of parasitoid release on the suppression of yield loss due to CPB attacks in the field.

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