Garuda - Garba Rujukan Digital

Pengaruh penambahan auxin terhadap pertunasan dan perakaran kopi arabika perbanyakan Somatic Embryogenesis (The effects of shooting and rooting of arabica coffee propagation through Embryogenesis Somatic auxin uses). Rina Arimarsetiowati; Fitria Ardiyani
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 28 No 2 (2012)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v28i2.201

Plantlet that has developed shoots and roots will have a high level adaptation in the field. The objective of this experiment was to improve the ability of planlet in shooting and rooting so that it is ready for acclimatization in the field. The increase ability in shooting and rooting of the planlet were conducted by adding various types of auxin in the media. The arabica coffee embryo of clone AS 2K which has entered the phase of the cotyledons was transfered into the treatment media containing half-strength of MS (Murashige & Skoog) macro and micro nutrient, vitamin B5, 30 g/L glucose, 100 ml/L coconut water, 50 mg/L AgNO3 added with the combination of IAA, IBA and NAA. The research was conducted by using completely randomized design with seven combined treatment i.e. 0.1 mg/L IBA, 0.1 mg/L NAA, 0.1 mg/L IAA; 0 , 1 mg/L IBA + 0.1 mg/L NAA, 0.1 mg/L IBA + 0.1 mg/L IAA, 0.1 mg/L NAA + 0.1 mg/L IAA; without auxin. There were 12 replications in every treatment and each replication consisted of five cotyledonary embryos. The parameters of observation were the root length, leaf number, leaf area, stem diameter, and height of plantlets. The observations were conducted in eighth weeks after cotyledonary embryo had shoots. The results showed that in the number of leaves and height of planlet parameters, the treatment without auxin was the best result compared to planlet with auxin addition. The addition of auxin varians and their combination did not significantly influent leaf area, root length and stem diameter parameters. The medium tested was optimum for the growth of shoots and roots of AS 2K arabica coffee.

Embryo Germination Development of Coffea arabica L.at Various Media Composition, Subcultures Stages and Embryo Size Rina Arimarsetiowati
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 33 No 3 (2017)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v33i3.290

Most reliable and efficient protocol for Coffea arabica L. of Sigararutang variety plant regeneration was established using embryoid as an early explant from the induction of embryogenic callus phase. A completely randomized designs with 5 replications was designed to accomplish 20 protocols of embryo germina-tion methods with different steps of subculture, size of embryo and germination medium. The embryogenic calluses from the flush leave explant were induced embryoid on a half-strength MS medium fortified with a half-strength combina-tion vitamin of 1.8 mg/L nicotinic acid, 10.1 mg/L thiamin HCl and 3.1 mg/L pyri-doxine, 50 mg/L myo inositol, 33 mg/L L.cistein, 1 mg/L Kinetin, 0.1 mg/L NAA, 20 gr/L sucrose, 2.4 gr/L gelrite and pH 5.5.The result showed that Protocol 17 was the most effective, with 59,2% of rooted cotyledons, 4.04 cm of length of roots, 1.68 cm of length of hypocotyl, 20.8% of opened cotyledons and 100% of cotyledonary embryo at the end of 8 weeks which used the B medium, large embryos and twice phase of subculture from liquid medium to solid medium. The Protocol 17 is stable protocol from low to high value. Protocol 8 is the steady protocol from high to low value. Protocols 17 and 8 are the highest and lowest ranking, respectively, for each parameter. Protocol 17 is the most suitable for the germination embryo somatic.

Leaf Mineral Composition of Coffee Infected by a Hemileia vastatrix Fungus in Bondowoso, East Java Erwin Prastowo; Novie Pranata Erdiansyah; Rina Arimarsetiowati
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 35 No 1 (2019)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v35i1.345

Leaf rust disease caused by Hemileia vastatrix fungus may become an important issue on highland coffee (Coffea arabica) especially related to the progressive increase in terms of global temperature. A research aimed at evaluating the mineral composition of some rusted coffee leaves from a single clone S 795 was carried out in Andungsari Experimental Station, Indonesian Coffee and Cocoa Research Institute. A single block experiment was situated at 1451 m asl. The intensity of rust spot in the leaves were identified, and estimated to correspond with the score of 5 to 7 out of 10. The difference in the nutrient status between normal and rusted leaves were statistically evaluated by comparing the mean values using unpaired t-test. The leaf analysis shows an optimal range for potassium (K), calcium (Ca), magnesium (Mg) and silicon (Si). Silicon distribution exhibits a high vari- ability with coefficient of variation (CV) around 30%, while K is slightly lower with CV less than 10%. Principal component analysis shows that Ca, Mg and K may have explained the most variation in the original variables as defined by PC1 (54.76%), PC2 (23.22%), and PC3 (14.01%). The rusted leaves are associated with Ca and Si while normal leaves are associated with K. A considerable high of K may explain for the depression of Ca in normal leaves which is, however, associated with an antagonism between these two minerals. The ratio of has a negative correlation with Ca, and may show a potential as an indicator for leaf mineral evaluation in the context of Hemileia vastatrix fungus infected coffee.

Characteristics of coffee as a response to different environmental conditions in Lampung: I. Morphological variations Erwin Prastowo; Rina Arimarsetiowati
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 35 No 2 (2019)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v35i2.357

The coffee growth is considered to depend, partly, on the environmental condition at which they develop to accomplish their both vegetative and generative stages. An exploratory survey aimed at investigating the morphological characteristics of 13 Robusta coffee accessions growing on different environmental conditions, i.e. altitudes, slopes, and micro-climates, in Lampung. Local superior coffee clones were selected from 8 farms to evaluate in terms of their yield components and growth characters. Three key issues are considered to observe quantitatively, i.e. plant components of leaves, branches, and fruits. The qualitative characteristics of leaves and cherries, i.e. colour and shape, were also assessed to support the comparative data analysis. The results show a similarity of ca. 60% of total qualitative variable identified among different accessions. The remaining characters are suggested to be more varying such as flush colour, leaf surface, ripe fruit colour, and stipule shape. Furthermore, a quantitative analysis shows a higher level of similarity for fruit characters, i.e. fruit length, fruit width, and fruit thickness, and to a slightly lower level for leaf characteristics, i.e. leaf length and width, and the number of productive branches. Conversely, part of yield component, such as weight of fruits, may be affected by altitude as some accessions, e.g. number 1 and 8, demonstrating a pronounced character in the principal component analysis. The same analysis reveals that some variables, i.e. fruit weight; leaf and seed; and canopy characteristics, explaining the morphological variation of coffee throughout the accessions. Furthermore, cluster analysis may indicate a possible similarity of coffee morphologies either from area with different or the same environmental conditions. Therefore, it can be concluded that environmental condition variation in Lampung may affect to a lesser extent the morphological characters of Robusta coffee.

Morphological Variations of Robusta Coffee As a Response to Different Altitude in Lampung Erwin Prastowo; Rina Arimarsetiowati
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 35 No 2 (2019)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v35i2.378

The coffee growth is considered to depend, partly, on the environmental condition at which they develop to accomplish both vegetative and generative stages. An exploratory survey in Lampung aimed at investigating the morphological characteristics of 13 Robusta coffee accessions growing on different altitudes.Local superior coffee clones were selected from eight farms, and subjected to identifications. Three key issues were concerned for both quantitatively, i.e. plant components of leaves, branches, and fruits, and qualitatively such as leaves and fruit characteristics, i.e. colour and shape. The analysis shows a similarity of about 60% of total qualitative variable identified among different accessions. The remaining characters are suggested to be more varying such as flush colour, leaf surface, ripe fruit colour, and stipule shape. Furthermore, a quantitative analysis showed a higher level of similarity for fruit characters, i.e. fruit length, -width, and -thickness, and to a slightly lower level for leaf characteristics, i.e. leaf length and -width, and number of productive branches. It was revealed that some variables, i.e. fruit weight; leaf and seed; and canopy characteristics, explaining the morphological variation of coffee throughout the accessions. Furthermore, cluster analysis may indicated a possible similarity of coffee morphologies either from area with different or the same environmental conditions. A high heterogeneity related to environmental conditions, genotypic variations, plant nutritional status, and agronomic practices, which unable to confirm in the present study, may limit the specific conclusions.

Title

Found 5 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 5 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 5 Documents
Search