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Muhammad Ridwan Harahap
Department of Chemistry, Faculty of Science and Technology, Universitas Islam Negeri Ar-Raniry, Banda Aceh
Biochemistry, Genetics & Molecular Biology Engineering

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Isolation And Identification of Bacteria In The Composting Process of Red Algae (Glacilaria sp) With The Addition of Cow Manure And Waste Household Muhammad Ridwan Harahap; Husnawati Yahya; Syafrina Sari Lubis; Sugiati Sugiati
Elkawnie: Journal of Islamic Science and Technology Vol 7, No 2 (2021)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v7i2.22536

Abstract

Abstract: Bacterial isolation research has been conducted from composting Glacilaria sp with the addition of cow dung and household waste. Glacilaria sp samples were taken from Gampoeng Neheun Pond, Mesjid Raya district, Aceh Besar. The process of composting Glacilaria sp with the addition of other materials is done with a ratio of 2:1, the composting time lasts for 20 days. Leachate sampling is carried out on days 0; 4; 8; 12; 16; and 20 as much as 10 ml. PH, color and smell measurements of the sample are performed on each leachate sampling. The observations obtained 39 Gram-negative isolates and 40 Gram-positive isolates. With morphological forms of cells namely Coccus, Bacill, Diplococcus, Streptoccus, Streptobacillus and Staphylococcus. The bacteria are most commonly found in composting household waste.Abstrak: Telah dilakukan penelitian isolasi bakteri dari pengomposan Glacilaria sp, dengan penambahan kotoran sapi dan limbah rumah tangga. Sampel Glacilaria sp berasal dari tambak Gampoeng Neheun, Kecamatan Masjid Raya Aceh Besar. Proses pengomposan Glacilaria sp dengan penambahan bahan lain dilakukan dengan perbandingan 2:1, waktu pengomposan berlangsung selama 20 hari. Pengambilan sampel lindi dilakukan pada hari ke 0, 4, 8, 12, 16, dan 20 sebanyak 10 ml. Dilakukan pengukuran pH, warna dan bau sampel pada setiap pengambilan sampel lindi. Hasil pengamatan diperoleh 39 isolat Gram negatif dan 40 isolat Gram positif. Dengan bentuk morfologi sel yaitu Coccus, Bacill, Diplococcus, Streptoccus, Streptobacillus dan Staphylococcus. Bakteri yang paling banyak ditemukan pada pengomposan limbah rumah tangga
Isolation And Identification of Bacteria In The Composting Process of Red Algae (Glacilaria sp) With The Addition of Cow Manure And Waste Household Muhammad Ridwan Harahap; Husnawati Yahya; Syafrina Sari Lubis; Sugiati Sugiati
Elkawnie Vol. 7 No. 2 (2021)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v7i2.22536

Abstract

Abstract: Bacterial isolation research has been conducted from composting Glacilaria sp with the addition of cow dung and household waste. Glacilaria sp samples were taken from Gampoeng Neheun Pond, Mesjid Raya district, Aceh Besar. The process of composting Glacilaria sp with the addition of other materials is done with a ratio of 2:1, the composting time lasts for 20 days. Leachate sampling is carried out on days 0; 4; 8; 12; 16; and 20 as much as 10 ml. PH, color and smell measurements of the sample are performed on each leachate sampling. The observations obtained 39 Gram-negative isolates and 40 Gram-positive isolates. With morphological forms of cells namely Coccus, Bacill, Diplococcus, Streptoccus, Streptobacillus and Staphylococcus. The bacteria are most commonly found in composting household waste.Abstrak: Telah dilakukan penelitian isolasi bakteri dari pengomposan Glacilaria sp, dengan penambahan kotoran sapi dan limbah rumah tangga. Sampel Glacilaria sp berasal dari tambak Gampoeng Neheun, Kecamatan Masjid Raya Aceh Besar. Proses pengomposan Glacilaria sp dengan penambahan bahan lain dilakukan dengan perbandingan 2:1, waktu pengomposan berlangsung selama 20 hari. Pengambilan sampel lindi dilakukan pada hari ke 0, 4, 8, 12, 16, dan 20 sebanyak 10 ml. Dilakukan pengukuran pH, warna dan bau sampel pada setiap pengambilan sampel lindi. Hasil pengamatan diperoleh 39 isolat Gram negatif dan 40 isolat Gram positif. Dengan bentuk morfologi sel yaitu Coccus, Bacill, Diplococcus, Streptoccus, Streptobacillus dan Staphylococcus. Bakteri yang paling banyak ditemukan pada pengomposan limbah rumah tangga
Effect of Acetobacter aceti Concentration and Fermentation Time on Acetic Acid Content Produced From Seaweed Gracilaria sp. Muhammad Ridwan Harahap; Reni Silvia Nasution; Husniah Nadhifa; Ana Khairani
Elkawnie Vol. 9 No. 1 (2023)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v9i1.26631

Abstract

Abstract: This study evaluates the conversion of Gracilaria sp. seaweed into acetic acid using the bacterium Acetobacter aceti. Acid hydrolysis breaks down the carbohydrates in Gracilaria sp. into glucose, which is then fermented by Acetobacter aceti to produce acetic acid. Assessed the impact of varying concentrations of Acetobacter aceti and different fermentation durations on acetic acid yield. Results indicated that Gracilaria sp. produced bioethanol at approximately 7.108% using bread yeast and 1.572% with tapai yeast. The bioethanol from bread yeast was used for fermentation. FTIR analysis revealed specific absorption peaks for hydroxyl (OH) at 3248 cm⁻¹, carbonyl (C=O) at 1635.64 cm⁻¹, and carbon-oxygen (C-O) at 1249 cm⁻¹. Acetic acid concentrations were influenced by the concentration of Acetobacter aceti (5%, 10%, 15%) and fermentation time (7, 10, 13 days), with the highest concentration of 0.380% achieved after 10 days at 10% Acetobacter aceti.Abstrak: Penelitian ini mengevaluasi perubahan rumput laut Gracilaria sp. menjadi asam asetat menggunakan bakteri Acetobacter aceti. Hidrolisis asam memecah karbohidrat dalam Gracilaria sp. menjadi glukosa, yang kemudian difermentasi menggunakan Acetobacter aceti untuk menghasilkan asam asetat. Pengaruh dari berbagai konsentrasi Acetobacter aceti dan durasi fermentasi yang berbeda pada hasil asam asetat. Hasil penelitian menunjukkan bahwa bioetanol Gracilaria sp. diproduksi sekitar 7,108% menggunakan ragi roti dan 1,572% dengan ragi tapai. Bioetanol dari ragi roti digunakan untuk fermentasi. Analisis FTIR mengungkapkan puncak serapan spesifik untuk hidroksil (OH) pada 3248 cm⁻¹, karbonil (C=O) pada 1635,64 cm⁻¹, dan karbon-oksigen (C-O) pada 1249 cm⁻¹. Konsentrasi asam asetat dipengaruhi oleh konsentrasi Acetobacter aceti (5%, 10%, 15%) dan lama fermentasi (7, 10, 13 hari), dengan konsentrasi tertinggi sebesar 0,380% dicapai setelah 10 hari pada 10% Acetobacter aceti.
Co-Authors Ana Khairani Husnawati Yahya Husniah Nadhifa Reni Silvia Nasution Sugiati Sugiati Syafrina Sari Lubis