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Differences in Fasting Total Cholesterol Levels in Serum and Plasma Edta Samples Using Biosystem Ba200 Aristoteles; Rosmiarti; Cindy Oktariani; Jefry Ardiansyah
Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam Vol. 21 No. 2 (2024): Sainmatika : Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam
Publisher : Universitas PGRI Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31851/sainmatika.v21i2.15850

Abstract

Examination of total cholesterol levels in serum samples is more often used as an examination material, but serum samples often encounter difficulties due to insufficient blood volume or the condition of the serum being lysed due to poor sampling. Many studies have been conducted to look at total cholesterol levels using the anticoagulants EDTA, Sodium Citrate, and Sodium Oxalate to look at differences in total cholesterol levels, but not many studies have looked at tests of differences in total cholesterol test results during fasting with serum and EDTA. This study aims to determine differences in total cholesterol levels in the fasting state of EDTA serum and plasma samples using the BA200 biosystem. The type of research used was cross sectional, which was carried out at the Hematology Laboratory of the Palembang Muhammadiyah Institute of Health Sciences and Technology and the Palembang Health Laboratory Center. A sample of 30 people was taken purposively, female, and fasting for 10 hours. Based on the results of examining total cholesterol levels in a fasting state using the Biosystem BA200, the average result for serum samples was 4.8 mmol/L and the average for plasma samples was 4.7 mmol/L. Analysis was carried out using an independent sample T-test and the result was p = 0.00, the hypothesis was accepted. This research can conclude that there is a difference between the results of examining total cholesterol levels in fasting serum and EDTA plasma samples using the BA200 biosystem.
Effect of integrated non-pharmacological therapy on second-stage labor duration and neonatal Apgar scores Rosmiarti; Marlin, Riska; Bahriah , Yuli
MEDISAINS: Jurnal Ilmiah Ilmu-Ilmu Kesehatan Vol. 24 No. 1 (2026)
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v24i1.29409

Abstract

Background: Childbirth is a physiological process influenced by maternal physical and psychological conditions. A prolonged second stage of labor is associated with increased risks of maternal fatigue, birth canal trauma, and impaired neonatal adaptation. However, evidence regarding structured integrated non-pharmacological interventions during labor remains limited. Purpose: This study aimed to evaluate the effect of an integrated non-pharmacological intervention on second-stage labor duration and neonatal Apgar scores. Methods: A quasi-experimental study was conducted from January to June 2025 among 60 women with term singleton pregnancies (intervention, n = 30; control, n = 30). Participants were allocated consecutively into two groups. The intervention consisted of Qur’anic recitation, cold compress application, and effleurage massage. The primary outcome was second-stage labor duration, and secondary outcomes were Apgar scores at 1 and 5 minutes after birth. Data were analyzed using independent t-tests and regression analysis, with a significance level of p < 0.05. Results: The intervention group had a significantly shorter second-stage labor duration than the control group (mean difference = −14.4 minutes; 95% CI: −19.2 to −9.6; p < 0.001). Neonates in the intervention group had significantly higher Apgar scores at 1 minute (mean difference = 0.8; 95% CI: 0.41 to 1.19; p = 0.002) and 5 minutes (mean difference = 0.6; 95% CI: 0.32 to 0.88; p = 0.001). Conclusions: An integrated non-pharmacological intervention was associated with shorter second-stage labor duration and higher early neonatal Apgar scores. This multimodal approach may provide a feasible, low-cost supportive strategy for intrapartum care in low-resource settings. Further randomized controlled trials are warranted to confirm these findings.