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Maftuch
University of Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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Evaluation of the Effectiveness of Organic Matter Biodegradation by a Bacterial Consortium in Vannamei Shrimp Farming Wastewater A. Fahmi Nada; Surya Rachman Susilowati; Asus Maizar Suryanto Hertika; Maftuch
Jurnal Penelitian Pendidikan IPA Vol 11 No 2 (2025): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i2.9983

Abstract

This study evaluated the effectiveness of a bacterial consortium for biodegrading organic waste in vaname shrimp (Litopenaeus vannamei) farming wastewater. A completely randomized design with four treatments (1, 2, and 3 ppm, plus a control) and three replicates per treatment was used. The consortium, consisting of Lactobacillus casei, Bacillus subtilis, Bifidobacterium sp., Nitrosomonas sp., and Nitrobacter sp., was cultured in a medium containing molasses, rice bran, brown sugar, and whole milk. Over a 5-day incubation period, key parameters (TOM, BOD, ammonia, nitrite, nitrate, and bacterial viability) were measured. The 3 ppm treatment achieved the highest reductions TOM by 61.4%, BOD by 69.57%, ammonia by 68.92%, nitrite by 67.59%, and nitrate by 62.02% with bacterial viability increasing by 165% compared to the control. These results demonstrate that the optimal dose significantly enhances biodegradation under conditions of 24.3–28.9°C, pH 7.8–8.5, and DO 3.5–6.8 mg/L. In conclusion, applying the bacterial consortium at 3 ppm offers an eco-friendly and effective strategy to improve water quality in intensive shrimp aquaculture, supporting national food security and Blue Economy initiatives.
Identifikasi dan Analisis Molekuler Hemosianin sebagai Gen Ketahanan Tubuh Udang Vaname (Litopenaeus vannamei) Talitha Amanda; Yuni Kilawati; Maftuch
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.7331

Abstract

Production of white shrimp (Litopenaeus vannamei) tends to decrease every year caused by diseases, one of which is White Feces Disease (WFD). Genetically, monitoring has been carried out on the parent characters of SPF (Specific Pathogen Free) shrimp imported from Hawaii and Florida with low genetic variation. If there is a threat such as disease caused by bacteria and the gene that codes for disease control or the body's resistance gene is missing, it allows the individual to become susceptible to disease. Gene that codes for body resistance in white shrimp is hemocyanin. Hemocyanin gene studies are needed as a first step in controlling disease in white shrimp. Method used is a descriptive method. Procedures carried out are sample collection, detection of WFD, molecular analysis of hemocyanin and data analysis. Results showed that healthy shrimp had hemocyanin while shrimp with WFD had very little or no amount of hemocyanin. Hemocyanin gene profile in healthy shrimp was also analyzed and there were 4 nucleotide change made changes to 4 amino acid. Changes in amino acids affect the stability of hemocyanin and cause polymorphism of hemocyanin. Phylogenetic analysis showed dendogram split into two clades between L. vannamei and P. monodon.
Co-Authors A. Fahmi Nada Asus Maizar Suryanto Hertika Surya Rachman Susilowati Talitha Amanda Yuni Kilawati