Maria Ulfah
Departemen Ilmu Produksi dan Teknologi Peternakan, Fakultas Peternakan, IPB University, Kampus IPB Darmaga, Bogor 16680

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Avian Sex Determination Based on Chromo Helicase DNA-binding (CHD) Genes Using Polymerase Chain Reaction (PCR) I Khaerunnisa; E Sari; M Ulfah; . Jakaria; C Sumantri
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (468.753 KB) | DOI: 10.5398/medpet.2013.36.2.85

Abstract

Several bird species are sexually monomorphic. In this case, molecular approach is an efficient method for their sex determination. The sexes of monomorphic birds can be determined by PCR amplification of the CHD genes. CHD genes are preserved within avian Z and W sex chromosomes. The objective of this research was to determine sex of 21 individuals quails, pigeons, hill myna, salmon-crested cockatoos, and yellow-crested cockatoos based on CHD genes using two pairs of primers: 2550F-2718R and P2-P8. Samples from Kampong chickens and ducks were used as controls. PCR products were screened by agarose gel electrophoresis with ethidium bromide. Results showed that 2550F and 2718R primers could be used to identify sex of Kampong chickens, quails, ducks, pigeons, hill myna, salmon-crested cockatoos, and yellow-crested cockatoos. Individuals showed double (ZW) and single (ZZ) bands were identified as females and males, respectively in Kampong chickens, quails, hill myna, salmon-crested cockatoos and yellow-crested cockatoos. Males and females in ducks and pigeons showed single band in different length of base pairs. P2 and P8 primers could be used to identify the sex of pigeons, hill myna, salmon-crested cockatoos, and yellow-crested cockatoos. All of those samples showed bouble (ZW) bands for females and single (ZZ) band for males.
Eksplorasi Preferensi Masyarakat Terhadap Pemanfaatan Ayam Lokal di Kabupaten Bogor Jawa Barat Arma Aditya Kartika; Kanthi Arum Widayati; . Burhanuddin; Maria Ulfah; Achmad Farajallah
Jurnal Ilmu Pertanian Indonesia Vol. 21 No. 3 (2016): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (688.694 KB) | DOI: 10.18343/jipi.21.3.180

Abstract

Indonesian native chickens consist of indigenous chickens and a chickens originated from abroad which adapted and grow in Indonesia. Based on 120 respondents who have been interviewed, there were seven types chicken commonly raised, namely: kampong, bangkok, arab, kate, pelung, gaga’, and birma. People commonly raised native chickens for religion, food, ornamental, and trading purposes. Six of those seven native chickens (86%) are reared for trading, five types (71%) for ornamental, and four types (57%) for egg and meat production purpose. Three types chicken (43%) were well-known had the benefits for eggs production. The religious category was the highest ICF value (1.00) followed by food (meat and eggs production) category with ICF value by 0.98. Arab chicken had the highest Fidelity Level (FL) value (70.96%) as egg and meat producer. Egg production of arab chicken reached 22.50 egg/period and it was the highest productivity of all experimental chickens. Average flock size per household was 11.31 with a population around 225 chickens/household. Kampong chicken was the most popular to be raised (79.16%) followed by bangkok (40.00%), kate (13.33%), gaga’ (10.00%), arab (8.33%), pelung (5.83%), and birma (3.33%). Kampong chicken is a food source and highly used for religion purpose had 38.23% of FL value
POTENSI TUMBUHAN OBAT SEBAGAI FITOBIOTIK MULTI FUNGSI UNTUK MENINGKATKAN PENAMPILAN DAN KESEHATAN SATWA DI PENANGKARAN Maria Ulfah
Media Konservasi Vol 11 No 3 (2006): Media Konservasi
Publisher : Department of Forest Resources Conservation and Ecotourism - IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.482 KB) | DOI: 10.29244/medkon.11.3.%p

Abstract

Managing the feeding program is the most important concept in feeding of wild animals in captivity to maintain their performance, health condition and absolutely sustainability. Since the use of medicine plants show significantly improvement of the performance and health condition of domesticated animals, it sounds promisingly to use such plants for wild animals in captivity, especially for commercial purposes. Due to their properties, the medicinal plants can be classified as appetite stimulating substances, digestive enhancers, bacterial steering agents, metabolic modifiers and odour neutralizing agents, that is called multi-function phytobiotics (MFP). However further studies should be done to investigate an effectiveness-oriented of active components of medicinal plants on the regulation of performance and health condition of wild animals in captivity.Keywords : Feeding program, wild animal, medicinal plant, Multi Function Phytobiotic, performance, health
KEANEKARAGAMAN SATWA BERKHASIAT OBAT DI TAMAN NASIONAL BETUNG KERIHUN, KALIMANTAN BARAT INDONESIA (Diversity of Medicinal Animals in Betung Kerihun National Park, West Kalimantan, Indonesia) Yohanes AE Putra; Burhanuddin Masy'ud; Maria Ulfah
Media Konservasi Vol 13 No 1 (2008): Media Konservasi
Publisher : Department of Forest Resources Conservation and Ecotourism - IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.842 KB) | DOI: 10.29244/medkon.13.1.%p

Abstract

This study was carried out to gain data on animal species and their subtances/derivatives believed had medicinal properties that were utilized and traded by local people around Betung Kerihun National Park (BKNP). The study was conducted at the 12 villages around area of BKNP. Snowball method was used to determine respondents. There were 32 respondens interviewed. The data obtained was analysed by using descriptive quantitatively method. The study showed that there were 39 animal species and 19 animal substances/derivatives that utilized as medicinal purposes by local people around BKNP. There were also 9 animal species and 4 animal subtances/derivatives traded by those peoples. Local peopl’s perspectives on animal utilization had been changed from nature to economic orientation. Law enforcement, giving conservation education, involving local peoples on biodiversity conservation management, and introducing captive breeding program to local communities are needed to raise the understanding and awareness of the importance of habitat and animal species conservation in BKNP.Key word: Medicinal Animal, subtances/derivatives, medicinal purposes, utilization, trade, conservation.
PRODUCTION PERFORMANCE EVALUATION OF PRE-LAYING PERIOD AT 3 STRAINS OF GPS BROILER CHICKEN IN INDONESIA Bestari, Ahmad; Darwati, Sri; Ulfah, Maria; Muladno, Muladno
Jurnal Ilmu Ternak Universitas Padjadjaran Vol 23, No 2 (2023): December
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v23i2.47567

Abstract

The production performance of GPS on Cobb, Indian River, and Hubbard strains has not been well studied. This study aimed to compare the production performance between female GPS strains at 24 weeks of age and the standards set by each principal. This research was conducted from November 2022 to March 2023. The results showed a significant difference in most production performance between GPS strains at 24 weeks of age by each strain. Significant differences were observed for body weight, feed consumption, depletion and uniformity. As for comparing the production performance between the GPS strains and the standards set by the principal, there were also significant differences, and several performance parameters exceeded the standards. This result was influenced by different farming management patterns from each company adapting to actual conditions in the field.
PERFORMANCE OF FOUR STRAINS OF BROILER CHICKEN GRANDPARENT STOCK FLOCK IN INDONESIA Rofii, Rofii; Ulfah, Maria; Darwati, Sri; Muladno, Muladno
Jurnal Ilmu Ternak Universitas Padjadjaran Vol 23, No 2 (2023): December
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v23i2.47564

Abstract

This study aims to compare the performance of broiler GPS flocks from 4 GPS strains in Indonesia (Cobb, Ross, Indian River, Hubbard). This research was conducted from November 2022 to March 2023. Data were collected from some breeding companies' audit results conducted by the Directorate General of Livestock and Animal Health. The results showed that the broiler GPS flock performance in the young age group showed a significance value of <0.05, and there was a significant difference in the HDP variable indicator. The average HDP weight for the Ross, Cobb, Indian River, and Hubbard GPS strains was 78.05±3.28, 74.00±3.30, 78.99±3.37 and 75.85±3.61, respectively. The average egg and hatchling weight was also significantly different among each strain, but only on the variable factor of the breeding company. There were no significant differences among the strain factor variables and primary product variables, such as the average number of DOC PS per hen by breeding company and strain. On average, a female GPS line in the young age group period for the Ross, Cobb, Indian River, and Hubbard strains produced 15.14 ± 2.2, 14.92 ± 4.6, 15.35 ±1.47, and 13.78 DOC, respectively. There is no mutual interaction between the performance of HDP flocks, egg weight, and hatching weight of DOC PS from variable factors of the breeding companies with each strain. Factorial interactions only occur in the primary product variable, meaning each broiler GPS strain reared by each breeder company in Indonesia has different performances.
Genetic Structure of Gallus varius Based on Middle-Lower Section of Control Region mtDNA Achmad, Alfiyan; Farajallah, Achmad; Ulfah, Maria; Perwitasari-Farajallah, Dyah; Muladno, Muladno
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.548-558

Abstract

Gallus varius, an indigenous bird species of Indonesia, demonstrates significant genetic diversity. The genetic diversity assessment in G. varius encompassed both mitochondrial DNA (mtDNA) and nuclear DNA, with the mtDNA analysis primarily centered on domain I of the control region. This study aimed to describe the genetic diversity and structure of G. varius inhabiting Java, Madura, Lombok and Sumbawa based on the middle-lower part of control region mtDNA. Genomic DNA was extracted from the calamus tip of feather, then the middle-lower part of control region was amplified and sequenced using two pairs of primers. In the examined control region, spanning from 944 to 1,008 bp, 13 bp of nucleotide variation was observed, with nucleotide diversity at 0.0021. Between G. varius samples and the reference (NC_007238.1), a total of 7 haplotypes were identified, 5 unique and 2 shared haplotypes, with haplotype diversity at 0.7692. The substantial diversity of haplotypes in this study and two previous study suggests that the genetic diversity of G. varius has remained stable over the past two decades. Additionally, genetic distance data indicate there is multiple G. varius subspecies, and the haplotype network accentuates signs of population differentiation.
Characterization of CDS Region of Exons 1 and 2 of SOX9 Gene as Potential Gene in Construction of Syrinx Structure in Junglefowl (Gallus sp.) Alfiyan, Achmad; Farajallah, Achmad; Maria, Ulfah; Perwitasari-Farajallah, Dyah; Muladno, Muladno
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1130-1143

Abstract

The crowing of male Gallus exhibits diverse sound patterns. This is believed to be related to the phenotypic diversity of vocal organs, one of which is influenced by the nucleotide diversity of the associated genes. The SOX9 gene, involved in cartilaginous tissue growth and development, is reported to contribute e in the development of larynx and syrinx. This study aimed to characterize the CDS regions of exons 1 and 2 of the SOX9 gene in junglefowl to assess its diversity. Genomic DNA was extracted from ten individuals of G. varius from Lombok and Sumbawa. The CDS regions of SOX9 gene exons 1 and 2 were amplified using two primer pairs. Additionally, the CDS regions of SOX9 gene exons 1 and 2 from 54 junglefowl SRA data in an online repository were mapped and analyzed. The study identified all nucleotide sequences as CDS regions of SOX9 gene exons 1 and 2. Six shared, and 24 unique haplotypes were constructed. A putative amino acid sequence common to all Gallus species was also identified. The diversity observed in the CDS regions of SOX9 gene exons 1 and 2 nucleotide sequence showed a different level with the diversity observed in its amino acid sequence.
The Quality of Hatching Egg and Development of Embryonic Somites in Kampung and Sentul Chickens Zulfikar, Muhammad Irsyad; Ulfah, Maria; Fahrudin, Mokhamad
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 3 (2024): December 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.03.14

Abstract

Kampung and Sentul chickens are Indonesian local chicken breeds that are widely developed by breeding companies as strains for meat production. The genetic differences among these chicken strains can impact the quality of the hatching eggs they produce. Somite development is a crucial part of chicken embryo growth during the hatching process, as it precedes the formation of vertebrae and skeletal muscles, ultimately affecting chick quality. This study aimed to analyse how both strains of hens affect hatching egg quality and somite development. The sample included 126 hatching eggs, with 63 from Kampung chickens and 63 from Sentul chickens. The observed parameters of hatching egg quality included egg weight, egg shape index, eggshell thickness, eggshell weight, eggshell strength, albumen index, yolk index, yolk color score, Haugh Unit (HU), and embryo development indicators, such as the development of the primitive streak and progression of somite number. The data were analysed using a t test. Differences between chicken strains significantly affected (P<0.05) egg weight, shell thickness, the albumen index, and HU. The different strains also showed significant differences (P<0.05) in embryo somite development, with the primitive streak development of the Sentul chicken being slower than that of the Kampung chicken. In conclusion, Kampung chickens produce hatching eggs with superior interior quality, primitive streak development and somite number compared to the hatching eggs of other chickens. However, Sentul chickens also demonstrate good exterior egg quality, as shown by their egg weight and shell thickness.