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All Journal Media Farmasi Indonesia Antigen: Jurnal Kesehatan Masyarakat dan Ilmu Gizi
Elisa Rinihapsari
Politeknik Katolik Mangunwijaya
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Other

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Comparison of the Antibacterial Activity of Orange Peel Eco-Enzyme against Staphylococcus aureus and Escherichia coli Elisa Rinihapsari; Materius Kristiyanto; Zalsa Billa Lutia Tambora
Media Farmasi Indonesia Vol. 21 No. 1 (2026): Media Farmasi Indonesia
Publisher : SEKOLAH TINGGI ILMU FARMASI YAYASAN PHARMASI SEMARANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53359/mfi.v21i1.359

Abstract

Orange peel contains various bioactive compounds known to possess antibacterial and antioxidant activities, making it a potential raw material for eco-enzyme production. This study aimed to determine the concentration of orange peel eco-enzyme that exhibits the highest antibacterial activity against Staphylococcus aureus and Escherichia coli, as well as to compare the antibacterial activity of orange peel eco-enzyme against Staphylococcus aureus and Escherichia coli. Another objective was to evaluate differences in antibacterial activity at different incubation times. This study employed an experimental design, involving antibacterial activity testing of orange peel eco-enzyme using the Kirby–Bauer disk diffusion method. The independent variable was the concentration of orange peel eco-enzyme at 70%, 80%, 90%, and 100%. Other independent variables included bacterial species and incubation times of 18 and 24 hours. The overall statistical analysis showed that the eco-enzyme exhibited significant antibacterial activity against both Staphylococcus aureus and Escherichia coli, with the optimal concentration being 90%. The Mann–Whitney test indicated no significant difference in antibacterial activity between Staphylococcus aureus and Escherichia coli (p > 0.05). Comparison between incubation periods of 18 and 24 hours also revealed no significant difference in antibacterial activity (p > 0.05), indicating that the orange peel eco-enzyme has good stability of antibacterial activity against both test bacteria.
Pengaruh Pemanasan Berulang Media Nutrient Agar Terhadap Hasil Uji Sensitivitas Elisa Rinihapsari; Benaya Yamin Onesiforus; Samuel Marchel Nugroho
Antigen : Jurnal Kesehatan Masyarakat dan Ilmu Gizi Vol. 1 No. 4 (2023): November : Antigen: Jurnal Kesehatan Masyarakat dan Ilmu Gizi
Publisher : LPPM STIKES KESETIAKAWANAN SOSIAL INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/antigen.v1i3.191

Abstract

Nutrient agar media is a universal medium that can be used in bacterial sensitivity tests. Sensitivity testing is used to test the sensitivity of bacteria to a type of antibiotic. This test is instrumental in determining appropriate therapy for patients who experience recurrent or persistent infectious diseases. Nutrient Agar media is usually made in large quantities for time and energy efficiency. The media is used as needed, and the rest is stored 2-8oC in the refrigerator before being used again. This research aims to determine the effect of repeated heating of Nutrient Agar media on the sensitivity test results for Staphylococcus aureus and Escherichia coli bacteria. Tests were carried out on the first, second, third and fourth heating times. The results of statistical tests with Kruskal Wallis showed a sig value of <0.05, meaning there was a difference in sensitivity test results between media heated once, twice, three times, or four times for the two types of bacteria tested.
Co-Authors Benaya Yamin Onesiforus Materius Kristiyanto Samuel Marchel Nugroho Zalsa Billa Lutia Tambora