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All Journal Jurnal Analis Medika Biosains (JAMBS)
Alya Salsabila
Universitas Nahdlatul Ulama Surabaya
Biochemistry, Genetics & Molecular Biology Health Professions

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Differences In Storage Time In Safranin Dye On Counting The Number Of Platbocytes By Rees Ecker Method Alya Salsabila; Gilang Nugraha
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.347

Abstract

Platelet count examination is important to assess hemostasis function. One of the commonly used manual methods is the Rees Ecker method. However, during the examination, impurities were found that were difficult to distinguish from platelets. Therefore, this study aimed to determine the difference in platelet count results using the Rees Ecker method against storage time with safranin dye. This study used an experimental method on 10 normal blood samples. Platelets were counted with an Improved Neubeauer hemocytometer using safranin dye stored for 0 days, 1 day, 7 days, 14 days, and then compared with the platelet count results using Rees Ecker as a control solution. The Mann Whitney Test results showed no significant difference between the control group and the safranin group in terms of platelet count at 0 days (sig. 0.850), 1 day (sig. 0.820), 7 days (sig. 0.130), and 14 days (sig. 0.121) of storage. Safranin is safe and effective for counting platelets using the Rees Ecker method, as shown by the stability test results for 14 days.
Co-Authors Gilang Nugraha