Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal Makara Journal of Science
Yokota, Akira
Unknown Affiliation

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Cultivation of Uncultured Bacteria of the Class Ktedonobacteria in the Phylum Chloroflexi Yokota, Akira
Makara Journal of Science Vol. 16, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The class Ktedonobacteria in the phylum Chloroflexi is known to contain a large number of uncultured, environmental 16S rRNA gene clones, and cultured representatives are a limited number. In this review, recent findings on the taxonomical and ecological significance of the class Ktedonobacteria in the phylum Chloroflexi are discussed based on the findings from both the characteristics of the cultured Ktedonobacteria and molecular-based analysis. The microorganisms characterized so far include four species in three genera, Ktedonobacter, Thermosporothrix and Thermogemmatispora, and were proposed to represent three families, Ktedonobacteraceae, Thermosporotricaceae, and Thermogemmatisporaceae, and two orders, Ktedonobacterales and Thermogemmatisporales. Ktedonobacteria strains showed a common property of gram-positive, aerobic organisms that produce branched vegetative mycelia and form spores by budding.
Activities of Cellulolytic, Amylolytic, and Secondary Metabolites from Thermophilic Actinomycetes Collected from the Heated Soil around Cisolok Geyser Sari, Dhian Chitra Ayu Fitria; Ningsih, Fitria; Rachmania, Mazytha Kinanti; Yokota, Akira; Sjamsuridzal, Wellyzar
Makara Journal of Science
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study investigated the antimicrobial activity, cellulolytic, and amylolytic abilities of eight thermophilic Actinomy-cetes isolated from the heated soil around the Cisolok geyser, West Java, Indonesia. Cellulolytic and amylolytic abili-ties were screened for on media by adding 1% (w/v) soluble starch and carboxymethylcellulose (CMC), respectively. Antimicrobial activity was screened for using the agar-plug diffusion method by cultivating strains on various growth media. Growth at various temperatures showed that six strains could grow up to 50 °C. Seven out of eight strains showed amylolytic ability at 40 °C and 45 °C, and only four strains did so at 50 °C. Screening for cellulolytic ability showed that seven strains could hydrolyze CMC at 40 °C, whereas seven and five strains could hydrolyze at 45 °C and 50 °C, respectively. Antibacterial activity showed that strains UICC B-110 and UICC B-118 grown on various media could inhibit the growth of Gram-positive bacteria. The results of the antifungal activity test showed that strain UICC B-118, incubated for seven and 14 days, could inhibit the growth of Candida albicans. Based on 16S rRNA gene se-quence similarity and phylogenetic analyses, strains UICC B-110 and UICC B-118 were identified as Streptomyces glaucescens and Microbispora rosea subsp. rosea, respectively. These results show that thermophilic Actinomycetes from the soil around the Cisolok geyser have the potential as a source of thermostable enzymes and new bioactive compounds with antimicrobial activity.
Co-Authors fitria ningsih Rachmania, Mazytha Kinanti Sari, Dhian Chitra Ayu Fitria WELLYZAR SJAMSURIDZAL