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All Journal Journal Of Vocational Health Studies Jurnal Paradigma: Pemberdayaan & Pengabdian Kepada Masyarakat
Yogi Khoirul Abror
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Health Professions Medicine & Pharmacology Nursing Public Health

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PEMERIKSAAN KADAR HB DAN PENYULUHAN TENTANG ANEMIA SERTA ANTISIPASINYA PADA SISWA SMA AL HIDAYAH Hamimatus Zainiyah; Yogi Khoirul Abror
JURNAL PARADIGMA (PEMBERDAYAAN & PENGABDIAN KEPADA MASYARAKAT) Vol 1 No 2 (2019): JURNAL PARADIGMA VOLUME 1 NOMOR 2 OKTOBER 2019
Publisher : STIKES NGUDIA HUSADA MADURA

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Abstract

Anemia is a condition in which the components in the blood, namely hemoglobin (Hb) in the blood, are less than normal levels. Determination of anemia can also be done by measuring the hematocrit (Ht), which on average is equivalent to three times the hemoglobin level (Tarwoto, 2010). Based on the World Health Organization (WHO) 2010 states that of all adolescents in developing countries the prevalence of anemia in adolescent girls is 53.7%. The results of the Basic Health Research (RISKESDAS) in 2013, showed that the prevalence of anemia in Indonesia was 21.7%. Anemia patients aged 5-14 years were 26.4% and patients aged 15-24 years were 18.4% (Kemenkes RI, 2014). Based on a survey with interviews with 10 female students, 7 students experienced weakness, fatigue and lack of concentration on learning during menstruation and on physical examination showed pale conjunctiva. This is due to poor diet and insufficient iron intake. The method used to carry out community service is by conducting a preliminary study conducted at Al Hidayah High School, then conducting interviews about the understanding of anemia and how to anticipate it then making a time contract with students. The activity was continued with counseling and training in preparing a balanced menu, then continued with anamnese and examination of HB levels carried out at Al Hidayah High School. Of the 120 students, only 24 students showed signs and symptoms of anemia. Only students who showed signs and symptoms of anemia were tested for HB levels. While participating in the activities the students were very enthusiastic about the material presented, the students were also able to demonstrate compiling a balanced menu. The results of early detection of 24 students showed that 54.2% were not anemic and 45.8% had anemia.
OPTIMIZATION OF DENATURATION TEMPERATURE AND TIME USING REAL-TIME PCR METHOD IN HEPATITIS B TEST Tanti Yulia Dewi; Fusvita Merdekawati; Ai Djuminar; Yogi Khoirul Abror
Journal of Vocational Health Studies Vol. 8 No. 2 (2024): November 2024 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V8.I2.2024.131-136

Abstract

Background: The hepatitis B test can be conducted using the real-time PCR method. In chronic cases, the test is performed by detecting one of the Hepatitis B Virus (HBV) specific genes, HBcAg. The real-time PCR method requires optimization to obtain optimal results. Purpose: This research aims to determine the optimal temperature and time for denaturation in the hepatitis B test using the real-time PCR method. Method: The research method used was a quasi-experiment involving variations in temperature (94 °C, 95 °C, and 96 °C) and time (10 seconds, 15 seconds, and 20 seconds) for denaturation. Data processing resulted in static group comparisons based on 27 primary data from the Cycle Threshold (CT) value. Result: Variations in temperature conditions, specifically at 94 °C, 95 °C, and 96 °C, combined with a denaturation time of 10 seconds, yielded the mean CT values of 26.495, 26.355, and 26.003, respectively. When the temperature conditions were maintained at 94 °C, 95 °C, and 96 °C, with a denaturation time of 15 seconds, yielded the mean CT values of 25.962, 25.641, and 25.396. Similarly, under temperature conditions of 94 °C, 95 °C, and 96 °C with a denaturation time of 20 seconds, yielded the mean CT values of 26.544, 26.505, and 25.830 were obtained. Conclusion: Optimal results in this study are obtained through the acquisition of the smallest CT value, namely at temperature conditions of 96 °C and 15 seconds.
Co-Authors Ai Djuminar Fusvita Merdekawati Tanti Yulia Dewi Zainiyah, Hamimatus