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IbM Pembuatan Keripik Jamur Tiram Dengan Teknologi Vakum Di Desa Sidosari, Lampung Selatan Lilis Hermida; Joni Agustian; Yunita Kesuma
Jurnal Pengabdian Kepada Masyarakat Sakai Sambayan Vol 1 No 3 (2017): Jurnal Sakai Sambayan
Publisher : Lembaga Penelitian dan Pengabdian Universitas Lampung

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Abstrak —Permasalahan utama yang dihadapi oleh petani jamur di desa Sidosari dan desa Sinar Harapan propinsi Lampung adalah sulitnya penanganan paska panen jamur tiram karena jamur tiram mudah rusak dan cepat membusuk. Sehingga harga jamur tersebut relatif rendah dan jangkauan pemasarannya terbatas, terutama pada musim hujan. Adapun solusi yang ditawarkan adalah sosialisasi/demonstasi pengolahan jamur tiram menjadi keripik jamur tiram dengan menggunakan mesin vacuum frying dan sosialisasi desain kemasan yang baik. Teknologi vakum dioperasikan pada suhu dan tekanan yang lebih rendah dibandingkan dengan penggorengan biasa sehingga keripik jamur tiram yang dihasilkan lebih renyah dan lebih bernutrisi. Sebelum sosialisasi, serangkaian percobaan dilakukan untuk mengetahui kondisi operasi terbaik mesin vacuum frying untuk menggoreng 1 kg jamur tiram segar. Pada percobaan tersebut, mesin vacuum frying dijalankan pada suhu 70 dan 80 oC sementara waktu penggorengan yang diamati adalah dari 20 hingga 50 menit. Dari hasil percobaan tersebut diketahui bahwa suhu 80 oC dan waktu penggorengan 40 menit adalah kondisi operasi yang terbaik mesin vacuum frying untuk menghasilkan keripik jamur. Setelah sosialisasi/demonstrasi penggunaan mesin vacuum frying tersebut, petani jamur berhasil menggoreng 1 kg jamur tiram segar dengan pendampingan. Kata kunci — Keripik jamur tiram, Desa Sidosari, Vacuum frying Abstract — The main problem faced by oyster mushroom farmers in Sidosari village and Sinar Harapan village in Lampung province was the difficulty of post-harvest handling of fresh oyster mushrooms because they are easily damaged and quickly decay. As such, the mushroom price was relatively low and its marketing scope was limited, especially in rainy seasons. The solution offered were the socialization/demonstration of processing of fresh oyster mushroom into oyster mushroom crisps by using a vacuum frying machine and socialization of a good packing design. The vacuum technology was operated at lower temperature and pressure compared to use conventional atmospheric frying so that oyster mushroom crisps produced were more crispiness and nutritious. Before the socialiation was held, several experiments were carried out to obtain the best operation condition of vacuum frying machine to fry 1 kg of fresh oyster mushrooms. In the experiments, vacuum frying was run at 70 and 80 oC, while the frying times observed were from 20 to 50 minutes. It was found that temperature of 80 oC and frying time of 40 minutes were the best conditions of the vacuum frying to produce mushroom crisps. After the socialization/demonstration of the vacuum frying uses, the farmers successfully fried 1 kg of raw oyster mushrooms under guidance. Keywords—— Oyster mushroom crisps, Sidosari village, Vacuum frying

Optimasi Proses Hidrolisis Pati Tapioka Menggunakan Glukoamilase Terimobilisasi pada Silika MCF 9.2T-3D Berdasarkan Response Surface Methodology (Box-Behnken Design) Joni Agustian; Lilis Hermida; Pia Sabrina Murtadho
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2018: PROSIDING SNTKK 2018
Publisher : Seminar Nasional Teknik Kimia Kejuangan

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Central Composite Statistical Design of Tapioca Starch Hydrolysis using Immobilized Glucoamylase on Mesostructured Cellular Foam Silica (MCF-9.2T-3D) Joni Agustian; Lilis Hermida; Ade Febriana Syahfitri
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2018: PROSIDING SNTKK 2018
Publisher : Seminar Nasional Teknik Kimia Kejuangan

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The Optimised Statistical Model for Enzymatic Hydrolysis of Tapioca by Glucoamylase Immobilised on Mesostructured Cellular Foam Silica Joni Agustian; Lilis Hermida
Bulletin of Chemical Reaction Engineering & Catalysis 2019: BCREC Volume 14 Issue 2 Year 2019 (August 2019)
Publisher : Department of Chemical Engineering - Diponegoro University

Enzymatic hydrolysis of starches using free glucoamylase to reducing sugars have difficulties in recovering and recycling of the enzyme, hence immobilisation on inert supports were widely studied. However, effectiveness of the immobilised glucoamylase were merely observed only on soluble starches. It was considered a valuable thing to know performance of glucoamylase on Mesostructured Cellular Foam (MCF) silica in hydrolysing of tapioca. An optimised study on enzymatic hydrolysis of tapioca using glucoamylase on MCF silica (9.2T-3D) and its kinetics were described including justification of the predicted model as it was required to develop in large scale operations. Central Composite Design was used to model the process by studying effects of three factors on DE values after enzyme immobilisation. Immobilisation of glucoamylase on this support gave up to 82% efficiency with the specific activity of 1,856.78 U.g-1. Its used to hydrolysis of tapioca resulted DE values of 1.740-76.303% (w/w) where the highest DE was obtained at pH of 4.1, temperature of 70 ℃ and agitation speed of 140 rpm. The optimisation produced a polynomial quadratic model having insignificant lack-of-fit and low standard deviation, so that it was applicable and reliable in simulating the DE with only 0.80% of data were not described. Temperature affected the process highly, but the buffer pH, agitation speed and factorial interactions were considered not important. KM value for immobilised enzyme was better than the free glucoamylase, however, its reaction rate was slower than the free glucoamylase catalysis. Copyright © 2019 BCREC Group. All rights reserved

The Optimised Statistical Model for Enzymatic Hydrolysis of Tapioca by Glucoamylase Immobilised on Mesostructured Cellular Foam Silica Joni Agustian; Lilis Hermida
Bulletin of Chemical Reaction Engineering & Catalysis 2019: BCREC Volume 14 Issue 2 Year 2019 (August 2019)
Publisher : Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.9767/bcrec.14.2.3078.380-390

Enzymatic hydrolysis of starches using free glucoamylase to reducing sugars have difficulties in recovering and recycling of the enzyme, hence immobilisation on inert supports were widely studied. However, effectiveness of the immobilised glucoamylase were merely observed only on soluble starches. It was considered a valuable thing to know performance of glucoamylase on Mesostructured Cellular Foam (MCF) silica in hydrolysing of tapioca. An optimised study on enzymatic hydrolysis of tapioca using glucoamylase on MCF silica (9.2T-3D) and its kinetics were described including justification of the predicted model as it was required to develop in large scale operations. Central Composite Design was used to model the process by studying effects of three factors on DE values after enzyme immobilisation. Immobilisation of glucoamylase on this support gave up to 82% efficiency with the specific activity of 1,856.78 U.g-1. Its used to hydrolysis of tapioca resulted DE values of 1.740-76.303% (w/w) where the highest DE was obtained at pH of 4.1, temperature of 70 ℃ and agitation speed of 140 rpm. The optimisation produced a polynomial quadratic model having insignificant lack-of-fit and low standard deviation, so that it was applicable and reliable in simulating the DE with only 0.80% of data were not described. Temperature affected the process highly, but the buffer pH, agitation speed and factorial interactions were considered not important. KM value for immobilised enzyme was better than the free glucoamylase, however, its reaction rate was slower than the free glucoamylase catalysis. Copyright © 2019 BCREC Group. All rights reserved

Pengolahan Limbah Cair Laundry menggunakan Ekstrak Biji Kelor sebagai Biokoagulan Hermida, Lilis; Agustian, Joni; Azizah, Zahratul
Jurnal Teknologi dan Inovasi Industri (JTII) Vol 4, No 1 (2023)
Publisher : Fakultas Teknik, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtii.v4i1.62

Hasil limbah cair laudry biasanya langsung dibuang ke badan air yang semakin lama akan menyebabkan pencemaran lingkungan karena tidak diolah terlebih dahulu. Pada penelitian ini dilakukan pengolahan limbah cair laundry dengan menggunakan biokoagulan ekstrak biji kelor. Biokoagulan ekstrak biji kelor diperoleh dengan cara mengekstraksi biji kelor menggunakan larutan NaCl 1 M. Pengolahan limbah cair laundry dilakukan secara batch dengan cara mencampurkan 80 ml/L ekstrak biji kelor ke dalam 100 ml limbah cair laundry yang telah diatur pH nya menjadi 6-10. Analisa turbidity, COD dan fosfat pada limbah cair laundry dilakukan sebelum dan sesudah pengolahan. Dari hasil penelitian diperoleh bahwa ini menunjukan bahwa efisiensi penyisihan kekeruhan, COD, dan Fosfat efektif dilakukan. Dengan kondisi pH optimum pada pH 6 yang menurunkan kekeruhan menjadi 15,5 NTU dengan persentase penyisihan hingga 97,22% dan COD hingga 65,05%. Sedangkan penurunan fosfat yang paling besar terjadi pada pH 8 dengan persentase penyisihan sebesar 95,48% hingga kadar fosfat menjadi 0,038 mg/L.

Penggunaan Ekstrak Biji Kelor sebagai Biokoagulan pada Pengolahan Limbah Cair Laundry Hermida, Lilis; Agustian, Joni; Kurniasari, Bela
Jurnal Teknologi dan Inovasi Industri (JTII) Vol 2, No 2 (2021)
Publisher : Fakultas Teknik, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtii.v2i2.35

Limbah cair laundry seringkali dibuang ke badan air tanpa diolah terlebih dahulu sehingga semakin lama dapat mencemari lingkungan. Pada penelitian ini dilakukan pengolahan limbah cair laundry dengan menggunakan biokoagulan ekstrak biji kelor. Biokoagulan ekstrak biji kelor diperoleh dengan cara mengekstraksi biji kelor menggunakan larutan NaCl 1 M. Pengolahan limbah cair laundry dilakukan secara batch dengan cara mencampurkan berbagai dosis (20-160 ml/L) ekstrak biji kelor ke dalam 100 ml limbah cair laundry . Analisa pH, turbidity, COD dan fosfat pada limbah cair laundry dilakukan sebelum dan sesudah pengolahan. Dari hasil penelitian diperoleh bahwa semakin tinggi dosis biokoagulan semakin rendah pH limbah cair laundry . Turbidity menurun dari 561 NTU hingga 56 NTU apabila dosis ditingkatkan dari 20 hingga 80 ml/L, tetapi apabila dosis ditingkatkan lagi angka turbidity menjadi naik. Semakin tinggi dosis koagulan semakin rendah angka COD. Kandungan fosfat menurun dari 1,724 mg/L ke 0,836 mg/L apabila dosis biokoagulan dinaikan dari 20 hingga 40 ml/L, tetapi kandungan fosfat meningkat apabila dosis biokoagulan ditingkatkan lebih lanjut.

Pengolahan Limbah Cair Laundry Melalui Proses Elektrokoagulasi Sistem Batch Recycle Menggunakan Elektroda Aluminium yang disusun Secara Bipolar Hermida, Lilis; Agustian, Joni; Anggraini, Devi Sagitha
Jurnal Teknologi dan Inovasi Industri (JTII) Vol 5, No 2 (2024)
Publisher : Fakultas Teknik, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtii.v5i2.101

Penelitian elektrokoagulasi limbah cair laundry dilakukan di reaktor yang beroperasi secara batch recycle, yang menggunakan 6 (enam) elektroda yang berupa alumunium dalam susunan bipolar. Pengaruh variasi parameter operasi (besarnya tegangan listrik dan kondisi pH limbah laundry) terhadap persentase penurunan kekeruhan, COD, dan Fosfat pada air limbah yang diamati. Kemudian kondisi operasi yang optimal di investigasi menggunakan rancangan percobaan fractional factorial taguchi. Berdasarkan hasil percobaan dapat disimpulkan bahwa tegangan listrik sangat mempengaruhi persentase penurunan kekeruhan, COD, dan Fosfat pada limbah cair laundry. Begitu juga dengan pengaturan pH limbah sebesar 4, 6, dan 8. Kondisi optimum diperoleh pada tegangan listrik 13,8 volt, jarak antar elektroda 0,5 cm, dan dengan pH 4, laju alir 800 l/jam dan waktu pengolahan 2 jam. Persentase penurunan kekeruhan pada kondisi optimum bisa mencapai 93,53%, persentase penurunan COD mencapai 75%, sedangkan untuk persentase penurunan fosfat mencapai 95,06%.

Salt-Assisted Mesostructured Cellular Foam (MCF) Silica Synthesis from Bagasse Bottom Ash for Enzymatic Starch Hydrolysis Agustian, Joni; Hermida, Lilis
Bulletin of Chemical Reaction Engineering & Catalysis 2025: BCREC Volume 20 Issue 3 Year 2025 (October 2025)
Publisher : Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.9767/bcrec.20379

Synthesis of MCF silica is presently conducted solely using TEOS and TMB, with the purpose of immobilizing amylolytic enzymes. Utilizing BBA and KCl to create the salt-assisted MCF silica present a viable option for converting a natural waste into an effective enzyme carrier, given its substantial silica content. The objectives were to produce the MCF silica, to employee the MCF silica as the glucoamylase carrier, and to know characteristics of the immobilized enzyme by conducting hydrolysis of starches. The carrier had surface area of 45.5 m2 g-1, pore volume of 0.12 cm3 g-1, pore size of 9.3 nm, and mesoporous silica type IV. Reduction in the carrier pore diameter and the medium to strong FTIR vibrations indicated free glucoamylase immobilization on carrier. The immobilization reached 88.5% efficiency, influenced by factors such as initial enzyme concentration, PO₄ buffer pH, and temperature, with agitation speed having a minor impact. This optimum value was obtained at the initial enzyme concentration of 9.0 mg mL-1, agitation speed of 120 rpm, buffer pH of 5.5, and temperature of 30°C. Hydrolysis of starches (tapioca, wheat, potato, corn) resulted in Dextrose Equivalent (DE) values ranging from 5.1% to 63.9%, with the immobilized glucoamylase showing better performance in potato starch hydrolysis (DE of 63.9%) and corn starch (DE of 45.6%). The use of BBA in synthesis of the salt-assisted MCF silica proved to be a viable and sustainable alternative for enzyme immobilization, with potential applications in industrial starch hydrolysis.

Glukoamilase amobil pada silika mesostructured cellular foam (mcf) magnetik untuk hidrolisis pati kentang Agustian, Joni; Hermida, Lilis; Rustamaji, Heri; Haviz, Muhammad
Prosiding Seminar Nasional Ilmu Teknik Dan Aplikasi Industri Fakultas Teknik Universitas Lampung Vol. 7 (2024): Prosiding Seminar Nasional Ilmu Teknik dan Aplikasi Industi (SINTA) 2024
Publisher : Fakultas Teknik Universitas Lampung

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Inovasi dan studi silika mesopori magnetik untuk penyangga imobilisasi enzim glukoamilase untuk hidrolisis pati kentang belum dilakukan karena penyangga magnetik hanya dikaitkan dengan bahan partikel nano baik murni maupun komposit. Proses imobilisasi enzim glukoamilase pada penyangga dengan kondisi operasi proses divariasikan untuk diketahui pengaruhnya terhadap proses dimana hasil akhir adalah tingkat imobilisasi enzim yang cukup besar yang disimpulkan dari hasil analisis kandungan protein (metode Bradford), FTIR dan SEM-EDX. Bahan partikel nano magnetik berhasil diinkorporasi pada permukaan silika MCF (9.2T-3D) dengan tingkat imobilisasi optimum enzim glukoamilase bebas adalah sekitar 89,46% yang didapatkan pada kondisi operasi optimum pH larutan buffer 5,0, suhu operasi 35°C, kecepatan pengadukan 120 rpm,dan konsentrasi enzim suplai sebesar 5,5%. Walaupun unit aktivitas enzim amylase amobil adalah lebih rendah daripada enzim amylase bebas, enzim amobil tetap dapat digunakan untuk menghidrolisis susbtrat larut dalam air. Poses hidrolisis pati kentang dengan enzim tersebut menghasilkan nilai DE optimum 26,93% pada suhu 70°C, kecepatan pengadukan 170 rpm, pH buffer asetat 0,1 M 4,6, dan konsentrasi enzim 1500 U/mg.

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