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All Journal Biosaintifika: Journal of Biology & Biology Education
Herry Utomo
LSU AgCenter H.Rouse Caffey Rice Research Station Crowley LA (70578) USA
Agriculture, Biological Sciences & Forestry

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Orchid Mycorrhizal Fungi Combination to Overcome Water Stress in Dendrobium Spectabile Orchids R. Soelistijono; Prisma Aditya Rianto; Achmad Fatchul Aziez; Cecep Hidayat; Herry Utomo
Biosaintifika: Journal of Biology & Biology Education Vol. 18 No. 2 (2026): August 2026
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v18i2.42840

Abstract

One strategy to mitigate drought stress is using orchid mycorrhizal fungi (OMF) of the Rhizoctonia type. This study aims to determine the use of a combination of Rhizoctonia mycorrhiza isolated from the roots of two Dendrobium orchid species and watering intervals on Dendrobium spectabile seedlings. The research method used was a randomized block design (RBD) with 2 factors and 5 replications: the first factor was inoculation of OMF isolated from the roots of Dendrobium fimbriatum (D1), Dendrobium phalaenopsis (D2), and a combination of OMF D1 + D2 (D3). The second factor included watering intervals every 2-days (W1), 4 days (W2), and every 6-days (W3). The results showed that OMF inoculation factors on D. spectabile gave significantly different results on plant height, leaf length, root length, plant fresh weight, and plant dry weight, with the best results using the OMF combination (D3). The watering interval factor gave significantly different results on plant height and number of roots, with the best results on watering every 4-days (W2). The highest chloroplast content was found in D3W3 (0.89), while proline accumulation reached its peak at D2W3 (0.135). Microscopic observations confirmed the formation of pelotons in the root cortex. Overall, the combined OMF inoculum applied with a 4-day watering interval (D3W2) is a promising approach to improve drought tolerance in D. spectabile seedlings. This research supports SDG 15 (Life on Land Ecosystems), as a solution to overcome water stress, the main cause of desertification and land degradation that affects the growth of orchid species in nature.
Utilization of Rhizoctonia Mycorrhizae for Orchid Late Blight Control in Sustainable Agricultural R. Soelistijono; Daryanti Daryanti; Dian Rakhmawati; Prisma Aditya Rianto; Herry Utomo
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 1 (2024): April 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i1.3498

Abstract

Vanda tricolor is currently a protected species because is the occurrence of orchid late blight caused by the Fusarium sp. pathogenic fungus. The objective of this study was to investigate the effectiveness of Rhizoctonia mycorrhizae in inducing resistance against Fusarium sp., the causative agent of late blight, in V. tricolor. The research employed CRBD with three treatments and six replications. Rhizoctonia mycorrhizae were isolated and identified following the procedure outlined by Bayman. Pre-inoculation of Rhizoctonia mycorrhizae was conducted on PDA media, and mycorrhizae were subsequently inoculated onto the roots of V. tricolor seedlings derived from tissue culture. The presence of peloton structures on the roots was determined using the Nakano methods. The Saravanan method was employed to measure peroxidase activity. The results demonstrated that V. tricolor induced by Rhizoctonia mycorrhizae exhibited resistance against Fusarium sp. and displayed the formation of peroxidase enzymes, peloton structures, and lignification. In contrast, the non-induced orchids suffered significant damage to the epidermal tissue of the leaves, did not exhibit an increase in peroxidase enzymes, and failed to form peloton or lignified structures. The novelty of this research is the use of Rhizoctonia mycorrhizae to control orchid late blight diseases at Mount Merapi.
Co-Authors Achmad Fatchul Aziez Cecep Hidayat Daryanti Dian Rakhmawati Prisma Aditya Rianto Prisma Aditya Rianto R. Soelistijono R. Soelistijono