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All Journal Warta IHP (Warta Industri Hasil Pertanian)
Sari, Rahma
Balai Besar Industri Agro
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering

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(Gynura pseudochina (Lour.)DC).(Analysis for Pyrolizidine contents in Dried Herbal Tea of Daun Dewa (Gynura pseudochina (Lour.) DC) Sari, Rahma; Hartanto, Eddy; Farida Hutajulu, Tiurlan
Warta Industri Hasil Pertanian Vol 26, No 02 (2009)
Publisher : Balai Besar Industri Agro

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Abstract

Dewa leaf (Gynura pseudhochina (Lour) DC) often used as herbal tea to its benefit in reducing blood glucose content. One of the active compound in dewa is pyrrolizidine alkaloid. which is active against tumor. The aim of this research was to identify the active compound of pyrrolizidine in leaves of dewa green tea, black tea and dry powder which are resulted from the experiment. The analysis of pyrolizidine was done by quantitative and qualitative methods. The separation method used for the pyrrolizidine compounds was thin layer chromatograpy using methanol:water(85 : 15) as developing solvent. The determination of pyrrolizidine content was done by TLC scanner. The result showed that the RF value of dewa green tea is 0.34 relatively equal to the pyrrolizidine standard (0.360. While the Rf value of pirolyzidine compound in leaves of dewa black tea and dry powder was 0.42 and 0.41 significantly different from pyrrolizidine standard. The pyrrolizidine compound in leaves of dewa black tea and dry powder could not be detected quantitavely by TLC scanner because its concentration was too small, where as in leaf of dewa green tea the pyrrolizidine compound was detected 0.005%. So, it could be concluded that the contents of pyrrolizidine compound in leaf of dewa green tea was higher than pyrrolizidine content in black tea and dry powder of dewa leaf.
(Indentification of Phenol and Delphinidine in the Telang’s flower (Clitoria ternatea L.) and its effectivity to staphylococcus aureus as Eyes Bacterial Deseases) Farida Hutajulu, Tiurlan; Sari, Rahma; -, Djumarman
Warta Industri Hasil Pertanian Vol 25, No 02 (2008)
Publisher : Balai Besar Industri Agro

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Abstract

Triglicoside, phenol and delphinidine are compounds, which is found in the telangs flower(Clitoria ternateaL.) Telangs flower usually used by the local people as traditional medicinal plant for eyes conjungtivitis desease caused by Staphylococcus aureus bacteria. The aim of the study is to know the effectivity of telangs flower on conjungtivitis. The preparate of the extracts was prepared by maseration of 1,3 and 5%of telangs flower using 100ml aquabidest for 24 hours. The extract was then freeze dried at-15C. The extract obtained was analised by Thin layer chromatography (TLC) using two types of eluent  (butanol: asetic acid: water = 4: 4: 2) and eluent (chioroform: methanol =9:1).The spots were obserbed visually under ultraviolet ray at 254 nm and the spots were scrape of, then disolve in 10ml of aquabidest. The extract then tested by Staphylococcus aureus bacteria using the paper diffusion method. The results show that the extract of telangs flower 1% contains 0.014% of delphinide, 0.020% phenol, and 3% extract were 0.016% of delphinidine, 0.022% phenol, and 5% extract were 0.017% of delphinedine, and 0.026% of phenol. The phenol compound 0.026%showed an inhibition areas of 0.87mm as antibacterial while delphinedine is just a blue pigment of telangs flower.
Co-Authors -, Djumarman Farida Hutajulu, Tiurlan Hartanto, Eddy