Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal Sumatera Medical Journal
Muhammad, Noorzaid
Unknown Affiliation
Immunology & microbiology Medicine & Pharmacology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Diets That Have Potential to Stimulate the Hypothalamic-Pituitary-Adrenal (HPA) Axis in Sprague Dawley Rats Khairil Azwan; Mona, Resni; Firdous, Jannathul; Rosie David, Pamela; Keumala Sari, Dina; Muhammad, Noorzaid
Sumatera Medical Journal Vol. 6 No. 2 (2023): Sumatera Medical Journal (SUMEJ)
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/sumej.v6i2.11650

Abstract

Background: The pituitary gland is the master gland regulating body hormones. From the anterior pituitary’s corticotrophs, adrenocorticotrophic hormone (ACTH) is secreted and influences corticosterone production in rodents via the hypothalamic–pituitary–adrenal (HPA) axis. ACTH and corticosterone are commonly used as hormonal stress markers. Disturbances in ACTH and corticosterone are seen in diseases like Addison’s disease, Cushing’s syndrome, and metabolic syndrome. Objective: To observe which diet induces the most and least stress on Sprague Dawley rats’ physiology. Methods: Thirty-five Sprague Dawley rats were divided into five groups (n=7) and given different diets (control, high-fat, high-protein, high-sugar, and high-starch) with tap water ad libitum. After eight weeks, blood samples were collected and serum separated. ACTH and corticosterone were extracted, purified, identified, and quantified using High-Performance Liquid Chromatography (HPLC) with photodiode array (PDA) analysis. Results: High-sugar diet led to the highest ACTH blood level. Both high-fat and high-sugar diets showed the highest corticosterone peaks. Conclusion: Consumption of high-fat and high-sugar diets for eight weeks is suggested to induce physiological and metabolic stress in Sprague Dawley rats, as evidenced by HPA axis activation.
Subacute Toxicity of Yellow Flower Extract (Cananga odorata) on the Histopathology of White Rat Heart (Rattus norvegicus) Mani, Suresh; Akbari Ganie, Ratna; Keumala Sari, Dina; Meutia Sari, Liza; Rachmawati, Meike; Muhammad, Noorzaid
Sumatera Medical Journal Vol. 6 No. 2 (2023): Sumatera Medical Journal (SUMEJ)
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/sumej.v6i2.11931

Abstract

Background: The inflammatory process is influenced by chemical mediators such as Nitric Oxide (NO), cytokines, and arachidonic acid metabolites. The therapeutic effect of herbal plants comes from phytochemicals with strong antioxidant activity. Objective: To examine the phytochemical content and anti-inflammatory effects of Cananga odorata. Methods: This was a two-stage study: in vitro and in vivo. Anti-inflammatory activity is related not only to antioxidant properties but also to suppression of nitric oxide and pro-inflammatory cytokines. This effect is being explored using various herbal plants. One such plant is Cananga odorata (ylang flower), a tropical plant with fragrant petals that grows easily. Results: The total phenol content was 1.06% w/w. Antioxidant activity measured using DPPH method showed an IC50 of 808.86 ppm, indicating weak antioxidant activity. In rats, the extract reduced blood glucose levels but had no effect on lipid profiles. Toxicity testing showed no toxic effects. Histological analysis showed no differences between cardiovascular disease model mice and standard-fed mice. Conclusion: The ethanol extract of Cananga odorata did not show toxicity in the heart histopathology of white rats.
Co-Authors Akbari Ganie, Ratna Firdous, Jannathul Keumala Sari, Dina Khairil Azwan Mani, Suresh Meike Rachmawati Meutia Sari, Liza Mona, Resni Rosie David, Pamela