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Denny Juraijin
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Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Environmental Science Health Professions Immunology & microbiology Nursing Public Health

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PENGARUH LAMANYA PENUNDAAN TERHADAP PEMERIKSAAN ELEKTROLIT KALIUM Denny Juraijin
Masker Medika Vol 11 No 2 (2023): Masker Medika
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/maskermedika.v11i2.573

Abstract

Background : Electrolytes have a crucial role in the human body which can affect metabolism, so it needs to be maintained in balance. Imbalances of electrolytes in the body need to be anticipated by measuring or examining these electrolyte levels so that they can detect disease, establish a diagnosis, determine prognosis, become a guide in patient management, and monitor treatment. Errors in examination can have serious consequences that can lead to errors in treatment because they are based on inaccurate results. Sample handling is one of the causes of the inaccuracy of the results of the examination. It is not permissible to leave blood samples for checking electrolyte levels for too long. Therefore, this research is needed regarding the effect of delaying the examination of blood electrolytes, especially potassium, on the examination results. Research Objectives : To determine the effect of the long delay of the examination on Potassium electrolyte levels in samples of immediate examination, 12-hour delay, and 24-hour delay. Research Methods : This study used an experimental method using 30 blood samples from patients at AR Bunda Prabumulih hospital in January. Results : The average value of electrolyte levels on immediate examination was 4.11. Meanwhile, on examination with a delay of 12 hours, the average electrolyte level of Potassium was 4.10, and for the examination results from a delay of 24 hours, the electrolyte level of Potassium was 4.12. To prove whether there is an effect of delaying the inspection of Potassium on the examination results, a statistical test is carried out with the SPSS application. Conclusion : Based on the results of this study, it was stated that there was no effect of the length of delay on examining potassium electrolytes and blood samples with a delay of up to 24 hours could still be used to check blood electrolyte levels.
Perbedaan Derajat Aglutinasi Pemeriksaan Golongan Darah Metode Tabung Berdasarkan Konsentrasi Suspensi Sel 5% Segera Periksan Dengan Lama Penyimpanan 5 Hari Dewi Hartati; Hanny Rizky Ananda; Denny Juraijin
jurnal kesehatan terapan sains dan teknologi Vol 2 No 1 (2024): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v2i1.32

Abstract

Background: ABO b;ood group examination is an examination carried out to determinate the type of blood group in humans that detects the presence of antigens on the surface of the red blood cell membrane by means of the Tube Method various consentrations of erythrocyte cell suspension,namely 5%, 10% and 15%. Research purpose: To determinate the effect of various consentrations of erythrocyte cell suspension with ready-to-use 0,9% NaCl and 0,9% table salt solvent on the degree of agglutination in blood group examination Tube method. Research method: This research is a pure experimental study with a total sample 41 people, this study was conducted on January 16-20, 2023 at the Muhammadiyah Palembang IKesT Clinical Pathology Laboratory. Results: The results showed that the blood group examination was done by the tube method with a concentration of erythrocyte cell suspension, namely 5%, 10% and 15% with 0,9% NaCl solvent ready-to-use and 0,9% NaCl, table salt solvent resulting in a degree of agglutination of +4. The Kruskal-wallis test result in the Cell Grouping method obtained 0,000 and in the Serum Grouping method obtained 0,051. Conclusion: The conclusion in this study is that there is no effect of various consentrations of erythrocyte cell suspension with ready-to-use 0,9% NaCl and 0,9% NaCl Solvent Kitchen Salt on the degree of agglutination in blood group examination Tube Method so that 0,9% NaCl solvent from table salt can be used as an alternative to ready-to-use 0,9% NaCl reagent in blood group tests by tube method. Keywords: Blood type, Aglutination
PERBEDAAN HASIL BAKTERI TAHAN ASAM METODE ZIEHL NEELLSEN DAN TES CEPAT MOLEKULER (TCM) DENGAN LAMA PENYIMPANAN 48 JAM Aristoteles; Yuliana Maya Sari; Denny Juraijin
jurnal kesehatan terapan sains dan teknologi Vol 2 No 1 (2024): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v2i1.33

Abstract

Background: Tuberculosis (TBC) is an infectious disease caused by Mycobacterium tuberculosis, a dorect human-to-human infectious disease not caused by witchcraft or curses TB germ most often attack the lungs but can also attack other organs such as the lymph nodes, bones, brain, skin and others. The diagnosis of pulmonary TB is established based on the clinical picture, physical examination, radiological picture, laboratory examination and tuberculin test. The diagnosis of pulmonary tuberculosis currently used routinely in labpratories including hospitals and health centers is a bacteriological diagnosis using acid-fast bacillus microscopic techniques (BTA) and molecular rapid test (TCM). TCM examination is a molecular detection methode based on nested real-time PCR. The use of TCM is a priority for TB examination because it has several advantages, including: High sensitivity, fast and results can be known in approximately 2 hours. Objectives: To determine differences in the results of acid-fast bacteria Ziehl Neelsen method and the molecular rapid test (TCM) with a storage time of 48 hours. Method: This type of research is pure experiment. The research was conducted in the Celikah Health Center laboratory in February 2023 with a total sample of 5 smear samples (+1). Result: The results showed that there was no difference in the tesults of acid-fast bacteria between the ziehl neelsen method and the molecular rapid test (TCM) for 48 hours of storage. The wilcoxon test results obtained a value of p = 0,157. Conclusion: The molecular rapid test method has better sensitivity and specificity in the diagnosis of tuberculosis thab microscopic examination using ziehl neelsen staining.
Co-Authors Aristoteles Dewi Hartati Hanny Rizky Ananda Yuliana Maya Sari