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Delima Engga Maretha
Department of Biology, Faculty of Science and Technology, UIN Raden Fatah, Jl. K.H Zainal Abidin Fikry, Km 3,5 Palembang, Indonesia, 30126
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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Phenolic Content and Antioxidant Activity Water and Ethanol Extracts of Sungkai Leaves (Peronema canescens Jack) Delima Engga Maretha; Dwi Fitri Yani; Lili Silvana; Mashuri Masri
Jurnal Biodjati Vol 8, No 2 (2023): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i2.26777

Abstract

Sungkai (Peronema canescens Jack) is a plant that has phenolic content as an antioxidant that can enhance the immune system (immunomodulator). This study aimed to determine the phenolic content and antioxidant activity of Sungkai leaves (Peronema canescens Jack). The method  of antioxidant with invitro using of the DPPH where the ability of a compound to inhibit DPPH radicals is calculated from the absorbance value using UV-Vis spectroscopy. And Determination of total phenolic content was carried out using UV VIS spectroscopy at a wavelength of 745 nm with the help of Folin Ciocalteu reagentand a nd gallic acid standard curve . The test results showed that the water and ethanol extracts with a concentration of 0.1% had a phenolic content of 0.002% and 0.007%, their antioxidant activity (IC50) with a concentration of 0.02; 0.04; 0.06; 0.08; and 0.1% in the water and ethanol extracts of Sungkai leaves have values of 0.025% and 0.03% respectively. Therefore the best antioxidant activity (IC50) was extracts ethanol of sungkai leaves with phenolic content  of 0.007% and IC50  of 0.03%.
Phenolic Content and Antioxidant Activity Water and Ethanol Extracts of Sungkai Leaves (Peronema canescens Jack) Delima Engga Maretha; Dwi Fitri Yani; Lili Silvana; Mashuri Masri
Jurnal Biodjati Vol 8 No 2 (2023): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i2.26777

Abstract

Sungkai (Peronema canescens Jack) is a plant that has phenolic content as an antioxidant that can enhance the immune system (immunomodulator). This study aimed to determine the phenolic content and antioxidant activity of Sungkai leaves (Peronema canescens Jack). The method  of antioxidant with invitro using of the DPPH where the ability of a compound to inhibit DPPH radicals is calculated from the absorbance value using UV-Vis spectroscopy. And Determination of total phenolic content was carried out using UV VIS spectroscopy at a wavelength of 745 nm with the help of Folin Ciocalteu reagentand a nd gallic acid standard curve . The test results showed that the water and ethanol extracts with a concentration of 0.1% had a phenolic content of 0.002% and 0.007%, their antioxidant activity (IC50) with a concentration of 0.02; 0.04; 0.06; 0.08; and 0.1% in the water and ethanol extracts of Sungkai leaves have values of 0.025% and 0.03% respectively. Therefore the best antioxidant activity (IC50) was extracts ethanol of sungkai leaves with phenolic content  of 0.007% and IC50  of 0.03%.
Co-Authors Dwi Fitri Yani Lili Silvana Mashuri Masri