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Sabarina Elfrida Manik
Universitas Binawan
Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology

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PENGARUH VARIASI WAKTU SENTRIFUGASI NILAI HEMATOKRIT MENGGUNAKAN METODE MIKROHEMATOKRIT DI UNIVERSITAS BINAWAN Nurcholis Majid; Sabarina Elfrida Manik; Intan Kurniawati Pramitaningrum
Jurnal SainHealth Vol. 7 No. 2 (2023): September 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i2.14334.51-57

Abstract

Hematocrit is the total volume of red blood cells in 100 ml of blood expressed as a percentage of blood volume. Hematocrit examination is needed to help diagnose diseases such as anemia and dengue hemorrhagic fever (DHF) to determine the total volume of red blood cells or erythrocytes. The normal value for hematocrit examination is 40-54% for males and 37-47% for females. The purpose of this study was to determine the effect of variations in centrifugation time on hematocrit values using the microhematocrit method. This type of research is quantitative using experimental method with cross sectional design. The method used for hematocrit examination is the microhematocrit method with variations in centrifugation time of 2 minutes, 3 minutes, 4 minutes, and 5 minutes as a control with a speed of 12,000 rpm. The respondents of this study were 35 respondents and the samples of this study were 35 venous blood samples taken from Binawan University students, Medical Laboratory Technology Study Program batch 2021 and 2022. The average result of the 2-minute centrifugation hematocrit value examination is 37.87%, 3-minute centrifugation is 37.57%, 4-minute centrifugation is 37.30%, and 5-minute centrifugation is 37.25%. The Wilcoxon test results show the Sig. (2-tailed) of 2-minute centrifugation compared with 5 minutes is 0.00 (<0.05), 3-minute centrifugation compared with 5 minutes is 0.001 (<0.05), 4-minute centrifugation compared with 5 minutes is 0.560 (>0.05). The results of this study showed that hematocrit values with centrifugation times of 2 minutes and 3 minutes compared to 5 minutes had significant differences in hematocrit values.
ANALISIS PENERAPAN PRA-ANALITIK DAN ANALITIK KUALITAS SEDIAAN BTA METODE ZIEHL-NEELSEN DI PUSKESMAS KECAMATAN CIRACAS JAKARTA TIMUR Cut Nur Masita; Dian Rachma Wijayanti; Sabarina Elfrida Manik
Jurnal SainHealth Vol. 8 No. 1 (2024): Maret 2024
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v8i1.14358.1-5

Abstract

The microscopic examination of BTA with sputum samples aims to detect the onset and monitoring of treatment in tuberculosis. The purpose of this study is to find out the Analysis of the application of six preanalytic and analytical indicators to the quality of acid-resistant basil preparation using the Ziehl-Neelsen method at the East Jakarta Ciracas Subdistrict Health Center, which was conducted in April-June 2023. The type and design of the study are analytical cross-sectional, data analyzed using the Chi-Square test. BTA prepared quality results by performing six indicators, proportionally greater good prepared quality than poor prepared quality with 90% specimen quality percentage, 95% preparation quality, 85% preparation cleanliness, 90% preparation size, 92.5%, and 92.5% preparation accuracy. An analysis of the application of six pre-analytic and analytic indicators to the quality of the acid-resistant basil supply using the Ziehl-Neelsen method at the East Jakarta Ciracas Subdistrict Health Center which includes specimen quality, preparation cleanliness, preparation thickness, and preparation accuracy showed a meaningful relationship. The coloring of the preparedness and the size of the preparedness indicates no meaningful relationship.
PERBANDINGAN KADAR TROMBOSIT PADA PEMBENDUNGAN VENA SELAMA 1 MENIT DAN 2 MENIT: COMPARISON OF PLATTLE LEVELS IN VEIN DAMMING FOR 1 MINUTE AND 2 MINUTES ari ardiyanto sardi; Sabarina Elfrida Manik; Dian Rachma Wijayanti
Jurnal SainHealth Vol. 8 No. 1 (2024): Maret 2024
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v8i1.14360.11-14

Abstract

Phlebotomy is the process of drawing blood from the circulatory system through a puncture to obtain a sample. Venous blood collection is done using a tourniquet as a damming device that serves to see the vein under the skin tissue. In adults, one of the veins used is the cubital fossa and in infants using the superficial jugular vein or in the superior sagittal sinus. Platelets are disk-shaped cells with a diameter of 2-5 mm. Under normal circumstances, the number of platelets is about 150,000-450,000 / ml of blood and its life span is about 1- 2 weeks or about 8 days. Platelets are made of phospholipid substances that are important for clotting, maintaining the integrity of blood vessels and repairing damaged small blood vessels. The purpose of this study was to determine the comparison of platelet levels in vein damming at 1 minute and 2 minutes. This study was conducted in the MM2100 industrial area, samples obtained from employees who did Medical Check Up and obtained as many as 30 respondents and 60 samples. The results showed that employees in the MM2100 industrial area with average platelet levels of 274,133/µl of blood at 1 minute and average platelet levels of 278,266/µl of blood at 2 minutes. This study uses the Paired Sample T-Test method, the results obtained with a sig value of 0.229 which means there is no difference in the damming time of 1 minute and 2 minutes on platelet levels.
Co-Authors ari ardiyanto sardi Cut Nur Masita Dian Rachma Wijayanti Intan Kurniawati Pramitaningrum Nurcholis Majid