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All Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST ALCHEMY Jurnal Penelitian Kimia BIOEDUSCIENCE Meditory : The Journal of Medical Laboratory Jurnal SainHealth JMM (Jurnal Masyarakat Mandiri) Biota GERVASI: Jurnal Pengabdian kepada Masyarakat Medical Technology and Public Health Journal Biopendix: Jurnal Biologi, Pendidikan dan Terapan Borneo Journal of Medical Laboratory Technology BioEksakta : Jurnal Ilmiah Biologi Unsoed Binawan Student Journal Jurnal Multidisiplin Madani (MUDIMA) Jurnal Pengabdian Masyarakat Bhinneka JMLS: Journal of Medical Laboratory and Science

Dian Rachma Wijayanti

Universitas Binawan

Author-ID : 1806462

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Isolation and Characterization Of Arbuscular Mychorhiza Fungi from Gaharu Wood (Aquilaria spp.) Rhizosphere Dian Rachma Wijayanti; Maman Turjaman
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3090

In the last 30 years there have been increasing demand of agarwood. Unlimited hunting of agarwood causing it become threatened species. Aquilaria spp. is the main genus producing agarwood that considered as threathened species. Conserving Aquilaria spp. as germ plasm needs is essential against destruction. Arbuscular mycorrhizal fungi (AMF) have been used as inoculant to increase the growth of Aquilaria spp., but information about the existence of AMF in Aquilaria spp. rhizosphere has not been reported. This research was carried out with objective to identify AMF from Aquilaria spp. rhizosphere. Soil and root samples from rhizosphere of Aquilaria spp. taken from four location that is: Carita (A. malaccensis), Ciapus (A. filaria), Cifor (A. crasna & A. malaccensis), and Bantar Kemang (A. beccariana, A. crasna, & A. malaccensis). Roots staining was conducted with modified Phillips and Hayman procedure (1970). Soils sample was run dry wed and placed in pot culture with Pueraria javanica as the host plant. Spore was separated from pot culture using wet sieving and decanting method continued with centrifugation. The result showed the occurrences of AMF in Aquilaria spp. roots i.e. external hypha, internal hypha, coiled hypha, arbuscules, and vesicles. All AMF colony structure found in Aquilaria spp. roots show asosiation bettwen the plant and AMF. Four species of AMF were found i.e. Glomus sp.1, Glomus sp.2, Gigaspora albida, and Dentiscutata sp. Key Words: Isolation, Arbuscule Mycorrhizae Fungi, Rhizosfer, Agarwood

Extraction and Identification Potent Antibacterial Bioactive Compound of Streptomyces sp. MB 106 from Euphorbia sp. Rhizosphere Dian Rachma Wijayanti; Almatin Puspa Dewi
BIOEDUSCIENCE Vol 6 No 1 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Background: Actinomycetes are groups of bacteria that play an important role in pharmacy and medicine in their ability to produce secondary metabolites in the form of bioactive compounds with various chemical structures and biological activities. The genus Streptomyces produces more than 70% of all described actinomycetes natural products. In particular, antibiotics represent the largest group. Through mutations, bacteria may survive chemotherapy. Thus its infection is hard to eradicate. Therefore the search for new potent bioactive compounds is important Methods: Isolate preparation, Production, and extraction bioactive compound, Crude extract antibacterial test, Identification of bioactive compound Results: There are antibacterial activities per 100 µl crude extract. Inhibition zone range from 11.5 to 13 mm. Various bioactive compounds detected in n-Butanol extract, There are nine bioactive compounds detected from Streptomyces sp. MB 106 crude extract. The highest was Naphthalene (43.89 %). The lowest was 3-Imino-3h,5h- [1,2,4] Thiadiazolo [3,4-1] Isoindole (1.70 %). Six compounds were reported to have antimicrobial activities butane, 1,1-dibutoxy, naphthalene, nonadecane, docosane, heneicosane, and eicosane Conclusions: Crude extract showed an average of 12 mm inhibition zone against Escherichia coli ATCC 8739. There are nine bioactive compounds from Streptomyces sp. MB 106. Six of them are promising drugs candidate. Further studies on these compounds are essential for future drugs candidate.

KORELASI KADAR AST DAN ALT CALON TENAGA KERJA INDONESIA PENDERITA HEPATITIS B DENGAN PERILAKU HIDUP BERSIH DAN SEHAT DI AN-NUR MEDICAL CENTER TAHUN 2020 Indah Andriyanti; Aturut Yansen; Dian Rachma Wijayanti
Binawan Student Journal Vol. 3 No. 3 (2021)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Binawan (LPPM Universitas Binawan)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54771/bsj.v3i3.411

Hepatitis B merupakan penyakit hati yang disebabkan oleh infeksi virus hepatitis B (HBV). Infeksi dari HBV ini dapat menyebabkan peradangan hati akut ataupun kronis yang dapat berlanjut menjadi sirosis hati atau kanker hati. Gambaran klinis, evaluasi tes fungsi hati merupakan kriteria yang digunakan dalam mendiagnosis penderita hepatitis B. Peningkatan kadar spartate Aminotransferase (AST) dan Alanin Aminotransferase (ALT) merupakan salah satu kriteria penting dalam mendiagnosis pasien dengan hepatitis B. Indonesia adalah negara endemik hepatitis B, salah satu faktor yang menyebabkan kasus hepatitis B meningkat di negara Indonesia dikarenakan kurangnya kesadaran tentang PHBS, kurangnya pengetahuan, dan faktor kelelahan. Penelitian ini bertujuan untuk mengetahui hubungan kadar AST dan ALT pada penderita hepatitis B calon TKI. Datadiambil dari data primer pasien hepatitis B yang melakukan pemeriksaan kadar AST dan ALT periode Januari – April 2020 di klinik An-Nur Medikal Center. Jenis penelitian ini adalah deskriptif kuantitatif dengan pendekatan cross sectional dengan jumlah sampel 38. Data analisis menjelaskan bahwa ada hubungan antara wilayah endemik dengan peningkatan kadar AST dan ALT faktor kelelahan, dan HBsAg dengan kadar AST dan ALT pada calon TKI. Sehingga dapat disimpulkan bahwa wilayah endemik, faktor kelelahan, dan HbsAg dapat mempengaruhi peningkatan kadar AST dan ALT.

CORRELATION OF INTERLEUKIN-6 (IL-6) LEVEL AND NUMBER OF LYMPHOCYTES IN COVID-19 POSITIVE PATIENTS AT PERSAHABATAN HOSPITAL Mimah Mulhimah; Dian Rachma Wijayanti; Frida Octavia Purnomo
TEKNOLOGI MEDIS DAN JURNAL KESEHATAN UMUM Vol 6 No 1 (2022): Medical Technology and Public Health Journal March 2022
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mtphj.v6i1.2768

Covid-19 caused by the SARS-CoV-2 virus and is transmitted through droplets. In Covid-19 patients who are critical or have severe symptoms, hypercytokines are often found, which is characterized by an increase in the cytokine Interleukin-6 (IL-6). High levels of IL-6 cytokines can disrupt the immune system, including leukocytes, especially lymphocytes, which are the main system of the immune response. The state of continued inflammation and continuously stimulated lymphocytes will cause the lymphocytes to work too hard. This study aims to determine the relationship of Interleukin-6 (IL-6) with lymphocytes in Covid-19 positive patients. This study is a quantitative research with correlation analysis method using data collection technique by purposive sampling. Furthermore, to analyze, the authors used the Kolmogorov-Smirnov normality test and the Spearman correlation test. The results of the analysis showed a fairly significant correlation between levels of Interleukin-6 (IL-6) and the number of lymphocytes in Covid-19 positive patients.

Analisis Sensitivitas Dan Spesifisitas COVID-19 Ag Rapid Test Terhadap PCR Test Wisnu Murti; Dian Rachma Wijayanti; Wulan Fitriani Safari
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i2.12148

SARS-Cov-2 is the newest coronavirus caused Covid-19 which can infect human and pandemic, COVID-19 parameter testing can be done with serological tests used to detect antibody or antigen and also molecular test using PCR method. This research is aimed to compare the sensitivity and specificity between COVID-19 Ag rapid test and PCR test. This research is an analytical descriptive study with 421 samples were obtained from documentation study with probability systematic random sampling and by using secondary data taken from Laboratory Information System (LIS) at Siloam Hospitals Lippo Village Tangerang and the type of observational analytical research with cross sectional design. The analysis results show the sensitivity rate was 96.64%, specificity was 93.75%, PPV was 89.44%, NPV was 98.08%, LR (+) was 15.46, and LR (-) was 0.04. The conclusion of the research result, COVID-19 Ag rapid test is valid to be used as a diagnostic test because it has high rate of sensitivity and specificity according to WHO regulations.

CEMARAN BAKTERI SALMONELLA SP DAN HIGIENIS SANITASI PADA DAGING AYAM DIRUMAH AYAM POTONG UD BERKAH PUTRI MANDIRI Yazit Albustomi; Dian Rachma Wijayanti; Septiani Septi
Journal of Medical Laboratory and Science Vol 2 No 1 (2022): JMLS: Journal of Medical Laboratory and Science
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Palembang

Kesehatan adalah hal mutlak yang harus dijaga yaitu dengan memenuhi kebutuhan pokok manusia salah satunya adalah makanan. Dengan makanan nutrisi yang tercukupi upaya untuk menjaga tubuh tetap sehat dan terjaga, kemudian makanan yang mudah terjangkau dan relative murah dengan protein , lemak dan mineral yang cukup didapatkan adalah daging ayam. Namun pada aspek mikrobiologi seuatu pangan aman dikonsumsi jika produk tersebut aman dari mikroba pathogen salah satunya bakteri Salmonella Sp. Tujuan penelitian ini untuk mengetahui status higienis sanitasi dan cemaran bakteri Salmonella Sp. Metode penelitian yang digunakan untuk penelitian ini adalah observasional, menggunakan sampel sesuai dengan kriteria peneliti sebanyak 7 sampel serta mewawancarai pengelola RPA terkait dengan pertanyaan higienis dan sanitasi. Hasil penelitian yang menggunakan uji bakteri Salmonella sp (SNI 2897 : 2008) tidak didapatkan cemaran bakteri Salmonella Sp pada media serta pada hasil pengolahan data wawancara dari pengelola Rumah Pemotongan Ayam didapatkan hasil pada wawancara tentang higienis karyawan dan peralatan sebesar 80 %, Persyaratan peralatan 87,5%, persyaratan sarana 80% dan Persyaratan pengolahan daging 60 % keseluruhan hasil dari wawancara RPA hampir memenuhi syarat dari SNI 01-6160-1999. Kesimpulan dari tugas akhir ini adalah Sebanyak 7 sampel dinyatakan tidak tercemar bakteri Salmonella Sp, kemudian pada beberapa sampel ditemukan dugaan bakteri lain yang tumbuh disalah satu media serta kuisioner yang didapat berdasarkan wawancara hampir memenuhi persyaratan.

A URINE GLUCOSE LEVELS AND URINE SPECIFIC GRAVITY IN TYPE 2 DIABETES MELLITUS PATIENTS IN BUDHI ASIH HOSPITAL Arigayota Darwin Mahara Kala; Dian Rachma Wijayanti; Mohamad Syafaat
Journal of Medical Laboratory and Science Vol 2 No 2 (2022): JMLS: Journal of Medical Laboratory and Science
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Palembang

Background: Diabetes mellitus is caused by the pancreas not being able to produce the insulin hormone that regulates glucose in the body, resulting in hyperglycemia which is associated with long-term damage, dysfunction, failure of various organs (eyes, kidneys, nerves, heart, and blood vessels). Diabetes is included as a non-communicable disease but has a priority for follow-up because the number of cases and its prevalence has continued to increase over the last few decades. Examination of diabetes mellitus can be done with blood samples and urine samples. Examination of diabetes mellitus with urine samples was carried out using the dipstick method to determine the parameters of urine glucose and urine specific gravity. Objective: To determine the correlation of urine glucose and urine specific gravity in patients with type 2 diabetes mellitus. Methods: Correlative analytic study with a cross-sectional approach. This study was conducted in January-September 2021 with samples of type 2 diabetes mellitus patients who examined urine glucose and urine specific gravity with the dipstick method. Results: The results of the correlation test of urine glucose and urine specific gravity in patients with type 2 diabetes mellitus are 0.065 (> 0.05) which means that there is no correlation between urine glucose and urine specific gravity in patients with type 2 diabetes mellitus. The results of the correlation coefficient of urine glucose and specific gravity urine in patients with type 2 diabetes mellitus is 0.189 which is included in the range of weak correlation coefficients. Conclusion: Based on these results, it can be concluded that there is no significant correlation between urine glucose and urine specific gravity in patients with type 2 diabetes mellitus.

Overview of Third Trimester Pregnant Women's Urine Leukocyte Sediment and Protein Levels at the Laboratory of Anggrek Mas Hospital, West Jakarta Halimah Nurul Istiqomah; Septiani; Muhammad Rizki Kurniawan; Dian Rachma Wijayanti
Jurnal Multidisiplin Madani Vol. 3 No. 4 (2023): April 2023
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/mudima.v3i4.2818

Pregnant women with the third trimester of pregnancy (7-9 months or 29-40 weeks), most of them give birth normally, but 15% of cases are life-threatening complications, namely preeclampsia. Preeclampsia is characterized by increased proteinuria. Women during pregnancy are more at risk of suffering from urinary tract infections because of the physiological changes that occur in their bodies. As many as 20% of cases of urinary tract infection occur in pregnant women. Examination of urine protein and urine leukocyte sediment in pregnant women is very important as a support for the risk of preeclampsia and urinary tract infections. This study used descriptive analysis with a cross-sectional design, namely one-time observation. Data used by third trimester pregnant women. The research was conducted in January-July 2022 at the Anggrek Mas Hospital Clinical Laboratory. There are 20 research subjects. With the highest protein levels at high risk of preeclampsia were (+3) 1 pregnant woman and (+4) 3 pregnant women. Then the leukocytes of the urine sediment are above normal values≥5/Landang Besar, namely there are 12 pregnant women each. In the majority of third trimester pregnant women at Anggrek Mas Hospital, West Jakarta, all pregnant women who were observed, the results of urine protein levels were found to be abnormal and urine sediment leukocytes were found to be above normal values

EDUKASI KUMAN GIGI DAN MULUT SERTA CARA MENYIKAT GIGI DENGAN BENAR MELALUI AUDIOVISUAL DI TAMAN KANAK-KANAK Septiani Septiani; Dian Rachma Wijayanti; Gusti Kumala Dewi; Sabarina Elfrida Manik
JMM (Jurnal Masyarakat Mandiri) Vol 7, No 2 (2023): April
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v7i2.12875

Abstrak: Gigi dan mulut merupakan organ yang penting dalam pencernaan makanan. Kesehatan gigi merupakan hal yang penting terutama bagi anak-anak. Rusaknya gigi pada anak-anak sering diabaikan dan dianggap biasa. Edukasi ini bertujuan untuk meningkatkan pengetahuan anak-anak TK tentang kuman gigi, kuman mulut serta cara menyikat gigi dengan benar melalui audiovisual. Responden kegiatan berjumlah 41 anak-anak TK Al-Izhar Bekasi. Kegiatan edukasi dilakukan dengan menggunakan power point dan materi audiovisual. Pengukuran pengetahuan dilakukan menggunakan pre-test dan post-test. Analisis data menggunakan analisis deskriptif dan uji Wilcoxon signed rank. Terjadi peningkatan persentase menjawab dengan tepat pada setiap topik soal Persentase peningkatan nilai pre-test dan post-test sebanyak 17%, 12%, 15% dan 2%. Nilai akhir uji Wilcoxon menunjukkan nilai p value = 0.002. Nilai P < 0.005, maka dapat disimpulkan bahwa terdapat pengaruh edukasi kuman gigi dan mulut serta cara menyikat gigi dengan benar melalui audiovisual yang telah dilaksanakan terhadap pengetahuan siswa/I TK Al-Izhar. Abstract: Teeth and mouth are important organs in the digestion of food. Dental health is important, especially for children. Tooth decay in children is often overlooked and taken for granted. This education aims to increase kindergarten children's knowledge about tooth germs, mouth germs and how to brush their teeth properly through audiovisual. Respondents to the activity totaled 41 children of TK Al-Izhar Bekasi. Educational activities are carried out using power points and audiovisual materials. Measurement of knowledge is done using pre-test and post-test. Data analysis used descriptive analysis and Wilcoxon sisigned-rank test. There was an increase in the percentage of answering correctly on each topic. The percentage increase in pre-test and post-test scores was 17%, 12%, 15% and 2%. The final value of the Wilcoxon test shows a p-value 0.002. P-value <0.005, it can be concluded that there is an effect of education on dental and oral germs and how to brush teeth properly through audiovisual that has been carried out on the knowledge of students/I TK Al-Izhar.

Sensitivitas Pemeriksaan Covid-19: Insulated Isothermal PCR (iiPCR) Dan Reverse Transcription PCR (RT-PCR) Lisa Yunita; Dian Rachma Wijayanti; Apriani Riyanti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.12441

Covid-19 (Corona Virus disease 2019) is a new type of disease caused by a virus from the Corona Virus group, namely SARS-CoV-2. Covid-19 may cause respiratory system disorders, ranging from mild symptoms such as flu to lung infections, such as pneumonia. Laboratory diagnoses for Covid-19 disease generally include hematological (complete blood examinations), and molecular or a combination of serology and molecular. PCR examination that can be carried out using the Insulated Isothermal PCR and Reverse Transcription PCR methods. This research is descriptive observational research. Data were collected at Grha Kedoya Hospital, West Jakarta, North Kedoya. The result showed a sensitivity of iiPCR score 82,6%, which is relatively lower than gold-standard RT-PCR. Our research suggested that RT-PCR is still an effective and sensitive method for Covid-19 examination.

Co-Authors Achmadi Achmadi Afifah, Nazwa Pramitya Aidha Zulaika Ajeng Putri Edelweis Albustomi, Yazit Almatin Puspa Dewi ari ardiyanto sardi Arigayota Darwin Mahara Kala Astriana, Destia Aturut Yansen Ayu, Olga Dewi Citra Bara, Steffany Nurjanah Batu Cut Nur Masita Dhian Ekawati Dwitami, Ananda Rizki Emhari, Awaliyah Frida Octavia Purnomo Gusti Kumala Dewi Halimah Nurul Istiqomah Humaedi, Aji Indah Andriyanti Intan Kurniawati Pramitaningrum Lisa Yunita Lukitasari, Nurraya Mahara Kala, Arigayota Darwin Maman Turjaman Maman Turjaman Marini, Meta Nur'Izzu Marvianda, Delva Rouza Mimah Mulhimah Mohamad Syafaat Mohammad Maulana Fajar Muhammad Rizki Kurniawan Muhammad Rizki Kurniawan Muhammad Safaat Mulyatmo, Pravita Deasy Setyaningrum Nicolaus Sri Widada Nurhusna, Maryam Purboningrum, Diah Ayu Ritonga, Ahmad Fitra Riyadi, Sugeng Ahmad Riyanti, Apriani Sabarina Elfrida Manik Sabarina Elfrida Manik Sadwika Najmi Kautsari Sari, Anita Fatikah Sari, Puspa Puspita Septi, Septiani Septiani Septiani Septiani Septiani Sintya, Dinda Putri Wisnu Murti Wulan Fitriani Safari Yazit Albustomi Yoelianto, Yassmin Athaya Yunita, Lisa

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