Sammy Londong
FPIK UNSRAT

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BAKTERI RESISTEN MERKURI PADA ALGA COKLAT Padina australis DARI PERAIRAN KIMA BAJO SULAWESI UTARA Sri Winisari Van Gobel; Desy MH Mantiri; James JH Paulus; Rizald Max Rompas; Natalie D Rumampuk; Sammy Londong
JURNAL PESISIR DAN LAUT TROPIS Vol. 9 No. 3 (2021): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.3.2021.36522

Abstract

Alga laut jenis Padina australis merupakan spesies alga coklat divisi Phaeophyta (alga cokelat) yang umumnya tersebar di perairan laut. Spesies ini mampu bertahan hidup di perairan yang mempunyai kualitas air rendah. Tujuan penelitian ini adalah untuk mengisolasi bakteri resisten merkuri pada alga P. australis, mengetahui bentuk sel dan karakteristik bakteri. Metode yang digunakan adalah isolasi bakteri secara konvensional dan diuji pada senyawa merkuri klorida (HgCl2.) Hasil uji morfologi bakteri dari alga P. australis adalah Gram positif berbentuk monobasil dan streptobasil serta mempunyai kemampuan resisten terhadap merkuri pada konsentrasi 250, 500 dan 1000 ppm. Uji antibiotik dari bakteri resisten merkuri tersebut dilakukan terhadap antibiotik amoxicillin, cefixime dan doxycycline. Hasil menunjukan bahwa antibiotik amoxicillin mempunyai zona hambat 0,4 mm, antibiotik cefixime dengan zona hambat 0,1 mm. dan doxycycline mempunyai zona hambat tertinggi yaitu 1,8 mm, terlihat bahwa bakteri dapat mengurangi efektifitas suatu obat. Kata Kunci : Alga coklat, bakteri Gram positif, bakteri resisten merkuri, antibiotik
UJI AKTIVITAS ANTIOKSIDAN PADA ASTEROIDEA (Linckia laevigata) DARI PERAIRAN TONGKAINA KECAMATAN BUNAKEN KOTA MANADO Kristoper A Lidongi; Chatrien A.L. Sinjal; Kurniati Kemer; Remmy E.P. Mangindaan; Grevo Soleman Gerung; Sammy Londong
JURNAL PESISIR DAN LAUT TROPIS Vol. 9 No. 3 (2021): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.3.2021.38430

Abstract

Indonesia has abundant biological resources that produce many potential bioactive compounds that are useful for pharmaceuticals and other materials, one of which is antioxidants. Antioxidants are substances that can prevent or slow down cell damage caused by free radicals. This study aims to analyze how strong the antioxidant activity is in the body of asteroidea (Linckia laevigata) taken in Tongkaina waters, Bunaken District, Manado City. And this study found that the inhibition value of the ethanolic extract of Linckia laevigata had strong antioxidant activity where the extract increased and the concentration was 10 mg/L with an average value of 67,3 %, a concentration of 15 mg/L  of 67,8 %, a concentration of 20 mg/L of 68,8 % and the highest concentration of 25 mg/L getting an average value of 69 %. The increace in the percentage of inhibition and the ethanolic extract of Linckia laevigata, showed that the concentration of the extract contributed greatly to the ability of the extract to reduce free radicals. In this study, the DPPH test was carried out by observing the decrease in absorbance wavelength of 517 nm using UV-Vis spectrophotometer,where the decrease in absorbance value as a result and a decrease in color intensity. While vitamin C as a comparison has a greater yield and sample extract of Linckia laevigata which proves that the antioxidant content and the sample has a fairly stable result.Keywords: Bioaktif compounds, Linckia laevigata, Antioxidant, Vitamin C