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Effect of Extraction Method and Solvent Type on Total Phenolics Content, Total Flavonoid and Antioxidant Activity of Pegagan Extract (Centella asiatica (Linn.) Urban) Ahyar Riza; Syafruddin Ilyas; Endang Sjamsudin; Delyuzar Delyuzar
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 1 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i1.902

Background: Centella asiatica (CA) or pegagan or horse foot leaves is one of the traditional plants widely used to treat various diseases. This study aims to evaluated the content of total phenolics and total flavonoids, as well as the antioxidant activity of Centella asiatica extracts from different methods and solvents. Methods: The sample was divided into three groups, (70% ethanol maceration, methanol maceration and water infundation). Total phenolic compounds in the extract were determined by the Follin-Ciocalteu method, total flavonoid content was determined by the aluminum chloride colorimetric assay method, and antioxidant activity was assessed by the immersion DPPH free radicals method. Determination data of IC50 was assessed by regression test and differences between the three extract groups were tested by ANOVA or Kruskal Wallis test. Results: The highest total phenolics and total flavonoid were obtained in Centella asiatica extract with water solvent, 251.88 ± 0.96 mg/g GAE and 7.26 ± 0.03 mg/g QE, followed by 70% ethanol and methanol. In addition, the best antioxidant activity was also shown in the extract with water, namely 24.09 ± 0.01. The difference test results between all groups showed significant results (p<0.05) in all parameters. Conclusion: The best content of total phenolic, total flavonoid, and antioxidant activity was obtained by infundation with water solvent. In the maceration method, the best content of the three parameters measured was obtained in the group with ethanol solvent.

Investigation of 3% Binahong (Anredera cordifolia) Leaf Extract Nanogel on the Alveolar Bone Healing: BMP-2 Modulation in Rat Models Bernard Bernard; Syafruddin Ilyas; Olivia Avriyanti Hanafiah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 7 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i7.1020

Background: Alveolar bone healing relies on osteoblasts activity and differentiation, with bone morphogenetic protein-2 (BMP-2) playing a crucial regulatory role. Binahong leaf extract (BLE) has demonstrated efficacy in bone healing due to its rich phytochemical composition. Nanogel offers enhanced bioavailability and targeted release to effectively deliver therapeutic agents. This study aims to assess the impact of 3% BLE nanogel on BMP-2 expression in tooth extraction socket healing. Methods: Thirty male Wistar rat underwent anaesthesia for the extraction of their lower incisors to induce alveolar bone healing. Subjects were randomly assigned into treatment (BLE nanogel) and control (base nanogel) group. Five rats from each group were respectively sacrificed at 7, 14, and 28 days after procedures. BMP-2 expression was assessed by performing immunohistochemistry analysis using BMP antibody reagent. Data were analysed using chi-square. Results: The analysis of BMP-2 expression showed no significant difference between treatment and control groups on days 7, 14, and 28 (p>0,05). However, a subtle increase was observed in the treatment group throughout observation period. Conclusion: Application of 3% BLE nanogel may enhance the expression of BMP-2, even though it was not significantly increase. The findings underscore the complex interplay between Binahong leaf extract, nanogel and BMP-2 expression.

The Effect of Snakehead Fish (Channa striata) Extract on Dry Socket Wound Healing: TGF-β1 Expression in Rats Model Afifah Ayuni HG; Syafruddin Ilyas; Olivia Avriyanti Hanafiah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 7 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i7.1035

Background: Dry socket or alveolar osteitis is a condition characterized by the breakdown of blood clots due to excessive fibrinolysis activity, which disrupts the wound healing process. Consumption of nutrition-rich foods such as snakehead fish (Channa striata) rich in albumin, omega-3 fatty acids, vitamins, minerals, and striatin may accelerate delayed wound healing. This study aimed to analyze the role of snakehead fish extract on the expression of TGF-β1 in the healing of dry socket wounds after tooth extraction in Wistar rats. Methods: This study was an in vivo study with a posttest-only control group design. Forty-five Wistar rats were divided into five groups: snakehead fish extract groups with doses of 1/2, 1, 2, 4 times human dose, and a negative control. The lower left incisor teeth of the rats were extracted, then dry socket was induced with adrenaline using a paper point. On days 3, 7, and 14, TGF-β1 expression was assessed using immunohistochemical staining. Results: This study showed significant difference in TGF-β1 expression on days 3, 7, and 14. TGF-β1 expression was higher in the treatment groups than the control (p<0.05). Conclusion: Administration of snakehead fish extract increased TGF-β1 expression. These findings underline the complex interaction between snakehead fish extract and TGF-β1 expression.

Effect of Extraction Method and Solvent Type on Total Phenolics Content, Total Flavonoid and Antioxidant Activity of Pegagan Extract (Centella asiatica (Linn.) Urban) Ahyar Riza; Syafruddin Ilyas; Endang Sjamsudin; Delyuzar Delyuzar
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 1 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i1.902

Background: Centella asiatica (CA) or pegagan or horse foot leaves is one of the traditional plants widely used to treat various diseases. This study aims to evaluated the content of total phenolics and total flavonoids, as well as the antioxidant activity of Centella asiatica extracts from different methods and solvents. Methods: The sample was divided into three groups, (70% ethanol maceration, methanol maceration and water infundation). Total phenolic compounds in the extract were determined by the Follin-Ciocalteu method, total flavonoid content was determined by the aluminum chloride colorimetric assay method, and antioxidant activity was assessed by the immersion DPPH free radicals method. Determination data of IC50 was assessed by regression test and differences between the three extract groups were tested by ANOVA or Kruskal Wallis test. Results: The highest total phenolics and total flavonoid were obtained in Centella asiatica extract with water solvent, 251.88 ± 0.96 mg/g GAE and 7.26 ± 0.03 mg/g QE, followed by 70% ethanol and methanol. In addition, the best antioxidant activity was also shown in the extract with water, namely 24.09 ± 0.01. The difference test results between all groups showed significant results (p<0.05) in all parameters. Conclusion: The best content of total phenolic, total flavonoid, and antioxidant activity was obtained by infundation with water solvent. In the maceration method, the best content of the three parameters measured was obtained in the group with ethanol solvent.

The Effect of Snakehead Fish (Channa striata) Extract on Dry Socket Wound Healing: TGF-β1 Expression in Rats Model Afifah Ayuni HG; Syafruddin Ilyas; Olivia Avriyanti Hanafiah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 7 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i7.1035

Background: Dry socket or alveolar osteitis is a condition characterized by the breakdown of blood clots due to excessive fibrinolysis activity, which disrupts the wound healing process. Consumption of nutrition-rich foods such as snakehead fish (Channa striata) rich in albumin, omega-3 fatty acids, vitamins, minerals, and striatin may accelerate delayed wound healing. This study aimed to analyze the role of snakehead fish extract on the expression of TGF-β1 in the healing of dry socket wounds after tooth extraction in Wistar rats. Methods: This study was an in vivo study with a posttest-only control group design. Forty-five Wistar rats were divided into five groups: snakehead fish extract groups with doses of 1/2, 1, 2, 4 times human dose, and a negative control. The lower left incisor teeth of the rats were extracted, then dry socket was induced with adrenaline using a paper point. On days 3, 7, and 14, TGF-β1 expression was assessed using immunohistochemical staining. Results: This study showed significant difference in TGF-β1 expression on days 3, 7, and 14. TGF-β1 expression was higher in the treatment groups than the control (p<0.05). Conclusion: Administration of snakehead fish extract increased TGF-β1 expression. These findings underline the complex interaction between snakehead fish extract and TGF-β1 expression.

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