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Rudi Kartika
Jurusan Kimia, FMIPA, Universitas Mulawarman
Chemistry

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Pengujian Toksisitas dan Antioksidan Ekstrak Etilasetat dan n-Butanol Hipokotil Sarang Semut, Myrmecodia pendens Merr. & Perry Vino Soaduon Hamonangan Simanjuntak; Endah Wydiastuti; Lina Marlina; Partomouan Simanjuntak; Rudi Kartika
JURNAL KIMIA MULAWARMAN Vol 21 No 1 (2023)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v21i1.1288

Abstract

Sarang semut, Myrmecodia pendens Merr. & Perry (Rubiaceae) is an epiphytic plant from Papua which is used to cure various diseases. The purpose of this study is to see the chromatogram profile [Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC)] analysis, and biological test of ethyl acetate and n-butanol of Sarang semut’s hypocotyl extracts from methanol extracts. Meanwhile, the biological tests carried out were toxicity tests on Artemia salina L shrimp larvae and antioxidant assay with free radical scavenging methods (DPPH). The results of the antioxidant activity test showed that the antioxidant activity of the ethyl acetate extract was higher (IC50=18.23μg/ml) than the n-butanol extract (IC50=29.58μg/ml). The results of the toxicity test on the ethyl acetate extract were higher (LC50=101.84 g/ml) than the n-butanol extract (LC50=154.59 g/ml). TLC analysis results showed that the mobile phase for the best separation of chemical compounds in ethyl acetate extract was chloroform-methanol (10:1) and n-butanol extract was the mobile phase chloroform-methanol-water (5:5:1), while HPLC analysis showed that the mobile phase for the separation of chemical compounds in the ethyl acetate extract was n-hexane-ethyl acetate (10:1) and the n-butanol extract was methanol-water (10:1).
Fermentasi Singkong Karet (Monihot carthagenensis MÜII) menjadi Etanol dengan Pemanfaatan Albumin Dari Ikan Gabus (Channa striata) Sebagai Sumber Nitrogen Bagi Mikroba Saputri Satriyana Widyaningrum; Rudi Kartika; Erwin Erwin
JURNAL KIMIA MULAWARMAN Vol 19 No 2 (2022)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v19i2.521

Abstract

Has been cassava rubber fermentation (M. carthagenensisMÜll) as a raw material for making ethanol with the utilization of fishcork albumin (Channastriata) as nitrogen Saccharomyces cerrevisiae with enzymatic hydrolysis result through 2 stages ieliquification using α-amylase and saccharification stage using gluco -amilase. The best reducing sugar content was obtained at 0.1307%. Based on this research, maximum ethanol content was obtained with 0.5% concentration of nutrient at fermentation time for 232 hours, obtained by result of 12,688% at 5.6 mL distillate volume using gas chromatography method.
Co-Authors Endah Wydiastuti Erwin Erwin Lina Marlina Partomouan Simanjuntak Saputri Satriyana Widyaningrum Vino Soaduon Hamonangan Simanjuntak