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Feronica, Donna
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Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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PENGARUH VARIASI LAMA WAKTU EKSTRAKSI TERHADAP FLAVONOID TOTAL DAUN TAPAK LIMAN ( Elephantopus scaber L.) SECARA SPEKTROFOTOMETRI UV-Vis Feronica, Donna; Susilowati, Susilowati; Saad, Muhammad
Jurnal Farmasi (Journal of Pharmacy) Vol 13, No 1 (2024)
Publisher : SEKOLAH TINGGI ILMU KESEHATAN NASIONAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37013/jf.v13i1.279

Abstract

Tapak Liman (Elephantopus scaber L.) merupakan salah satu tumbuhan yang  dapat dimanfaatkan sebagai obat tradisional dengan kandungan flavonoid. Penelitian ini bertujuan untuk mengetahui jumlah kadar flavonoid total  dari daun tapak liman dengan variasi lama waktu perebusan 5 menit, 10 menit  dan 15 menit. Daun tapak liman kering diekstraksi menggunakan air dengan pemanasan pada suhu 95C selama 5, 10 dan 15 menit. Metode Identifikasi senyawa flavonoid pada hasil rebusan daun tapak liman dengan uji metode Wilstater dan uji reagen alkali. Pengukuran flavonoid total menggunakan metode Spektrofotometri UV-Vis yang dihitung sebagai kuersetin. Hasil penelitian menunjukkan seluruh sampel uji mengandung flavonoid. Kadar flavonoid total daun tapak liman tertinggi diperoleh pada menit ke 5 menit sebesar 94,59 ± 0,1424  mgQE/ 100 ml  dan terendah pada menit ke 15 menit sebesar 85,55 ± 0,2376 mgQE/ 100ml. Pada uji “One Way Anova” nilai signifikansi pada kadar flavonoid sebesar 0,006 < 0,05 sehingga dapat disimpulkan lama waktu pemanasan berpengaruh signifikan terhadap flavonoid total dalam daun tapak liman
PENGARUH VARIASI LAMA WAKTU EKSTRAKSI TERHADAP FLAVONOID TOTAL DAUN TAPAK LIMAN (Elephantopus scaber L.) SECARA SPEKTROFOTOMETRI UV-Vis Feronica, Donna; Susilowati; Saad, Muhammad
Jurnal Farmasi (Journal of Pharmacy) Vol. 13 No. 1 (2024): Jurnal Farmasi (Journal of Pharmacy), April 2024
Publisher : Sekolah Tinggi Ilmu Kesehatan Nasional & Pengurus Cabang Ikatan Apoteker Indonesia (IAI) Sukoharjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.373013/3a14k719

Abstract

Elephantopus scaber L. is used as a traditional medicine with flavonoid content. This study aims to determine the total flavonoid content of Elephantopus scaber leaves with varying extraction times of 5 minutes, 10 minutes and 15 minutes. Dried Elephantopus scaber leaves were extracted using water by heating at 95oC for 5, 10 and 15 minutes. Method: Identification of flavonoid compounds in the boiled Elephantopus scaber leaves used the Wilstater method and alkaline reagent test. Total flavonoids were measured using the UV - Vis Spectrophotometry method as quercetin. The research results showed that all test samples contained flavonoids. The highest total flavonoid content of Elephantopus scaber leaves was obtained at the 5th minute, amounting to 94.59 ± 0.1424 mgQE/ 100 ml and the lowest at the 15th minute, amounting to 85.55 ± 0.2376 mgQE/ 100ml. In the "One Way Anova" test, the significance value for flavonoid levels was 0.006 < 0.05, so it can be concluded that the length of heating time had a significant effect on the total flavonoids in Elephantopus scaber leaves. The recommended boiling time for tapak Liman leaves is 5 minutes at 95oC.
Co-Authors Muhammad Sa'ad Muhammad Saad, Muhammad Susilowati Susilowati Susilowati