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All Journal Jurnal Kesehatan Rajawali Indonesian Research Journal on Education
Susilawati, Anissa
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Optimasi Variasi Konsentrasi, Waktu Sentrifugasi Polyethylene Glycol (PEG), dan Modified Egg Yolk Lipemic Serum pada Enzim Serum Glutamic Pyruvic Transaminase (SGPT) Susilawati, Anissa; Riyani, Ani
Jurnal Kesehatan Rajawali Vol 13 No 2 (2023): Jurnal Kesehatan Rajawali
Publisher : Sekolah Tinggi Ilmu Kesehatan Rajawali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54350/jkr.v13i2.198

Abstract

Examination of Serum Glutamic Pyruvic Transaminase (SGPT) enzymes can be disrupted due to the presence of fatty serum. The addition of Polyethylene Glycol (PEG) to fatty serum will lower the surface tension of the serum so that the fat will precipitate and eliminate error readings on the photometer. This study aims to determine the concentration and centrifugation time of the addition of Polyethylene Glycol (PEG) under optimal conditions. Serum modification was made to resemble fatty serum using egg yolks with three variations of triglyceride levels in ±500 mg/dL, ±600 mg/dL, ±700 mg/dL. Fatty serum is made using three concentrations of Polyethylene Glycol (PEG), namely 0.05%, 0.10%, 0.15%. Then incubated for 30 minutes at 40C and precipitation was carried out using centrifugation at 3000 rpm with three variations of centrifugation time, namely at 5, 10 and 15 minutes. Then the resulting supernatant was separated and measured to determine the Serum Glutamic Pyruvic Transaminase (SGPT) enzyme using a photometer. Then the data were analyzed using a two way ANOVA test followed by a Post Hoc test with a 95% confidence level. The conclusion of this study is that at 500 mg/dL fatty serum, the optimal concentration was obtained, namely 0.10% with a 10-minute centrifugation time, at 600 mg/dL fatty serum, the optimal concentration was obtained, namely 0.10% with a 10-minute centrifugation time, on fatty serum 700 mg/dL obtained the optimal PEG concentration and centrifugation time of 0.15% with a centrifugation time of 15 minutes.
Evaluasi Sediaan dan Uji Koefisien Fenol Disinfektan Pembersih Lantai pada Produk X dengan Kandungan Benzalkonium Klorida Maharani, Sherin Anindhia; Prabu, Ginanjar; Susilawati, Anissa; Andini, Risty
Indonesian Research Journal on Education Vol. 5 No. 4 (2025): Irje 2025
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/irje.v5i4.3111

Abstract

Infeksi nosokomial merupakan salah satu masalah kesehatan masyarakat yang serius, terutama yang disebabkan oleh Staphylococcus aureus. Tingginya prevalensi infeksi ini mendorong perlunya disinfektan yang efektif untuk menjaga kebersihan lingkungan, terutama di rumah sakit. Tujuan dari penelitian ini untuk mengevaluasi efektivitas antibakteri dari disinfektan pembersih lantai yang mengandung benzalkonium klorida menggunakan metode uji koefisien fenol. Penelitian ini dilakukan melalui pengujian mengenai uji organoleptik, uji pH, serta analisis homogenitas, viskositas produk dan koefisien fenol. Pengujian dilakukan untuk memastikan kualitas fisik dan kimia dari sediaan serta untuk menilai kemampuan disinfektan dalam membunuh Staphylococcus aureus. Hasil penelitian menunjukkan bahwa disinfektan pembersih lantai produk tersebut memenuhi standar dalam uji organoleptik, pH, homogenitas, dan viskositas, menunjukkan bahwa produk ini stabil dan efektif. Produk tersebut juga memiliki nilai koefisien fenol yang signifikan, lebih tinggi dibandingkan produk sejenis di pasaran. Disinfektan pembersih lantai yang diuji dapat direkomendasikan sebagai produk efektif dalam sanitasi untuk mengurangi risiko infeksi nosokomial di lingkungan kesehatan.
Co-Authors Andini, Risty Maharani, Sherin Anindhia Prabu, Ginanjar Riyani, Ani