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All Journal Journal of Educational Innovation and Public Health Detector: Jurnal Inovasi Riset Ilmu Kesehatan
Isna Nur Khasanah
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Dentistry Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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Uji Aktivitas Antioksidan Ekstrak dan Fraksi Buah Jambu Biji Australia (Psidium Guajava L.) Metode DPPH Marshela Duta Larasati; Desy Ayu Irma Permatasari; Isna Nur Khasanah
Journal of Educational Innovation and Public Health Vol. 1 No. 4 (2023): Oktober : Journal of Educational Innovation and Public Health
Publisher : Pusat Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/innovation.v1i4.1878

Abstract

Australian guava fruit (Psidium guajava L.) is thought to have antioxidant activity. Antioxidants are compounds that are useful for treating oxidative damage caused by free radicals in the body. The aim of this study was to determine the secondary metabolite content and IC50 value on the antioxidant activity of extracts and fractions from Australian guava fruit. This research used four solvents, including 70% ethanol extract, n-hexane fraction, ethyl acetate fraction and water fraction with varying concentrations of 50 ppm, 100 ppm, 150 ppm, 200 ppm and 250 ppm. The antioxidant test was carried out using a UV-Vis spectrophotometry tool with the DPPH method at a wavelength of 515 nm with an incubation time of 15 minutes. Antioxidant activity tests show that Australian guava fruit is effective as an antioxidant in capturing free radicals. The IC50 value for the ethanol extract is 0.784 µg/mL, the n-hexane fraction is 2.448 µg/mL, the ethyl acetate fraction is 1.015 µg/mL and the water fraction is 0.676 µg/mL. All samples were included in the very strong category, but the water fraction had the best antioxidant activity among the ethanol extract, n-hexane and ethyl acetate fractions.
Uji Aktivitas Antioksidan Ekstrak dan Fraksi n-Heksan, Etil asetat, dan Air Kulit Jambu Biji Australia (Psidium guajava L.) Dengan Metode DPPH (1,1-Difenil-2-Pikrilhidrazil) Ning Rusmiyati; Desy Ayu Irma Permatasari; Isna Nur Khasanah
Detector: Jurnal Inovasi Riset Ilmu Kesehatan Vol. 1 No. 4 (2023): November : Jurnal Inovasi Riset Ilmu Kesehatan
Publisher : Pusat Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/detector.v1i4.2553

Abstract

Free radicals work by binding to molecules or cells in the body and are very dangerous because they can damage cells in the body and trigger disease. Antioxidants are compounds that can counteract the effects of free radicals. This study aims to determine the antioxidant activity of ethanol extract and the n-hexane, ethyl-acetate and water fractions of Australian guava peel (Psidium guajava L.). Extraction was carried out by maceration method using 70% ethanol. The skin of the Australian guava (Psidium guajava L.) contains alkaloids, flavonoids, tannins, triterpenoids and saponins. Fractionation was carried out with solvents based on polarity differences obtained by the n-hexane fraction, ethyl acetate fraction, and air fraction. Antioxidant test by inhibiting DPPH free radicals (1,1-Diphenyl-2-Picrylhydrazyl) on ethanol extract and n-hexane fraction, ethyl acetate fraction and air at several concentrations, namely 50 ppm, 100 ppm, 150 ppm, 200 ppm, and 250 ppm. The IC50 calculation results obtained for the ethanol extract were 4.406 µg/mL, the n-hexane fraction was 4.131 µg/mL, the ethyl acetate fraction was 3.812 µg/mL and the air fraction was 3.312 µg/mL. The antioxidant activity with the best IC50 value was the fraction air.
Co-Authors Desy Ayu Irma Permatasari Marshela Duta Larasati Ning Rusmiyati