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Eksplorasi Tumbuhan Berkhasiat Obat di Bantaran Sungai Brantas Sebagai Upaya Konservasi Sungai Ramadhani, Dwi Nova; Asmiatin, Fitriah
Environmental Pollution Journal Vol. 2 No. 1: April 2022
Publisher : ECOTON: Ecological Observation and Wetlands Conservation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58954/epj.v2i1.40

Abstract

Sungai Brantas merupakan sungai terpanjang kedua setelah sungai Bengawan Solo di Jawa Timur. Kawasan Daerah Aliran Sungai Brantas memiliki biodiversitas yang cukup tinggi, vegetasi tumbuhannya pun melimpah, salah satunya yakni tumbuhan yang berkhasiat obat. Tujuan dari penelitian ini untuk mengetahui jenis-jenis tumbuhan berpotensi memiliki khasiat obat di bantaran Sungai Brantas sebagai upaya konservasi bantaran sungai. Pengambilan sampel dilakukan dengan menggunakan metode purposive random sampling dengan menggunakan 4 titik lokasi (Jatigedong, Randuwatang, Pasinan, dan Wringinanom). Pengamatan spesies menggunakan metode deskriptif observatif yang melakukan pengamatan langsung di lapangan. Hasil penelitian menunjukkan bahwa sebanyak 27 famili dengan 40 spesies tumbuhan berkhasiat obat ditemukan pada bantaran Sungai Brantas Jawa Timur, famili Asteraceae merupakan famili dengan spesies paling banyak. Tumbuhan-tumbuhan tersebut tidak hanya memiliki fungsi secara morfologi, namun juga memiliki khasiat obat.
The Effect of IBA and NAA on Root Induction of Tithonia diversifolia (Hemsl.) Ramadhani, Dwi Nova; Hidayati, Irul; Faizah, Hanik
Biotropika: Journal of Tropical Biology Vol. 13 No. 3 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.03.06

Abstract

Tithonia diversifolia (Hemsl.) is a plant that has a lot of potential for use in several sectors, so that large-scale and short-time propagation efforts are needed. Tissue culture is an appropriate technique for cultivating Tithonia diversifolia plants. The success of root induction in tissue culture is largely determined by the auxin growth regulator. This study aimed to determine the effect of IBA and NAA on root induction of T. diversifolia plants. The method was an experimental study with a Completely Randomised Design (CRD). Tithonia diversifolia plant node explants were planted into Murashige and Skoog (MS) media supplemented with growth regulators IBA (1 and 2 mg/L), NAA (1 and 2 mg/L), and a combination of IBA and NAA (1 mg/L + 1 mg/L; 1 mg/L + 2 mg/L; 2 mg/L + 1 mg/L; and 2 mg/L + 2 mg/L) and control group, consisting of explants cultured on a hormone-free medium which were then incubated for 4 weeks. The observation data were analysed statistically using the non-parametric Kruskal-Wallis test, followed by the Mann-Whitney test. The results showed that all parameters significantly affected auxins IBA and NAA in the root induction of T. diversifolia plants. The NAA 2 ppm treatment was the most effective in terms of root emergence time (3.67 DAP). Additionally, the combination of IBA and NAA was the optimal treatment in terms of root growth percentage (1.00%). The IBA 2 ppm + NAA 2 ppm treatment yielded the best results in terms of root number (10.17 strands). The NAA 1 ppm treatment yielded the best results in terms of root length (11.3 cm), root fresh weight (0.0967 g), and root dry weight (0.0144 g).