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UJI AKTIVITAS ANTIOKSIDAN EKSTRAK BAWANG HITAM (BLACK GARLIC) DENGAN VARIASI LAMA PEMANASAN Agustina, Eva; Andiarna, Funsu; Hidayati, Irul
Al-Kauniyah: Jurnal Biologi Vol 13, No 1 (2020): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

AbstrakSenyawa radikal dalam tubuh sangat reaktif terhadap sel-sel, sehingga dapat menyebabkan berbagai penyakit. Senyawa yang mampu menangkal radikal bebas disebut antioksidan. Bawang hitam diketahui mengandung senyawa aktif fenolik dan turunannya sehingga dapat digunakan sebagai antioksidan. Penelitian tentang penggunaan bawang hitam sebagai antioksidan ini bertujuan untuk mengetahui perbedaan waktu pemanasan (15, 25, dan 35 hari) terhadap aktivitas antioksidan pada ekstrak bawang hitam. Bawang hitam diperoleh dengan cara memanaskan bawang putih selama 15, 25, dan 35 hari. Bawang hitam selanjutnya diekstraksi dengan pelarut metanol dengan metode maserasi. Filtrat hasil ekstraksi dipekatkan dengan rotary evaporator untuk mendapatkan ekstrak bawang hitam. Ekstrak bawang hitam diuji fitokimia untuk mengetahui kandungan senyawa aktif. Aktivitas antioksidan ekstrak bawang hitam ditentukan dengan metode penangkalan radikal 2,2-diphenyl-1-picrylhydrazyl (DPPH). Hasilnya menunjukkan ekstrak bawang hitam memiliki aktivitas antioksidan yang sangat kuat dengan nilai IC50 masing-masing 15 hari yaitu 2,41 Âµg/mL; 25 hari yaitu 2,93 Âµg/mL; 35 hari yaitu 2,27 Âµg/mL. Nilai IC50 <10 Î¼g/mL menunjukkan bahwa ekstrak bawang hitam dengan waktu pemanasan 15, 25, dan 35 hari memiliki potensi antioksidan yang sangat kuat. Waktu pemanasan yang paling optimum dalam penangkalan radikal bebas adalah ekstrak bawang hitam dalam waktu pemanasan 35 hari, karena banyak terdapat kandungan senyawa flavonoid, tanin, sterol, dan saponin.Abstract Radical compounds in the body are very reactive to cells that can cause various diseases. Compounds that are able to ward off free radicals are called antioxidants. Black garlic are known to contain phenolic active compounds and their derivatives so that they can be used as antioxidants. Research on the use of black garlic as an antioxidant aims to determine the difference in heating time (15, 25, and 35 days) on the antioxidant activity of black garlic extract. Black garlic are obtained by heating the garlic for 15, 25, and 35 days. The black garlic is then extracted with methanol solvent by maceration method. The extracted filtrate was concentrated with a rotary evaporator to obtain the black garlic extract. Black garlic extract was tested for phytochemistry to determine the content of active compounds. The antioxidant activity of black garlic extract was determined by the radical deterrence method 2,2-diphenyl-1-picrylhydrazyl (DPPH). The results showed that the onion extract had a very strong antioxidant activity with an IC50 value of 15 days each of 2.41 Âµg/mL; 25 days which is 2.93 Î¼g/mL; 35 days which is 2.27 Âµg/mL. IC50 values <10 Î¼g/mL indicate that the black garlic extract with a heating time of 15, 25, and 35 days has a very strong antioxidant potential. The most optimum heating time in deterring free radicals is black garlic extract within 35 days of heating because there are many compounds containing flavonoids, tannins, sterols, and saponins.

Pendidikan Kesehatan tentang Penggunaan Antibiotik secara Tepat dan Efektif sebagai Upaya Mengatasi Resistensi Obat Andiarna, Funsu; Hidayati, Irul; Agustina, Eva
Journal of Community Engagement and Empowerment Vol 2, No 1 (2020)
Publisher : Institut Ilmu Kesehatah Bhakti Wiyata Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar

Antibiotik merupakan obat yang digunakan untuk mengobati infeksi bakteri. Penggunaan antibiotik yang tidak tepat, bahkan berlebihan dan dalam jangka waktu panjang menyebabkan terjadinya resistensi antibiotik. Dimana resistensi ini akan menyebabkan tidak terhambatnya pertumbuhan bakteri dengan pemberian antibiotik secara sistemik dengan dosis normal. Tujuan pelaksanaan kegiatan pengabdian masyarakat ini adalah memberikan pendidikan kesehatan tentang pengertian, penggunaan, dan akibat dari pemakaian antibiotik yang tidak bijak. Sasaran kegiatan ini adalah mahasiswa UIN Sunan Ampel Surabaya, dimana mahasiswa sering mengeluh beberapa penyakit ringan akibat dari bakteri seperti diare, sakit gigi, wajah berjerawat dan lain-lain. Metode pelaksanaan pengabdian masyarakat ini adalah dengan dilakukan pretest, sosialisasi dan posttest. Pretest dengan cara mahasiswa target menjawab pertanyaan di google form tentang pengetahuan awal terkait antibiotik; sosialisasi dengan metode pemberian pendidikan kesehatan (penyuluhan), diskusi dan tanya jawab. Selanjutnya dilakukan posttest dengan menjawab pertanyaan di google form sebagai evaluasi keberhasilan kegiatan. Hasil kegiatan pengabdian menunjukkan bahwa hasil pretest pada tingkat pengetahuan rendah sebesar 60,8%, tingkat pengetahuan sedang sebesar 34,2% dan tingkat pengetahuan tinggi sebesar 6%.Â Sedangkan pada hasil posttest didapatkan bahwa tingkat pengetahuan rendah sebesar 6,7%, tingkat pengetahuan sedang sebesar 36,6% dan tingkat pengetahuan tinggi sebesar 56,7%. Kesimpulan dari pelaksanaan kegiatan pengabdian masyarakat bahwa terdapat perubahan yang signifikan tingkat pengetahuan yang rendah dan tinggi, baik sebelum dan sesudah pendidikan Kesehatan. Hal ini menunjukkan mahasiswa telah memahami penggunaan antibiotik dengan tepat dan efektif.

ANALISIS PROKSIMAT PADA TEPUNG BIJI NANGKA (Artocarpus Heterophyllus Lamk.) ESTI NOVI ANDYARINI; IRUL HIDAYATI
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 1, No 1 (2017): Klorofil: Jurnal Ilmu Biologi dan Terapan
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v1i1.1239

Indonesia is a tropical country which is diverse types of plants, one of them which was plant jackfruit (Artocarpus heterophyllus Lamk.). But so far, its utilization of waste still leaves form the seeds of jackfruit. Basic human needs of food and technology progress encourages humans to cultivate seeds into flour, jackfruit seed jackfruit. To understand the nutrient content of quality food grain powder jackfruit is the researchers want to do an analysis of proksimat on seed flour, jackfruit. This research use experimental design to test the levels of protein, carbs, fat and calories jackfruit seed flour. The measurement of protein content by Kjeldahl method, method of Carbohydrate carbs by Defference method with Soklet,fat,and calories with manual calculation. Research results from the jackfruit seeds have been processed into flour, jackfruit seeds showed that levels of the protein in the flour biji nangka of 12.19 grams, carbohydrate levels of 56.21 grams, 1.12 grams of fat content and moisture content of 12.4 grams. Expected with the known value of jackfruit seeds flour on proksimat this will encourage the public to make use of waste food into jackfruit seeds in the form of jackfruit seed flour. In addition to reducing waste, nutrient content of jackfruit seed flour is also high.

Analisis Kadar Glukosa, Hemoglobin, dan Kolesterol Mencit (Mus Musculus) Setelah Diinduksi Aspartam Esti Tyastirin; Risa Purnamasari; Irul Hidayati; Eva Agustina; Moch. Irfan Hadi; Nova Lusiana; Mei Lina Fitri Kumalasari
Biotropic : The Journal of Tropical Biology Vol. 2 No. 2 (2018): Biotropic, Volume 2, Nomor 2, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Aspartame has a lower calorie rating than other sweeteners. for example, cyclamate, lactose, saccharin, fructose and maltosa. Although it has low calorie, aspartame have 60 times sweeter than sugar (sucrose). Induction of aspartame can cause elevated levels of blood glucose and damage to the pancreatic β cells of mice. The purpose of this study was to analyze the dosing of aspartame levels; 13 mg/Kg body weight, 39 mg/Kg and 78 mg/Kg and those effect to the levels of glucose, cholesterol and haemoglobin of female mice (Mus musculus). The methode of research laboratory experiments using a complete randomized design (RAL). This study was using 24 mice with 4 treatment groups. Mice were given aspartame during surgical treatment, mice were made to perform measurement of Hb levels, glucose levels and cholesterol levels of neonatal mice. Data analysis using the Kruskal Wallis because Gaussian data but not homogeneous. The results showed an increase in the levels of glucose and cholesterol levels, but statistically there is no difference in glucose levels (p = 0,087), Hb (p = 0,899) and cholesterol (p = 0,168) in mice (Mus musculus) who were given variations of the dose of aspartame.

Pengelompokan isolat bakteri penghasil hormon IAA (indole acetic acid) dari tanah rhizosfer bawang merah (Allium cepa) di Nganjuk dengan variasi wilayah yang berbeda Wuri Handayani; Misbakhul Munir; Irul Hidayati
Prosiding Seminar Biologi Vol 6 No 1 (2020): PROSIDING SEMINAR NASIONAL BIOLOGI DI ERA PANDEMI COVID-19 (OKTOBER 2020)
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v6i1.15787

Proses pertanian di Indonesia masih bergantung pada pemakaian pupuk anorganik atau pupuk kimia, khususnya di Nganjuk masih menggunakan pupuk kimia dalam proses budidaya tanaman bawang merah. Oleh karena itu, perlu diatasi dengan mengganti pupuk kimia dengan pupuk organik (biofertilizer) yang sangat aman dan ramah terhadap lingkungan karena mampu memperbaiki struktur tanah dan meningkatkan biomassa mikroba. Penelitian ini bertujuan untuk mengisolasi dan menguji kemampuan bakteri rizosfer dalam menghasilkan hormon IAA dengan jenis data kualitatif dan kuantitatif terhadap bakteri penghasil hormon IAA. Data yang diperoleh dianalisis secara statistik menggunakan uji Kruskal Wallis untuk membandingkan variasi dari tiga wilayah yang berbeda. Sampel tanah diisolasi dan dimurnikan di media NA. Hasil penelitian menunjukkan bahwa terdapat 41 isolat bakteri rizosfer bawang merah yang mampu menghasilkan hormon IAA dengan kadar konsentrasi tertinggi 31,634 ppm yang terdapat di wilayah L (jauh dari permukiman warga) dan konsentrasi terendah 2,131 ppm terletak di wilayah A (dekat permukiman warga). Hasil uji statistik menunjukkan bahwa tidak adanya perbedaan yang signifikan konsentrasi hormon IAA yang dihasilkan isolat bakteri rizosfer bawang merah dari berbagai wilayah tersebut menandakan bahwa kondisi dan komposisi tanah di sekitar wilayah tersebut tidak berbeda karena berada dalam satu lokasi persawahan di Dusun Jetis, Desa Kendalrejo, Kecamatan Bagor, Kabupaten Nganjuk.

Optimization of PCR Protocol for the Identification of Yeasts Isolated from Apis mellifera Honeycomb based on the ITS rDNA Ayudya Fitri Arifa; Nirmala Fitria Firdhausi; Irul Hidayati; Yuanita Rachmawati; Moch. Irfan Hadi
Biota Vol 8 No 2 (2022): Jurnal Biota 2022
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v8i2.10181

Yeast is a microorganism that can be found in honeycomb. Yeast identification is a process to find and identify new species. One of which is molecular identification of yeast with rDNA sequences in the ITS region. Before carrying out molecular identification, it is necessary to optimize yeast DNA amplification methods to obtain good DNA sequences that ease the yeast identification process. The purpose of this study was to discover the optimum PCR (Polymerase Chain Reaction)protocols for the identification of yeasts isolated from Apis mellifera honeycomb based on the ITS rDNA. This study used 3 PCR (Polymerase Chain Reaction) protocols, i.e., from Kanti et al. (2018), Ediningsari (2008), and Maulana (2011). This results study shows that the optimum PCR protocol was from Maulana (2011), which produced clear and whole DNA fragment luminescences.

Uji aktivitas antijamur ekstrak black garlic terhadap pertumbuhan jamur Candida albicans Eva Agustina; Funsu Andiarna; Irul Hidayati; Vindi Fristy Kartika
Bioma : Jurnal Ilmiah Biologi Vol. 10 No. 2: Oktober 2021
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v10i2.6371

Salah satu tanaman yang berfungsi sebagai antijamur adalah bawang putih yang diinovasikan menjadi produk bawang yang berwarna hitam atau black garlic. Penelitian ini bertujuan untuk mengetahui aktivitas antijamur ekstrak black barlic terhadap pertumbuhan jamur Candida albicans. Metoda dalam penelitian ini adalah bawang putih dipanaskan selama 35 hari pada suhu 65°C untuk mendapatkan black garlic. Black garlic diekstraksi dengan metode maserasi menggunakan pelarut methanol. Uji aktivitas antibakteri berdasarkan uji zona hambat, konsentrasi hambat minimal (KHM) dan konsentrasi bunuh minimal (KBM). Uji zona hambat dengan variasi konsentrasi ekstrak 100%, 90% dan 80%. Konsentrasi dengan nilai zona hambat yang baik akan dilanjutkan ke uji KHM dan KBM dengan pengenceran ke 1 sampai 5. Hasil penelitian menunjukkan bahwa zona hambat ekstrak Black Garlic dengan konsentrasi 100% memiliki zona hambat paling bagus yaitu 3,15 mm. Uji KHM optimum diperoleh pada pengenceran ke-5 dengan nilai OD terendah 0,460. Uji KBM dari ekstrak black garlic pada pengenceran ke-5 menunjukkan koloni jamur C. albicans masih tumbuh, sehingga dapat dikatakan bahwa ekstrak black garlic hanya mampu menghambat pertumbuhan jamur namun tidak bisa membunuh jamur C. albicans. Kata kunci: antijamur; black garlic; maserasi ABSTRACTActivity test of black garlic extract against the growth of the fungi Candida albicans. One plant that functions as an antifungal is garlic which is innovated into black garlic. The aim of this study is to indentify black garlic extract antifungal activity against of the Candida albicans. The method in this research was heated garlic for 35 days at temperature of 65°C. Black garlic was extracted by maceration method using methanol solvent. Antibacterial activity test based on inhibition zone test, minimal inhibitory concentration (MIC) and minimal kill concentration (MBC). Inhibition zone test with various extract concentrations of 100%, 90% and 80%. Concentrations with good inhibition zone values will be continued to the MIC and MBC tests with dilutions 1 to 5. The results showed that the inhibition zone of black garlic extract with a concentration of 100%, 3.15 mm. The optimum MIC test was obtained at the 5 dilution with the lowest OD value of absorbance 0.460. The MBC test of the black garlic extract at the 5 dilution showed C. albicans fungal colonies were still growing, so it can be said that the black garlic extract was only able to inhibit fungal growth but could not kill C. albicans fungi. Keywords: antifungal; black garlic; maceration

The Relationship Between The Number of Parities and Pregnancy Age with Maternal Anemia Irul Hidayati; Esti Novi Andyarini
Journal of Health Science and Prevention Vol. 2 No. 1 (2018): JHSP Vol 2 No 1 - 2018
Publisher : State Islamic University of Sunan Ampel

According to the World Health Organization, the anemia prevalence was estimated reach 9% in developed countries, while in developing countries reached 43%. The most-at-risk groups were children and women at fertile age, with an estimated prevalence of anemia in infants at 47%, pregnant women by 42%, and in non-pregnant women t ages 15-49 reached 30%. This was an observational analytic research with cross sectional approach. The purpose was to analyzed the relationship between the number of parity and the pregnancy age with the incidence of maternal anemia. The population in this study were 111 pregnant womens who checked their pregnancy to Public Healt Center (PHC) Kintamani 1 at Bangli regency, the province of Bali. The simple random sampling was used in the research with 87 peoples acted as research sample. Using Rank Spearman Correllation test, it was founded that there was a relationship between the number of parity with the incidence of maternal anemia a low relationship, and there was a relationship between gestational age with maternal anemia incidence with a low degree relationship.

ANALISIS PROKSIMAT PADA TEPUNG BIJI NANGKA (Artocarpus Heterophyllus Lamk.) ESTI NOVI ANDYARINI; IRUL HIDAYATI
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 1, No 1 (2017): Klorofil: Jurnal Ilmu Biologi dan Terapan
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v1i1.1239

Indonesia is a tropical country which is diverse types of plants, one of them which was plant jackfruit (Artocarpus heterophyllus Lamk.). But so far, its utilization of waste still leaves form the seeds of jackfruit. Basic human needs of food and technology progress encourages humans to cultivate seeds into flour, jackfruit seed jackfruit. To understand the nutrient content of quality food grain powder jackfruit is the researchers want to do an analysis of proksimat on seed flour, jackfruit. This research use experimental design to test the levels of protein, carbs, fat and calories jackfruit seed flour. The measurement of protein content by Kjeldahl method, method of Carbohydrate carbs by Defference method with Soklet,fat,and calories with manual calculation. Research results from the jackfruit seeds have been processed into flour, jackfruit seeds showed that levels of the protein in the flour biji nangka of 12.19 grams, carbohydrate levels of 56.21 grams, 1.12 grams of fat content and moisture content of 12.4 grams. Expected with the known value of jackfruit seeds flour on proksimat this will encourage the public to make use of waste food into jackfruit seeds in the form of jackfruit seed flour. In addition to reducing waste, nutrient content of jackfruit seed flour is also high.

Uji Prebiotik Mangga Manalagi (Mangifera indica L. var manalagi) Terhadap Pertumbuhan Lactobacillus plantarum Secara In Vitro Iit Lusif Tsania; Irul Hidayati; Ita Ainun Jariyah
JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Vol 6, No 2 (2021)
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/sst.v6i2.823

Prebiotics are food ingredients that cannot be absorbed by the host and will be fermented by colonic bacteria into Short-Chain Fatty Acid. Prebiotics can come from various natural ingredients such as wheat, onions, and bananas. Prebiotic food sources can stimulate the growth of probiotic bacteria which are beneficial bacteria for the host, one of which can produce bacteriocins to fight pathogenic bacteria. Manalagi mango (Mangifera indica L. var manalagi) is a fruit that has the potential as a source of prebiotics because it contains prebiotic food requirements, including dietary fiber and carbohydrates. The purpose of this study is to find out the ability or potential of Manalagi mengkal mango as a source of prebiotics by using a prebiotic test based on the growth of BAL is Lactobacillus plantarum in vitro. The design of this study used an experimental laboratory carried out in vitro by making carbon source media with various concentrations of mango flour, then mixed with liquid MRS, and given Lactobacillus plantarum culture which then measured the carbon source medium in the form of TPC. The highest TPC average yield was 1,7 x 1010 CFU/ml is the variation in the concentration of mango flour 6.89%. Based on the results of a prebiotic test with TPC mango flour manalagi mengkal showed its ability as a source of prebiotics by stimulating the growth of BAL (Lactobacillus plantarum).Keywords – Prebiotics, Manalagi mango, Lactobacillus plantarum

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