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Mulyawan, I Made
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Profil Pasien Kanker Kolorektal di RSUP Sanglah Tahun 2019 Krismayanti, Ni Kadek Putri Lia; Sueta, Made Agus Dwianthara; Mahayasa, I Made; Mulyawan, I Made
E-Jurnal Medika Udayana Vol 12 No 9 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i09.P04

Abstract

Colorectal cancer is a malignancy which that usually arises from the gland or epithelial cells of the large intestine. Based on data from GLOBOCAN 2018, colorectal cancer is the third most deadly and fourth most commonly cancer in the world. The aim of this study is to determine the profile of colorectal cancer patients at Sanglah General Hospital in 2019. This research is a descriptive study with the cross sectional method. Samples were selected from the population based on inclusion and exclusion criteria. The subjects of this study were 38 colorectal cancer patients at Sanglah General Hospital in 2019. Data were collected from medical records and it is analyzed using SPSS version 26. The result of this study showed that the profile of colorectal cancer patients at Sanglah General Hospital in 2019 greatly varied, which most occurred in the <60 years age group and dominated by male. Based on the occupation, most categories were miscellaneous (IRT and Retired) with a very normal BMI. All patients had no previous family history of colorectal cancer. The majority of the samples had a history of fiber consumption more than twice a day. Most of them did not consume alcohol with physical activity. The most common tumor’s location was in the rectum with the most histopathological findings of adenocarcinoma. Most tumor was in stage III and combination was the most common method for the management. This finding is beneficial because it provides insight into the description of colorectal cancer patients at Sanglah General Hospital in 2019. Further analytical research is indispensable to find the correlation between various variables. Keywords : Profile, Colorectal Cancer, Sanglah General Hospital
POLIMORFISME GEN COX-2 (rs20417) AND CHI3L1 (rs4950928) PADA PASIEN KANKER KOLOREKTAL DI BALI NI NYOMAN, AYU DEWI; Ekawati, Ni Putu; Mulyawan, I Made; Putra, I Wayan Ardyan Sudharta
E-Jurnal Medika Udayana Vol 12 No 12 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i12.P12

Abstract

Kondisi pro-inflamasi memiliki peran penting dalam perkembangan, invasi, dan metastasis kanker kolorektal (KKR). Beberapa penelitian mengungkapkan berbagai gen pro-inflamasi dalam karsinogenesis KKR, termasuk cyclooxygenase-2 (COX2) dan Chitinase-3-like protein 1 (CHI3L1). Penelitian ini bertujuan untuk mengidentifikasi polimorfisme gen COX-2 dan CHI3L1 pada pasien KKR di Bali. Data berasal dari 39 sampel pasien KKR, termasuk usia, jenis kelamin, diagnosis patologi, grade, dan lokasi tumor. DNA diekstraksi dari sampel FFPE. Genotipe SNP COX-2 (rs20417) dan CHI3L1 (rs4950928) diidentifikasi menggunakan real time quantitative PCR menggunakan primer spesifik (rhAmpTM SNP Genotyping System kit, IDT Technologies). Semua data dianalisis dengan SPSS versi 21. Hasil penelitian menunjukkan bahwa usia rata-rata adalah 59,82±11,35 tahun, lebih tinggi pada kelompok usia di atas 50 tahun sebanyak 31 sampel (79,5%). Sebagian besar sampel berjenis kelamin laki-laki sebanyak 24 sampel (61,5%) serta adenokarsinoma sebagai diagnosis patologis terbanyak sebanyak 32 sampel (82,1%). Selain itu, sebagian besar sampel menunjukkan low grade sebanyak 33 sampel (74,4%) dan lokasi tumor pada sisi kiri sebanyak 24 sampel (61,5%). Analisis hubungan SNP COX-2 (rs 20417) serta SNP CHI3L1 (rs4950928) dengan diagnosis patologi, grade dan lokasi menunjukkan nilai p>0,05. Secara keseluruhan, dapat disimpulkan bahwa kasus CRC lebih tinggi pada usia di atas 50 tahun, jenis kelamin laki-laki, low grade dan tumor sisi kiri. Dalam penelitian ini tidak ditemukan hubungan yang signifikan antara genotipe COX-2 dan CHI3L1 dengan diagnosis patologi, grade, dan lokasi tumor.
Identification of CDH4 Gene Copy Number Alteration and Its Association with Clinical Profile of Colorectal Cancer Patient Ayu Dewi, Ni Nyoman; Rompis, Andreliano Yosua; Mulyawan, I Made; Ekawati, Ni Putu; Sun, H Sunny
Indonesian Journal of Cancer Vol 18, No 3 (2024): September
Publisher : http://dharmais.co.id/

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33371/ijoc.v18i3.1135

Abstract

Background: The genes cadherin 4 (CDH4), Kirsten rat sarcoma viral oncogene homolog (KRAS), V-Raf murine sarcoma viral oncogene homolog B (BRAF), and microsatellite instability (MSI) each play a role in the development of colorectal cancer (CRC). CDH4 gene is located in chromosome 20q13.33 and its amplification or gain is the earliest mutational event found in the majority of CRC and colon polyps. This study aimed to identify copy number alteration in the CDH4 gene and its association with the clinical profile of CRC patients, including gender, age, tumor location and differentiation, frequency of BRAF and KRAS mutations, and MSI status. Methods: The DNA was extracted from 50 tumors and adjacent normal tissues based on the manufacturer’s instructions. Detection of MSI status was carried out by pentaplex polymerase chain reaction (PCR) and PCR products were size separated by capillary electrophoresis (CE) using an ABI 310 Genetic Analyzer. Both KRAS and BRAF mutations were identified by PCR and sequencing while CDH4 copy measurement was measured using TaqManTM Copy Number Assay. Results: Our findings showed 22 (44%) samples with no changes in the copy number of CDH4 gene. Interestingly, 21 (42%) cases had an amplification of the CDH4 gene or CDH4 gene gain, and seven (14%)  cases decreased in CDH4 gene expression or CDH4 gene loss. We found an association between changes in the CDH4 gene and gender (p=0.001). However, there was no association between changes in the CDH4 gene and age (p=0.979), tumor location (p=0.145) and differentiation (p=0.648), the frequency of BRAF (p=0.171) and KRAS mutations (p=0.184) and MSI status (p=0.923). Conclusions: Copy number alteration in CDH4 gene in CRC patients and this alteration is significantly associated with gender. Further studies with a larger number of samples are essential to confirm this result and to identify the cause of CDH4 copy number alteration and its biological significance.
Co-Authors Ayu Dewi, Ni Nyoman Krismayanti, Ni Kadek Putri Lia Mahayasa, I Made Ni Nyoman, Ayu Dewi Ni Putu Ekawati Putra, I Wayan Ardyan Sudharta Rompis, Andreliano Yosua Sueta, Made Agus Dwianthara Sun, H Sunny