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EFEKTIFITAS TRICHODERMA INDIGENUS SULAWESI TENGGARA SEBAGAI BIOFUNGISIDA TERHADAP Colletotrichum sp. SECARA IN- VITRO GUSNAWATY, HS; TAUFIK, MUHAMMAD; HERMAN, HERMAN
Jurnal Agroteknos Vol 4, No 1 (2014)
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This research aimed to determine the effectivity of Trichoderm isolates indigenous of Southeast Sulawesi as biofungicide to Colletotrichum sp in- vitro. This research was carried out in the Laboratory of Agrotechnology, Plant Pathology unit, Faculty of Agriculture, Halu Oleo University. Inhibition effectivity of 11 Trichoderma spp. isolates was done in double PDA media. This research used the complete randomized design (CRD). The observed variables were the inhibitory of Trichoderma spp. indigenous on the growth of Colletotrichum sp. Research result showed that the 11 isolates were effective as biofungicide against Colletotrichum sp. The data analysis showed that isolates DPA was the best isolate, inhibiting Colletotrichum sp. by 77.69%, otherwise LKP was isolate with the lowest inhibitory ability against Colletotrichum sp, by 65.07%. Inhibition mechanism of isolates DPA and DKT against Colletotrichum sp was by area competition and parasitism.Key words: Antagonist, Colletotrichum sp., Trichoderma Indigenous.

RESPON KETAHANAN BERBAGAI VARIETAS TOMAT TERHADAP PENYAKIT LAYU BAKTERI (Ralstonia solanacearum) Adriani, Adriani; Rahman, Abdul; Gusnawaty, HS; Khaeruni, Andi
Jurnal Agroteknos Vol 2, No 2 (2012)
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The study was aimed to know the resistance response of some tomato varities to bacterial wilt disease (Ralstonia solanacearum). The study was arranged in a completely randomized design (CRD) with six tomato variety treatmens, namely : Permata, Cosmonot, Idola, Gress, Ratna, and a local Muna variety. Each treatment had three replicates of six plants. The resistance response observed were : the latent period, disease incidence, disease severity, and plant height. The response was observed weekly until 7 weeks after inoculation. The results showed that Cosmonot variety was resistant to bacterial wilt disease, without any disease incedence occured and the disease severity was only 6,66%. Whereas the Local Muna variety as well as Permata, Gress, and Idola were susceptible to bacterial wilt disease. On the other hand, Ratna variety showed slight tolerant response. Keywords : bacterial wilt, Ralstonia solanacearum, resistance reaction, tomato variety

PENGARUH FUNGI MIKORIZA ARBUSKULA DAN NUTRISI ORGANIK TERHADAP PERTUMBUHAN TANAMAN CABAI MERAH BESAR (Capsicum annuum L.) Arma, Makmur Jaya; Risnawati, Risnawati; Gusnawaty, HS
Jurnal Agroteknos Vol 3, No 3 (2013)
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The research to study the effects of arbuscular mycorrhiza fungi (AMF) and organic nutrients to enhance the growth of chili has been conducted in Experimental Field, Faculty of Animal Husbandry and laboratory of Agrotechnology, Halu Oleo University, from June to November 2012. The research was based on the split-plot design with a randomized block design pattern (RAK) of two factors: Organic Nutrition as the main plot and AMF as subplot. Organic nutrients as the main plot consisted of three levels, namely: without organic nutrition (S0), 1 mL L-1 of water (S1) and 2 mL L -1 of water (S2); and AMF dose as subplot consisted of three levels, namely: without AMF (M0), 5 g plant-1 (M1) and 10 g plant-1 (M2). therefore, there were 9 combinations of treatments and each treatment combination was repeated three times to obtain 27 experimental units. Each variable was analyzed by analysis of variance, then followed by Duncan's Multiple Range Test (UJBD) at 95% confidence level. The results of research indicated that the best interaction of AMF and organic nutrient treatment was 10 g AMF plant-1 (M2) and 2 mL L-1 (S2) of organic nutrients. This treatment combination can improve growth on variables: leaf area, leaf area index and yield index of the chili plants. The best treatment for AMF independently was at 10 g plant-1 (M2) because it can promoted growth of plant height of the chili plants. The best treatment for organic matter independently was at 2 mL L-1(S2), because it can promoted growth of plant height of the chili plants. Keywords: FMA, organic nutrition, growth, chili

UJI EFEKTIVITAS BEBERAPA MEDIA UNTUK PERBANYAKAN AGENS HAYATI Gliocladium sp. Gusnawaty, HS; Taufik, Muhammad; Wahyudin, Edi
Jurnal Agroteknos Vol 3, No 2 (2013)
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This research was carried out in the Laboratory of Pests and Disease, Agricultural Faculty of Haluoleo University, from October to December 2012. This research was arranged in A Completely Randomized Design (CRD), with 7 treatments, namely: Gliocladium sp. in sago waste (a), Gliocladium sp. in cashew seed wastes (b), gliocladium sp. in saw dust waste (c), Gliocladium sp. in corn medium (d), Gliocladium sp. in bran medium (e), Gliocladium sp. in rice medium (f) and gliocladium sp. in rice husk waste (g). The research results showed that media used had different effectiveness. The most effective medium for Gliocladium sp. Propagation was rice bran medium, based on Gliocladium sp. growth (100%), and the number of Gliocladium sp. conidium ( 2,0 x105/g). Keywords: effectiveness, Gliocladium sp., medium, propagation

KAJI TINDAK PENGENDALIAN HAMA PENGGEREK BUAH KAKAO (Conopomorpha cramerella Snellen) DENGAN PESTISIDA NABATI Nuriadi, Nuriadi; Gusnawaty, HS
Jurnal Agroteknos Vol 3, No 1 (2013)
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This research aimed to know the effectivity of botanical pesticide in retarding cocoa pod borer. The study was conducted using completely randomized design, with 7 treatments and 3 replications. Total number of trial units was 21. The results of the study showed that the ingredient botanical pesticide that gave significant effect was ingredients III with interval 5 days/application which can limit attack of pod from 100 % to 24,78 % and intencity attack from 92,56 % to 11,25 %. Keywords : botanical pesticide, cocoa pod borer, intencity attack

EFEKTIFITAS INOKULASI ACTINOMYCETES DAN VAM DALAM MENGENDALIKAN PENYAKIT REBAH SEMAI (Sclerotium rolfsii ) PADA TIGA VARIETAS KEDELAI PADA MUSIM KEMARAU Gusnawaty, HS
Jurnal Agroteknos Vol 1, No 2 (2011)
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ABSTRACT The aim of the research was to know the effectiveness of actinomycetes and VAM inoculations in preventing the occurrence of infection of Sclerotium rolfsii on soybean at dry season. This research was conducted at the Phytophatology Laboratory, Mycology sub- Laboratory of the Plant Protection Faculty of Agriculture, University of Brawijaya, and in the trial plantation of Balai Benih Induk Palawija, Lawang, Malang subdistrict. This research used the Random Group Design (RGD) which consisted of two factors, namely (1) variety factor consisted of Ratai, Kaba and Burangrang; (2) inoculum factor, that were without actinomycetes and VAM inoculation, inoculation with actinomycetes, inoculation with VAM, combined inoculation of actinomycetes and VAM. The research result showed that the combined inoculation of actinomycetes and VAM could control the level of attack in three soybean varieties with the highest control effectiveness. Keywords: Actinomycetes, VAM, soybean, Sclerotium rolfsii

UJI POTENSI TRICHODERMA INDIGENOUS SULAWESI TENGGARA SEBAGAI BIOFUNGISIDA TERHADAP Phytophthora capsici SECARA IN-VITRO Gusnawaty, HS; Asniah, Asniah; Taufik, Muhammad; Faulika, Faulika
Jurnal Agroteknos Vol 3, No 3 (2013)
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This research was conducted in the Laboratory of Plant Pest and Disease, Department of Agrotecnologi, Faculty of Agriculture, Halu Oleo University Kendari, from May to August 2013. This study aimed to evaluate potential Trichoderma isolates indigeneous Southeast Sulawesi as biofungicide against Phytophthora capsici and Fusarium oxysporum in-vitro. The potential inhibitory test used multiple testing methods on PDA medium. The research design was a completely randomized design (CRD) consisting of 11 treatments (trichoderma isolates) with three replications. Variables measured were the inhibition of trichoderm a indigeneous on the growth of P. capsici and F. oxysporum. Results of the experiment showed that the trichoderma isolates were potential as biofungicide of P. capsici and F. oxysporum because they were able to inhibit the growth of pathogens in-vitro. All trichoderma isolates tested had the same potential as biofungicide against P. capsici, and isolate DKT, BPS, LKA, ASL, LTB, APS, DPA, LKO and DKP has the best potential as biofungicide against pathogenic F. oxysporum in-vitro. Keywords: F. oxysporum, inhibitory, indigenous of Southeast Sulawesi, P. capsici, trichoderma

KARAKTERISASI BIOKIMIAWI RIZOBAKTERI ASAL GULMA BERDAUN LEBAR YANG BERPOTENSI SEBAGAI DELETERIOUS RHIZOBACTERIA Asniah, Asniah; Rakian, Tresjia C.; Wangadi, Sri; Gusnawaty, HS
Jurnal Agroteknos Vol 3, No 3 (2013)
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The research aimed to know biochemical characters of rhizobacteria from broadleaf weed that are potential as deleterious rhizobacteria. The research was conducted at Agronomy Laboratory of Agriculture Faculty Halu Oleo University Kendari from January until March 2013. The results showed that 9 of 10 rhizobacteria isolates tested from broadleaf weeds rhizosphere had the ability to solubilize phosphate with different diameters. For nitrogen fixation ability, all isolates tested were potential but only isolates of ML-01 and KL-06 had high capability. All isolates had different ability to produce IAA, with isolates of KL-06 produced higher concentration of IAA (33,07 ppm) compared to other isolates. Isolates that had the ability to produce HCN were isolates BL-07, with filter paper change from yellow to dark brown, and BL-08 and BL-03 light brown color changes indicated to that the production of HCN was increased. Result of research showed that some isolates tested had biochemical character as deleterious rhizobacteria by the ability to solubilize phosphate, fix nitrogen, produce IAA and HCN. Keywords: biochemical characterization, rhizobacteria, broadleaf weeds, deleterious.

PENGGUNAAN Bacillus spp. SEBAGAI AGENS BIOKONTROL UNTUK MENGENDALIKAN PENYAKIT LAYU FUSARIUM PADA TANAMAN CABAI Khaeruni, Andi; Gusnawaty, HS
Jurnal Agroteknos Vol 2, No 3 (2012)
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The use of Bacillus spp. Bacteria as biocontrol agents is one alternative disease control for Fusarium wilt Fusarium oxysporum f.sp. Lycopersici on chilli plant . this study aimed to determine the effectiveness of Bacillus spp. As a biocontrol agent in various ways of application to control Fusarium wilt disease in pepper in in-vivo. Experiments were prepared using two factor factorial in a completely randomized design (CRD). Factor 1 (Applications) consisted of levels, namely: application of biocontrol agent Bacillus spp. M11 isolates (B1), application of biocontrol agents Bacillus spp. isolates F133 (B2), and application biocontrol agent Bacillus spp. mix. M11 isolates and isolates F133 (B3). The second factor (time of inoculation/application) consisted 3 levels, namely: inoculating the seed (S1), inoculation of Bacillus spp. simultaneously with pathogen inoculation (S2), inoculation of Bacillus spp. one week after pathogen inoculation (S3), to see the effect of the two isolates of Bacillus spp. that the latent period, the biocontrol agents Bacillus spp. mix isolates M11 and F133, was the best treatment with the longest latency period of the disease (16 hsi), the best plant growth by plant height reached 57,39 cm, and the lowest disease incidence and severity were 12,96 % and 11,11 %, respectively interaction between agent Bacillus spp. mix. Isolates M11 and F133 at the time of application on seed significantly affected the incidence of disease and plat height. Keyword: biocontrol agents, Bacillus spp., and Fusarium oxysporum f.sp. Lycopersici

EVALUASI KETAHANAN KULTIVAR SORGUM TERHADAP PENYAKIT LAYU FUSARIUM Asniah, Asniah; Gusnawaty, HS; Taufik, Muhammad
Jurnal Agroteknos Vol 3, No 2 (2013)
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This study aimed to evaluate the resistance of sorghum cultivars to wilt fusarium. This research was arranged in a randomized block design, with six treatments [brown sorghum, white sorghum and red sorghum with no inoculation fusarium, brown sorghum, white sorghum and red sorghum with inoculation fusarium] with 3 (three) replications. Data observed were plant height, leaf number, leaf area, and heaviest of grain weight for vegetative observation, while for disease character was disease incidence. The results showed that none of sorghum cultivars evaluated showed resistance to wilt fusarium infection. The tolerant response to wilt fusarium was observed on brown sorghum, whereas red and white sorghum were susceptible to wilt fusarium. The heaviest of grain weight was found in brown sorghum (27.86 g/penicle). The wilt fusarium caused disease incidences ranging from 33.33% (brown sorghum) to 50% (red sorghum). Keywords: cultivars, disease incidence, fusarium, sorghum, resistance

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