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All Journal ACTA VETERINARIA INDONESIANA Medical Journal of Indonesia Indonesian Journal of Biotechnology
Gunanegara, Rimonta Febby
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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The Bambara Groundnut’s Potential for Heart Histological Repair in Protein-Deficient Female Mice Puteri, Nabila Ramiza; Gunanegara, Rimonta Febby; Sunarti; Nuriliani, Ardaning
Acta VETERINARIA Indonesiana Vol. 11 No. 3 (2023): November 2023
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.11.3.234-243

Abstract

Defisiensi protein diketahui memberikan perubahan histologis yang signifikan pada jantung seperti hipertrofi, penebalan dinding ventrikel kiri, peningkatan deposisi matriks ekstraseluler dan diameter kardiomiosit, serta fibrosis interstisial. Kacang bambara (Vigna subterranea) dengan kandungan protein dan asam amino berpotensi untuk menanggulangi defisiensi protein dan memperbaiki perubahan struktur histologis pada jantung. Penelitian ini bertujuan untuk mempelajari pengaruh defisiensi protein 10% dan penambahan kacang bambara terhadap struktur histologis jantung mencit betina galur Swiss-Webster. Lima belas jantung dari 5 kelompok dikumpulkan untuk dipreparasi dengan metode parafin setebal 6 μm, dan diwarnai dengan Hematoxylin-Eosin dan Mallory Acid Fuchsin. Data dalam penelitian ini adalah parameter biometrik, lebar kardiomiosit, dimensi internal ventrikel kiri (LVID), ketebalan dinding posterior ventrikel kiri (LVPW), dan struktur histologis otot jantung. Data kuantitatif dianalisis dengan ANOVA satu arah dan post hoc dengan Duncan (p-value=0,05), sedangkan data kualitatif dianalisis menggunakan Kruskal Wallis (p<0,05). Tidak terdapat perbedaan yang signifikan (p>0,05) antara kelompok kontrol dan perlakuan pada parameter biometrik, LVID, LVPW, dan lebar kardiomiosit. Pada parameter struktur histologis otot jantung, kelompok defisiensi protein menunjukkan perubahan berupa lesi atrofi, hipertrofi, nekrosis, dan fibrosis yang lebih banyak dibandingkan dengan kelompok kacang bambara. Dengan demikian, penambahan kacang bambara berpotensi sebagai suplemen protein yang dapat memperbaiki struktur jantung pada kondisi defisiensi protein
Bambara groundnut ameliorates kidney histology in female mice with protein deficiency Firdiana, Vykra Aulia; Gunanegara, Rimonta Febby; Sunarti; Nuriliani, Ardaning
Medical Journal of Indonesia Vol. 32 No. 3 (2023): September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.oa.237030

Abstract

BACKGROUND Protein deficiency (PD) can lead to kidney damage. Consuming plant-based proteins may improve this condition. Bambara groundnut (Vigna subterranea)has an essential amino acid score of 80%, which is higher than other legumes; thus, it is potent in overcoming malnutrition. This study aimed to determine the effect of Bambara groundnut supplementation on kidney histology in adult female mice with PD. METHODS The study was conducted for 2 months in randomly selected female mice. These mice were grouped into the control, PD, and PD supplemented with Bambara groundnuts at 100, 200, and 300 g/kg of feed. 1 day after the last treatment, the kidneys of the mice were collected and processed histologically using the paraffin method (stained with hematoxylin and eosin and Masson’s trichrome). Parameters for observation included histopathological scoring (glomerular and interstitial space fibrosis and tubular damage), kidney histomorphometry, and organ index. Semi-quantitative data were analyzed using the Kruskal–Wallis test, while quantitative data were analyzed using one-way ANOVA (followed by Tukey’s test) and nested t-test. Statistical analysis was performed using SPSS software version 20 (IBM Corp., USA) (p≤0.05). RESULTS PD caused cell sloughing (moderate level) and dilatation (severe level) of the kidney tubules. It also reduced glomerular diameter and area by approximately 17.66% and 29%, respectively. PD and Bambara groundnut administration had no significant effects on the glomerular number, cortex and medulla thickness, distal and proximal tubule diameter, and kidney organ index (p>0.05). CONCLUSIONS Bambara groundnut (V. subterranea) administration prevented damage to the kidney’s histological structure of protein-deficient mice.
Anti‐inflammatory properties of conditioned medium from human Wharton’s jelly mesenchymal stem cells Azis, Rizal; Widowati, Wahyu; Gunanegara, Rimonta Febby; Kusuma, Hanna Sari Widya; Triharsiwi, Dwi Nur; Hadiprasetyo, Dhanar Septyawan; Gondokesumo, Marisca Evalina; Mars, Nicholas
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.107035

Abstract

Acute respiratory distress syndrome (ARDS) is a critical respiratory dysfunction triggered by intense in‐ flammation, microvascular damage, and increased epithelial and pulmonary vascular permeability. Human Wharton’s jelly mesenchymal stem cells (hWJMSCs) possess regenerative and anti‐inflammatory activities through the cytokines, chemokines, and growth factor secretion. The development of anti‐inflammatory agents derived from hWJMSCs has become one of the therapeutic solutions. Instead of direct cell use of hWJMSCs, their conditioned medium (CM) provides a cell‐free approach that delivers bioactive factors while minimizing the risks associated with stem cell transplantation. This study aims to measure the levels of vascular endothelial growth factor‐α (VEGF‐α), epidermal growth factor‐β (EGF‐β), interleukin‐10 (IL‐10), and hepatocyte growth factor (HGF) in CM‐hWJMSCs under non‐starvation and starvation conditions (24, 48 and 72 hours) using ELISA. The anti‐inflammatory potential of these factors was then analyzed through molecular docking with pro‐inflammatory cytokines. VEGF‐α, EGF‐β, IL‐10 and HGF levels were measured across all conditions. VEGF‐α ranged from 2590.37 to 3613.92 ng/mg protein; EGF‐β 347.01–504.43 ng/mg; IL‐10 302.59–729.28 pg/mg; and HGF 1747.20–2903.52 ng/mg. The molecular docking revealed strong binding between VEGF‐α, EGF‐β, IL‐10 and HGF with pro‐inflammatory cytokines, namely IL‐1β, IL‐6 and TNF‐α. VEGF‐α had the strongest bond with TNF‐α (–1162.3 kJ/mol), while EGF‐β formed the most hydrophobic and hydrogen interactions. The findings suggest that CM‐hWJMSCs, enriched with anti‐inflammatory and regenerative cytokines, may serve as a promising candidate for modulating the inflammatory pathways involved in ARDS pathogenesis. Longer starvation increased the secretion of VEGF‐α, EGF‐β, IL‐10 and HGF. These factors are known to promote angiogenesis, regulate immune responses, and protect against epithelial injury, thereby supporting the anti‐inflammatory and regenerative potential of hWJMSCs‐CM for ARDS therapy.
Co-Authors Ardaning Nuriliani Azis, Rizal Firdiana, Vykra Aulia Hadiprasetyo, Dhanar Septyawan Hanna Sari Widya Kusuma, Hanna Sari Widya Marisca Evalina Gondokesumo, Marisca Evalina Mars, Nicholas Puteri, Nabila Ramiza Sunarti Triharsiwi, Dwi Nur WAHYU WIDOWATI