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Comparative Studies of the Phytochemical Analysis, Mineral Content, Antibacterial Activity and Antioxidant Potential of Ethyl Acetate Extract of Alstonia Boonei Leaves and Stem Bark Uadia, Jeremiah Ogboma; Ogbeide, Osahon Kennedy
Walisongo Journal of Chemistry Vol 7, No 1 (2024): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v7i1.20667

Folkloric medicine has reported the effectiveness of Alstonia boonei stem bark and leaf extracts in treating and managing different ailments. Hence, this research is a comparative evaluation of the phytochemicals; antioxidant potential, mineral content, and antibacterial capacity of ethyl acetate extract of Alstonia boonei leaves and stem bark. Phytochemical analysis was determined using standard methods; mineral composition was determined using a flame photometer and an atomic absorption spectrophotometer; antibacterial capacity was analysed by agar-well diffusion method; and antioxidant potential was examined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. The phytochemicals detected were phenolics, eugenols, steroids, flavonoids, alkaloids, and reducing sugars in both extracts. Mineral content for sodium, iron, potassium, zinc, copper, magnesium and calcium was found to be 5.61 ± 1.33, 7.25 ± 1.00, 120.63 ± 1.39, 1.62 ± 0.30, 1.01 ± 0.01, 2.36 ± 0.10 and 15.61 ± 1.20 mg/kg respectively for the leaf extract, and 9.36 ± 0.35, 6.36 ± 0.55, 113.64 ± 0.45, 2.40 ± 0.32, 0.02 ± 0.00, 2.10 ± 0.50 and 28.30 ± 0.22 mg/kg respectively for the stem bark extract. The antioxidant potential at 250, 200, 150, 100, and 50 g/mL gave an IC50 of 2.89 ± 0.51, 3.90 ± 0.50, and 2.52 ± 0.22 μg/mL for leaf extract, stem bark extract and standard, respectively. The minimum inhibitory concentration (MIC) outcome was identical for both extracts except for P. aeruginosa. Both extracts' minimum bactericidal concentration (MBC) values were 12.5 to 50 mg/mL, except B. subtilis. Hence, this result provides valuable insight into the comparative usefulness of the plant in phytomedicine.

Phytochemical Investigation, Proximate Composition, Acute Toxicity, Anti-Inflammatory and Antinociceptive Activities of Extracts of Caesalpinia Pulcherrima Linn Flower Uadia, Jeremiah Ogboma; Nnamdi, Emeka Kingdom; Chigozie, Nnadozie; Ndubisi, Valerie Ifeanyi; Ogbeide, Osahon Kennedy
Walisongo Journal of Chemistry Vol 6, No 2 (2023): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v6i2.18175

The management and treatment of inflammation and pains have continued to gain increasing interest in recent times due to the challenge they pose to health. The study evaluated the phytoconstituents, proximate content, acute toxicity, antioxidant, anti-inflammatory, and antinociceptive properties of Caesalpinia pulcherrima flower extracts using different solvents. The bioactive chemical constituents, acute toxicity, and antioxidant property were investigated via standard methods while the anti-inflammatory and analgesic activities were determined using formalin-induced inflammation and acetic acid-induced writhing methods respectively. Phytochemicals determined were alkaloids, glycosides, saponins, tannins, phenolics, terpenoids, and steroids. The moisture, crude fibre, crude lipid and total ash content determined were 6.20 ± 0.01%, 15.00 ± 0.07%, 1.80 ± 0.03% and 7.00 ± 0.00% respectively. The n-hexane extract possessed the chief % inhibition (86.49 ± 0.30) and for ascorbic acid 88.29 ± 0.00 at 500 μg/mL extract from the antioxidant study. The acetone extract displayed the greatest significant anti-inflammatory potential at both 100 and 200 mg/kg dose at four hours, being the most effective and there exist a momentous reduction at p 0.05 in the writhes’ number in a manner depended on dose in the acetone and n-hexane extracts. At 5000 mg/kg, there was 100% mortality when the crude extracts were orally administered to the Swiss mice. This study ratifies that Caesalpinia pulcherrima flower extract is a potential spring of phytomedicine which could be applied for managing of inflammation, pain and oxidative stress-related infections but higher dosages could potentially be lethal.

Evaluation of the Influence of pH on the Release of Exchangeable Cations from a Mixed Clay Mineral Deposit for Replenishment of Soil Nutrients Irabor, Emmanuel Enoguan Ighodaro; Esanukpe, Linda; Imafidon, Monday Idiaghe; Ogbeide, Osahon Kennedy; Okunzuwa, Iyobosa Gift; Unuigbe, Charles Akhadelor; Omoruyi, Uwaila
Walisongo Journal of Chemistry Vol 6, No 1 (2023): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v6i1.16776

Soil nutrients consist of minerals such as Ca, Mg, K and Na ions among others which are essential to plant growth. Soils can become deficient in these nutrients on account of pollution, flooding and over cultivation. The soil condition can be ameliorated by addition of clay minerals which are rich in aforementioned cations. The mineralogy of the clay mineral was investigated using X-ray Diffractometer (XRD) and the effect of pH variation on the release of exchangeable cations of the clay mineral deposit were investigated by varying the pH of the solution in which the clay mineral was immersed whereas the amount of the cations released were determined using Atomic Absorption Spectrophotometer(AAS) and Flame Emission Spectrophotometer. Three clay minerals clinochlore, kaolinite and illite were detected. The quantity of cations released from the clay mineral varied with pH; and the pH at which the maximum quantity of the cations were released were pH4 for K+ ions(4.63±0.38cmol/kg), pH7 Ca2+ (12.09±0.96 cmol/kg) and Mg2+(3.48±0.08 cmol/kg) ions and pH8 for Na+(7.81±0.58 cmol/kg) ions respectively for site 1. This trend was observed in the three sites that were studied. This study has revealed that different pH conditions are required if this mixed clay mineral is to be employed in the remediation of nutrient deficiency in any soil in respect of a particular mineral.

Qualitative and Quantitative Phytochemical Constituents, Antioxidant Activities, and Antimicrobial Studies of Methanol Extract of Morinda citrifolia Stem Ogbeide, Osahon Kennedy; Aghedo, Oscar Notoriuwa; Akhidenor, Faith Isimemen; Dibie, Edward Nduka; Iyasele, Julius Uche
Sciences of Phytochemistry Volume 4 Issue 2
Publisher : ETFLIN Publishing House

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/sciphy0402368

This study evaluated the qualitative and quantitative phytochemical constituents, antioxidant potential, and antimicrobial properties of Morinda citrifolia stem. The stem extract was prepared using the maceration method with 99.8% methanol for three days at room temperature. Phytochemical and proximate compositions were analyzed using standard methods, while selected minerals were determined by atomic absorption spectrophotometry. Antioxidant activity was assessed using the DPPH free radical scavenging assay, and antimicrobial activity was examined by the agar-well diffusion method. Phytochemical screening revealed the presence of phenolics, glycosides, steroids, terpenoids, alkaloids, tannins, flavonoids, and eugenols. Proximate analysis showed moisture, lipid, protein, ash, fiber, and carbohydrate contents of 18.99%, 9.12%, 4.72%, 6.40%, 37.90%, and 22.87%, respectively. The concentrations of Na, Mg, K, Ca, Fe, Cu, and Zn were 7.51 ± 1.60, 126.67 ± 9.43, 91.12 ± 16.12, 7.59 ± 0.63, 3.50 ± 0.65, 0.02 ± 0.003, and 0.26 ± 0.086 mg/kg, respectively. The extract exhibited strong antioxidant activity with an IC₅₀ of 49.48 µg/mL, comparable to ascorbic acid (46.56 µg/mL). Antimicrobial testing showed inhibition of Staphylococcus aureus growth, with minimum inhibitory and bactericidal concentrations of 500 mg/mL and 50–200 mg/mL, respectively. These results demonstrate that M. citrifolia stem methanol extract possesses significant antioxidant and antimicrobial activities, supporting its potential as a natural therapeutic and its traditional medicinal applications.

Comparative Studies of the Phytochemical Analysis, Mineral Content, Antibacterial Activity and Antioxidant Potential of Ethyl Acetate Extract of Alstonia Boonei Leaves and Stem Bark Uadia, Jeremiah Ogboma; Ogbeide, Osahon Kennedy
Walisongo Journal of Chemistry Vol. 7 No. 1 (2024): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology UIN Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v7i1.20667

Folkloric medicine has reported the effectiveness of Alstonia boonei stem bark and leaf extracts in treating and managing different ailments. Hence, this research is a comparative evaluation of the phytochemicals; antioxidant potential, mineral content, and antibacterial capacity of ethyl acetate extract of Alstonia boonei leaves and stem bark. Phytochemical analysis was determined using standard methods; mineral composition was determined using a flame photometer and an atomic absorption spectrophotometer; antibacterial capacity was analysed by agar-well diffusion method; and antioxidant potential was examined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. The phytochemicals detected were phenolics, eugenols, steroids, flavonoids, alkaloids, and reducing sugars in both extracts. Mineral content for sodium, iron, potassium, zinc, copper, magnesium and calcium was found to be 5.61 ± 1.33, 7.25 ± 1.00, 120.63 ± 1.39, 1.62 ± 0.30, 1.01 ± 0.01, 2.36 ± 0.10 and 15.61 ± 1.20 mg/kg respectively for the leaf extract, and 9.36 ± 0.35, 6.36 ± 0.55, 113.64 ± 0.45, 2.40 ± 0.32, 0.02 ± 0.00, 2.10 ± 0.50 and 28.30 ± 0.22 mg/kg respectively for the stem bark extract. The antioxidant potential at 250, 200, 150, 100, and 50 g/mL gave an IC50 of 2.89 ± 0.51, 3.90 ± 0.50, and 2.52 ± 0.22 μg/mL for leaf extract, stem bark extract and standard, respectively. The minimum inhibitory concentration (MIC) outcome was identical for both extracts except for P. aeruginosa. Both extracts' minimum bactericidal concentration (MBC) values were 12.5 to 50 mg/mL, except B. subtilis. Hence, this result provides valuable insight into the comparative usefulness of the plant in phytomedicine.

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