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Arianto, Anayanti
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Journal : International Journal Of Science, Technology

 1 
Phytochemical Screening And Antibacterial Activity Of The Ethanol Extract Of Putri Malu (Mimosa Pudica Linn.) Leaves Combined With Klanceng Honey Against Staphylococcus Aureus Bacteria Dalimunte, Khairunnisa; Arianto, Anayanti; Salim, Emil
International Journal of Science, Technology & Management Vol. 4 No. 6 (2023): November 2023
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v4i6.965

Abstract

Mimosa pudica Linn. Leave and honey have been known to have antibacterial activity. The combination of the two is very beneficial as an antibacterial because the hydrogen peroxide content in honey and the flavonoid metabolite compounds in Putri Malu provide a synergistic effect as an antibacterial. The aim of this research was to determine the activity of the ethanol extract of Putri Malu leaves combined with Klanceng honey using the disc diffusion method against Staphylococcus aureus bacteria. The results showed that the resulting extract was thick and blackish-green in colour, with a yield of 25.6%. The results of the phytochemical screening test showed that Putri Malu extract contains alkaloids, flavonoids, glycosides, saponins, and tannins. Characterization results showed that the water content of the extract was 7.28 ± 0.05, water-soluble essence content was 16.03 ± 0.08, ethanol-soluble essence content was 19.65 ± 0.01, total ash content was 4.70 ± 0.00, and essence content acid soluble was 0.17±0.02. Antibacterial testing showed that the best ratio of Putri Malu leaf extract to honey to inhibit Staphylococcus aureus was 9:1, with an inhibitory value of 13.60 ± 0.40 mm. In conclusion, the combination of Putri Malu leaf extract with honey strongly inhibits the growth of Staphylococcus aureus bacteria.
Antibacterial Activity and Determination of Total Phenol and Flavonoid of Carica papaya L. Ethanol Extract Karo-Karo, Sry Ulina; Arianto, Anayanti; Salim, Emil
International Journal of Science, Technology & Management Vol. 4 No. 1 (2023): January 2023
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v4i1.738

Abstract

Infectious diseases are Indonesia's most common. Antibiotics are used to treat infections. Medicine needs new antibiotics due to antibiotic resistance. Antibiotics from plants are possible. Papaya leaf carpain alkaloids may be antibacterial. This study determined papaya leaf compound content, flavonoids' total phenol content, and the optimal concentration of papaya leaf ethanol extract that inhibits Staphylococcus aureus and Pseudomonas aeruginosa. The first stages of the research were the maceration of papaya leaf extract with 96% ethanol solvent, the phytochemical screening test (alkaloid, flavonoid, saponin, tannin, and steroid and triterpenoid tests), total phenol and total flavonoids tests, and the paper disc antibacterial activity test. Alkaloids, flavonoids, glycosides, tannins, steroids, and saponins were found in papaya leaf ethanol extract. Papaya leaf ethanol extract has 59.05 mgQE/g flavonoids and 56.80 GAE/g phenols. The antibacterial activity test of papaya leaf ethanol extract measured the diameter of the inhibitory zone on Staphylococcus aureus and Pseudomonas aeruginosa. Alkaloids, flavonoids, glycosides, tannins, steroids, and saponins were found in papaya leaf ethanol extract. Papaya leaf ethanol extract has 59.05 mgQE/g flavonoids and 56.80 GAE/g phenols. Papaya leaf ethanol extract had the best inhibition zone diameter for Staphylococcus aureus at 150 mg/mL (12.85 mm) and Pseudomonas aeruginosa at 150 mg/mL (13.10 mm).
Phytochemical Screening of Cinnamon Bark (Cinnamomum burmanii) (C. Ness & T. Ness) C. Ness ex Blume Ethanol Extract and Antioxidant Activity Test with DPPH (2,2-diphenyl-1-picrylhydrazyl) Method. Sirait, Tri Susanti; Arianto, Anayanti; Dalimunthe, Aminah
International Journal of Science, Technology & Management Vol. 4 No. 1 (2023): January 2023
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v4i1.739

Abstract

Cinnamon (Cinnamomum burmanii) is one of the spices that can be used as cosmetic preparations in the pharmaceutical industry because it contains potent antioxidants. Examples of cosmetic products containing cinnamon are body scrubs, body showers, and moisturizers. This study aimed to determine the antioxidant activity of cinnamon extract using the DPPH method. Cinnamon was extracted using the maceration technique with 96% ethanol solvent, and phytochemical screening was carried out. The results of phytochemical screening from cinnamon bark extract were alkaloids, saponins, tannins, triterpenoids, and flavonoids. Antioxidant activity obtained an IC50 value of 25.35 ppm. Based on these results, the cinnamon extract has a very strong antioxidant activity value.
Antibacterial Activity Of Lime Peel (Citrus Aurantifolia (Christm.) Swingle) Ethanolic Extract Against Skin Infection Bacteria (Propionibacterium Acnes And Staphylococcus Epidermidis) Sormin, Novia; Arianto, Anayanti; Pandapotan, M.
International Journal of Science, Technology & Management Vol. 4 No. 4 (2023): July 2023
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v4i4.841

Abstract

The skin is the outermost part of the body that serves as a protective body. In addition to protecting the skin of the face, it also acts as a beautifier. The main problem with facial skin is inflammation of the skin, which can cause acne. Bacteria like Propionibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa can cause acne on the face. Prolonged use of antibiotics as therapy against acne will cause a new problem, namely resistance to antibiotics. Lime peel has many uses, especially as an antibacterial, which makes it a good candidate for use as an antibacterial agent in acne treatment. The goal of this study was to find out if the etTMhanolic extract of lime peel could kill bacteria like Propionibacterium acnes and Staphylococcus epidermidis. The research method used the Kirby-Bauer method and the Streaking methods to analyze the minimum inhibitory concentration and minimum bactericidal concentration. The results showed MIC at a concentration of 1 mg/mL for each bacterium, while MBC at a concentration of 40 mg/mL in Propionibacterium acnes and 50 mg/mL in Staphylococcus epidermidis. The conclusion is that the extract of lime peel in ethanol can kill bacteria.
Co-Authors . S, Aminah Aminah Dalimunthe Dalimunte, Khairunnisa Emil Salim Hakim Bangun* Karo-Karo, Sry Ulina Pandapotan, M. Sormin, Novia Tobing, Johnson L. Tri Susanti Sirait