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All Journal Jurnal Bahan Alam Terbarukan Jurnal Bioteknologi & Biosains Indonesia
Amelia, Vika
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Biochemistry, Genetics & Molecular Biology Materials Science & Nanotechnology

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Process of Bacterial Cellulose Production from Tofu Wastewater Without Pretreatment Using Acetobacter xylinum Margono, Margono; Isnaeni, Nur; Amelia, Vika; Shohih, Esa Nur
Jurnal Bahan Alam Terbarukan Vol 12, No 2 (2023): December 2023 [Nationally Accredited Sinta 2]
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbat.v12i2.47510

Abstract

Bacterial cellulose (BC) is an alternative cellulose source that warrants more investigation due to its limited efficiency and high production costs. This research aims to investigate the generation of bacterial cellulose from tofu wastewater using Acetobacter xylinum without pretreatment. The experiment was run on two media: tofu wastewater with and without additional sugar. The fermentation was run in a dark cabinet using a static batch method in numerous fermenter trays, each with a working volume of 1000 mL. Each experiment used 900 mL of tofu wastewater medium plus an extra 100 mL inoculum (equal to 10% v/v), which was then cultured at room temperature and harvested on days 6, 12, 18, 24, and 30. The weight of nata de soya, BC, and residual sugar were all measured from the samples. The results of the experiments revealed that the best incubation time was 18 days. The fermentation employing tofu wastewater medium with added sugar yielded nata de soya of 227.3 g/L and BC of 32.2 g/L, while the medium without added sugar yielded 103.9 g/L and BC of 9.3 g/L. The medium with added sugar yielded higher BC productivity, 1.79 g/L.day, compared to 0.57 g/L.day in the medium without added sugar. On the other hand, the BC results per sugar consumption were 0.62 g BC/g sugar and 0.36 g BC/g sugar, respectively, for the medium with and without added sugar.
ANALYSIS OF INTERLEUKIN-10 LEVELS IN MESENCHYMAL STEM CELL SECRETOME CREAM WITH ELISA METHOD Amelia, Vika; Marlina; Sudji, Ikhwan Resmala
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2610

Abstract

Mesenchymal Stem Cells (MSC) are cells that are able to differentiate into other cells and have immunomodulatory properties that are used to treat inflammatory diseases. MSCs have the ability to repair damaged tissue by regenerating damaged tissue. MSCs produce a secretome, where the MSC secretome has various cytokines, chemokines, growth factors, anti-inflammatory factors and proteins that are produced in molecular form. One of the molecules secreted by the secretome is Interleukin-10.IL-10 is an anti-inflammatory cytokine which functions to inhibit the production of several other types of cytokines such as TNF, IL-1, chemokine and IL-12). Apart from that, IL-10 is able to inhibit the function of macrophages in assisting T cell activity. One of the products from Secretome MSC is a cream preparation. To find out whether the MSC cream contains IL-10 protein, Interleukin-10 levels were analyzed in the MSC secretome cream with 3 different MSC cream concentration formulations (2%, 3% and 5%). Analysis of IL-10 levels was carried out using ELISA to detect IL-10 antigen. The results of the examination of 9 samples showed that all samples were positive for IL-10 with a concentration of 2% dilution 1:2 showing the best results with an Optical Density (OD) of 0.069 and followed by a concentration of 5% dilution 1:2 showing an OD value of 0.065.
Co-Authors Esa Nur Shohih Ikhwan Resmala Sudji ISNAENI, NUR Margono Margono Marlina