This research aims to determine aspects of cetol fish reproductive biology including sexuality, sex ratio, GSI, gonadal maturity (TKG), and fecundity, in order to obtain information as a basis for cultivation. Samples were taken in 6 waters in the Magelang Regency area, during August at 1 week intervals. The data obtained is presented in the form of tables and figures, analyzed descriptively. Water quality is classified as good, temperature range 22.5-29.8 °C, pH 6.09-6.80, and DO 7.0-15.7 mg/L. Cetol fish have sexual dimorphism and dichromatism. The male:female sex ratio is 1.1:1. Male and female fish in TKG I-VI, with a greater percentage of males and females in TKG III-VI than in TKG I-II. At a length of 1.6-2.0 cm, males have been found in TKG III-V and females in TKG III-IV, and at a length of 2.1-2.5 cm, males and females have been found in TKG III-VI. The GSI of male fish reaches 8.22% (1.2-2.5 cm) and for females it reaches 28.09% (1.1-3.8 cm). Fecundity ranges from 2-36 eggs (1.1-3.8 cm and 0.07-0.57 g). The conclusion of this research is that the sex ratio is unequal, male and female cetol fish first mature gonads at a length of 1.6-2.0 cm, and females can give birth to young at a length of 2.1-2.5 cm. August is the spawning season for cetol fish marked by high GSI and TKG advanced. Cetol fish are viviparous so fecundity is low.