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All Journal El-Hayah : Jurnal Biologi Biosaintifika: Journal of Biology & Biology Education Biosaintifika: Journal of Biology & Biology Education
Altuhaish, Adeel Abdulkarim Fadhl
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology

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Verification of Maman (Cleome gynandra (L.) Briq.) from Riau Based on matK and trnL-trnL-trnF Intergenic Spacer Roslim, Dewi Indriyani; Herman, Herman; Putri, Shalsadila Rahmadani; Furqoni, Aries Tri; Budiono, Deanne Yoshe Fidela; Altuhaish, Adeel Abdulkarim Fadhl; Lestari, Wahyu
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.46937

Abstract

Maman is a traditional plant frequently used as food by people in Melayu Rokan of Riau Province. Morphological detection indicates that the scientific name of maman is Cleome gynandra L.  The objective of this study is to confirm the taxonomic status of maman from Riau basedon matK and trnL-trnL-trnF intergenic spacer (IGS) sequences. Methods utilized in this study included sampling, DNA extraction, PCR, electrophoresis, sequencing, and data analysis. The fresh leaves were picked up from Rokan Hulu Regency, Riau Province. In this study, the matK and trnL-trnL-trnF IGS sequences of maman with a length of 754 bp and 938 bp, respectively, had been obtained. The BLASTn analysis based on both sequences showed that maman had 100% similarity to Gynandropsis gynandra. Cleome gynandra was synonymous with G. gynandra. There were 49 nucleotide variations, 16 critical nucleotides, and there were no indels in matK. Meanwhile, in trnL-trnL-trnF IGS there were 181 nucleotide variations, 13 critical nucleotides, and 61 indels. In conclusion, this study succeeded in confirming the taxonomic status of maman from Riau as C. gynandra syn. G. gynandra. The result enriched the abundance of DNA barcode sequences of this plant in GenBank which is useful for the molecular identification.
Inorganic Fertilizer Efficiency Using Liquid Organic Fertilizer on Peanut Growth and Production (Arachis hypogaea L.) Dasumiati, Dasumiati; Nurrahmah, Annisa; Khairiah, Ardian; Junaidi, Junaidi; Altuhaish, Adeel Abdulkarim Fadhl
El-Hayah:Jurnal Biologi Vol 10, No 1 (2024): EL-HAYAH (VOL 10, NO 1 September 2024)
Publisher : Program Study of Biology, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v10i1.28962

Abstract

Fertilization is important in increasing crop production. The use of liquid organic fertilizer (LOF) in addition to inorganic fertilizer in crop cultivation is expected to reduce the use of inorganic fertilizer and increase growth and production. The research aimed to determine the effect of LOF on peanut growth and production and to obtain the right dose to reduce the use of inorganic fertilizer doses. The research used a factorial Randomized Group Design (RGD) with two factors. The first factor was LOF dose (0, 3, 5, and 7 ml/L/Plot) and the second factor was inorganic fertilizer dose (0, 25, 50, and 100% of the recommended dose). Data analysis using ANOVA and DMRT. The results showed that the dose of LOF had a very significant effect (P0.01) on plant height, number of leaves, leaf area, number of filled pods, and total number of pods. The best dose in improving all these parameters was LOF 3 ml/L/Plot. The combination treatment of LOF and inorganic fertilizer had a very significant effect (P0.01) on the number of filled pods, but not significant effect (P0.05) on other parameters. The best combination of LOF dose and inorganic fertilizer was C1A1 (3 ml/L/Plot LOF + 25% Inorganic) which produced almost the same number of filled pods as C0A2 (0 ml/L/Plot LOF + 50% Inorganic), and higher than other treatments. The C1A3 treatment also produced higher fresh pod weight and dry pod weight than the other treatments. The application of LOF 3 ml/L/Plot + 25% inorganic fertilizer from the recommended dose can increase peanut production, and can reduce the use of inorganic fertilizer by 75%.
Molecular Characteristics of Lopang (Gymnopetalum cochinchinense) Originating from Riau Based on matK and trnL-trnF Intergenic Spacer Herman, Herman; Akmal, Fidia; Nurbaiti, Nurbaiti; Siahaan, Citra Winarni; Lestari, Wahyu; Adiwirman, Adiwirman; Altuhaish, Adeel Abdulkarim Fadhl; Roslim, Dewi Indriyani
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.24522

Abstract

Lopang (Gymnopetalum cochinchinense) is a herbaceous plant traditionally consumed as a vegetable by communities in Riau Province, Indonesia. However, DNA barcode sequence data for this species remain limited. These findings underscore the importance of analyzing DNA barcode sequences of matK and trnL-trnF intergenic spacer (IGS) lopang from Riau. This study highlights the analysis of DNA barcode sequences matK and trnl-trnf intergenic spacer (IGS) in lopang from Riau province. This study applies sampling, DNA extraction, Polymerase Chain Reaction (PCR), electrophoresis, sequencing, and bioinformatics data analysis using BioEdit 7, BLASTn (Basic Local Alignment Search Tool) to find sequence similarities with the GenBank database, Management and analyzed DNA sequences with MESQUITE, Multiple alignments using ClustalW and MEGA11 to create dendrograms. Fresh leaves were collected from Rokan Hulu Regency in Riau Province. The results showed that matK and trnL-trnF IGS DNA sequences of lopang measured 752 bp and 410 bp, respectively. BLASTn analysis revealed that lopang has 99.73% similarity with G. chinense based on the matK sequence and 99.76% similarity based on the trnL-trnF IGS sequence. The analysis revealed a variation of 13 nucleotides, 1 critical nucleotide and no indels in the matK sequence, while in the trnL-trnF IGS sequence there were 19 nucleotide variations, 1 critical nucleotides and 5 indels. As a final point, lopang from Riau is closely related to G. chinense. The findings of this research contribute to the molecular identification of this species and benefit science, such as providing an understanding of plant evolution, species identification, genetic analysis, and the development of molecular markers in the Cucurbitaceae family.
Co-Authors Adiwirman Adiwirman, Adiwirman Akmal, Fidia Aldri Frinaldi Budiono, Deanne Yoshe Fidela Dasumiati Dasumiati Dewi Indriyani Roslim Furqoni, Aries Tri Herman Junaidi Junaidi Khairiah, Ardian Nurrahmah, Annisa Nurwijayanti Putri, Shalsadila Rahmadani Siahaan, Citra Winarni Wahyu Lestari