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ISOLASI DAN AMPLIFIKASI DNA KELADI TIKUS (Thyponium flagelliform) UNTUK IDENTIFIKASI KERAGAMAN GENETIK Hikmatyar, Mohamad Fazri; Royani, Juwartina Ida; ., Dasumiati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 2, No 2 (2015): December 2015
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Keladi tikusÂ (Typhonium flagelliforme) is one of considerable potential medicinal plants, especially as anticancer herbal medicine. In Indonesia, this plant grows throughout the island of Java, in part of Kalimantan, Sumatra and Papua. The development of Keladi tikus plants to provide raw material to meet public demand is constrained with the quality of the plants that is not standardized yet. DNA marker technique has been widely used for identification of standardization and diversity of varieties. The aims of this research were to isolate DNA from 17 accessions of Keladi tikus from various regions in Indonesia and to amplify the DNA using ISSRÂ primers. The results obtained were 17 accessions of Keladi tikus that had been isolated using the modified CTAB method. Amplifications were done by using SBLT2 and SBLT8 primers that facilitated the appearance of the polymorphism bands on the 17 accessions of Keladi tikus. Thus, SBLT2 and SBLT8 primers can be used to identify genetic variations of Keladi Tikus.Keywords: Typonium flagelliforme, Keladi tikus, ISSR, medicinal plant, amplificationÂ ABSTRAKKeladi tikusÂ (Typonium flagelliforme) merupakan salah satu tanaman obatÂ yang cukup potensial khususnya sebagai obat herbal antikanker. Tanaman ini di Indonesia tersebar di sepanjang Pulau Jawa, sebagian Kalimantan, Sumatera dan Papua. Pengembangan tanaman keladi tikus untuk memenuhi bahan baku kebutuhan masyarakat saat ini terkendala pada mutu tanaman tersebut yang belum terstandar. Teknik penanda DNA telah banyak digunakan untuk standarisasi dan identifikasi keragaman varietas. Tujuan dari penelitian ini adalah untuk mengisolasi DNA dari 17 aksesi Keladi tikus dari berbagai daerah di Indonesia dan mengamplifikasi DNA tersebut dengan primer ISSR. Hasil penelitian menunjukkan bahwa 17 aksesi keladi tikus telah dapat diisolasi dengan menggunakan metode CTAB yang dimodifikasi. Amplifikasi dilakukan dengan primer SBLT2 dan SBLT8 yang mampu memunculkan pita-pita polimorfisme pada ke 17 aksesi Keladi tikus. Primer SBLT2 dan SBLT8 dapat digunakan untuk identifikasi variasi genetic Keladi tikus.Kata Kunci: Keladi tikus, Typonium flagelliforme, ISSR, tanaman obat, amplifikasi

Tetumbuhan Yang Dimanfaatkan Oleh Penduduk Desa Neglasari Kabupaten Sukabumi Jawa Barat Sebagai Obat Yatias, Ellyf Aulana; Priyanti, Priyanti; Dasumiati, Dasumiati
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 14 No 1 (2019): Bioprospek: Jurnal Ilmiah Biologi: Volume 14 Number 1 2019
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Berkembangnya industri farmasi yang memproduksi obat-obat sintetis menjadikan penduduk di Desa Neglasari Kabupaten Sukabumi Jawa Barat mulai meninggalkan penggunaan tumbuhan sebagai obat. Oleh karena itu perlu adanya kajian tentang tetumbuhan obat yang digunakan penduduk desa ini. Inventarisasi tumbuhan obat dilakukan di dusun Baros I, Baros II, Cijureuy, dan Cibodas. Metode wawancara semi terstruktur dilakukan terhadap paraji dan penduduk yang memiliki pengetahuan tentang tumbuhan obat. Berdasarkan hasil penelitian, tetumbuhan obat yang berhasil diidentifikasi berjumlah 64 jenis yang termasuk anggota Zingiberaceae sebesar 16.21%, sedangkan sebanyak 2.7-10.81% termasuk 36 suku lainnya. Nilai kegunaan (use value) tertinggi (8.48), yaitu Strobilanthes crispus, sedangkan nilai kegunaan terendah (5.97) adalah Ricinus communis. Daun adalah bagian yang paling banyak (32.35%) digunakan sebagai bahan baku obat, sedangkan umbi, rebung, ranting, dan kulit batang digunakan sebagai obat hanya sebesar 0.98%. Kelompok penyakit tidak menular paling banyak (46.59%) diobati dengan tumbuhan dibandingkan kelompok penyakit kronik (14.17%). Perebusan adalah cara pengolahan terbanyak (37.59%) untuk memperoleh khasiatnya sebagai obat dibandingkan dengan cara pengolesan, penetesan, dan peletakan pada bagian yang sakit (0.75%). Pemanfaatan tumbuhan obat oleh penduduk Desa Neglasari Kabupaten Sukabumi Jawa Barat bervariasi sehingga perlu diupayakan ketersediaan bahan baku tumbuhan obat di habitat alaminya dan pengetahuan lokal masyarakatnya pun dapat diketahui secara luas.

Angsana (Pterocarpus indicus) sebagai Bioindikator untuk Polusi di Sekitar Terminal Lebak Bulus Waryanti, Waryanti; Sugoro, Irawan; Dasumiati, Dasumiati
Al-Kauniyah: Jurnal Biologi Vol 8, No 1 (2015): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Jakarta as one of the biggest city in Indonesia, it own more than 6.506.244 units of motor vehicles. The combustion of fossil fuels in motor vehicles can cause air pollution. Station is represented as one of heavy pollution location. The observation has be done by abservate the amount of vehicles, stomatal characteristic, and weight of dust on Angsana leaves, which growth around Lebak Bulus station. As the result, there is relation between weight of dust on leaf and stomatal characteristic, with r value = 1. The level weighat of dust on leaf has effect to stomata conditions, such as surface of stomata become smaller and shape of stomata become irregular, but amount and size of stomata didnâ€™t influenced. In some case, leaf can be identifid by visible symptoms of injury such as chlorotic at the leaf veins, which caused by SO2 or black or brown flecks at the leaf veins, which caused by NOx.

ISOLASI DAN AMPLIFIKASI DNA KELADI TIKUS (Thyponium flagelliform) UNTUK IDENTIFIKASI KERAGAMAN GENETIK Hikmatyar, Mohamad Fazri; Royani, Juwartina Ida; ., Dasumiati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 2 (2015): December 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Keladi tikusÃ‚Â (Typhonium flagelliforme) is one of considerable potential medicinal plants, especially as anticancer herbal medicine. In Indonesia, this plant grows throughout the island of Java, in part of Kalimantan, Sumatra and Papua. The development of Keladi tikus plants to provide raw material to meet public demand is constrained with the quality of the plants that is not standardized yet. DNA marker technique has been widely used for identification of standardization and diversity of varieties. The aims of this research were to isolate DNA from 17 accessions of Keladi tikus from various regions in Indonesia and to amplify the DNA using ISSRÃ‚Â primers. The results obtained were 17 accessions of Keladi tikus that had been isolated using the modified CTAB method. Amplifications were done by using SBLT2 and SBLT8 primers that facilitated the appearance of the polymorphism bands on the 17 accessions of Keladi tikus. Thus, SBLT2 and SBLT8 primers can be used to identify genetic variations of Keladi Tikus.Keywords: Typonium flagelliforme, Keladi tikus, ISSR, medicinal plant, amplificationÃ‚Â ABSTRAKKeladi tikusÃ‚Â (Typonium flagelliforme) merupakan salah satu tanaman obatÃ‚Â yang cukup potensial khususnya sebagai obat herbal antikanker. Tanaman ini di Indonesia tersebar di sepanjang Pulau Jawa, sebagian Kalimantan, Sumatera dan Papua. Pengembangan tanaman keladi tikus untuk memenuhi bahan baku kebutuhan masyarakat saat ini terkendala pada mutu tanaman tersebut yang belum terstandar. Teknik penanda DNA telah banyak digunakan untuk standarisasi dan identifikasi keragaman varietas. Tujuan dari penelitian ini adalah untuk mengisolasi DNA dari 17 aksesi Keladi tikus dari berbagai daerah di Indonesia dan mengamplifikasi DNA tersebut dengan primer ISSR. Hasil penelitian menunjukkan bahwa 17 aksesi keladi tikus telah dapat diisolasi dengan menggunakan metode CTAB yang dimodifikasi. Amplifikasi dilakukan dengan primer SBLT2 dan SBLT8 yang mampu memunculkan pita-pita polimorfisme pada ke 17 aksesi Keladi tikus. Primer SBLT2 dan SBLT8 dapat digunakan untuk identifikasi variasi genetic Keladi tikus.Kata Kunci: Keladi tikus, Typonium flagelliforme, ISSR, tanaman obat, amplifikasi

Keragaman dan Kepadatan Populasi Burung di Kawasan Hijau Cibinong Science Center (CSC) LIPI, Jawa Barat Maulidya, Anggi Lelia; Dasumiati, Dasumiati; Widodo, Wahyu
Al-Kauniyah: Jurnal Biologi Vol 14, No 2 (2021): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v14i2.19942

AbstrakBurung merupakan satwa liar yang berperan penting dalam penyusun rantai makanan, membantu penyerbukan, mengendalikan populasi serangga hama, dan agen penyebar biji yang bermanfaat untuk meregenerasi hutan secara alami. Peran penting terhadap lingkungan yang menjadikan burung sebagai indikator kualitas lingkungan. Cibinong Science Center (CSC) LIPI merupakan kawasan yang seiring berjalannya waktu mengalami perubahan lanskap karena terdapat bangunan-bangunan baru yang menyebabkan area terbuka hijau menjadi berkurang. Hal tersebut menyebabkan satwa liar kehilangan tempat tinggal, berkembang biak, tempat bermain, dan sumber pakan salah satunya ialah burung. Penelitian ini bertujuan untuk monitoring keanekaragaman dan kepadatan populasi burung di Kawasan CSC LIPI sebagai dampak perkembangan suatu kawasan. Penelitian dilakukan pada Januari-Juli 2020 di Kawasan Hijau CSC-LIPI dengan menggunakan metode Point Count. Hasil penelitian ini tercatat sebanyak 40 spesies burung dengan kepadatan populasi tertinggi pada spesies Lonchura punctulata (7,18 ind/ha), Pycnonotus aurigaster (6,43 ind/ha), dan Collocalia linchi (2,76 ind/ha) dan kepadatan populasi terendah pada spesies Arachnothera longirostra, Artamus leucorhynchus, Eurystomus orientalis dengan nilai masing-masing sebesar 0,03 ind/ha. Pola persebaran spesies burung di Kawasan CSC-LIPI menunjukkan kategori mengelompok. Seiring berjalannya waktu, keragaman spesies maupun kepadatan populasi akan mengalami penurunan atau peningkatan sesuai dengan ketahanan adaptasi terhadap perubahan kondisi lingkungan dan persaingan dengan organisme lain.Abstract Birds are wild animals that play an important role in building the food chain, helping pollination, controlling insect pest populations, and seed dispersal agents that are useful for natural forest regeneration. An important role in the environment that makes birds an indicator of environmental quality. Cibinong Science Center (CSC) LIPI is an area that over time experiences changes in the landscape because there are new buildings that cause the green open area to decrease. This causes wild animals to lose their homes, breeds, places to play and sources of food, one of which is birds. This study aims to monitor the diversity and density of bird populations in the LIPI CSC area as a result of the development of an area. The study was conducted in January-July 2020 in the green house of CSC-LIPI area using the Point Count method. The results of this study recorded that 40 bird species with the highest population density were Lonchura punctulata (7.18 ind/ha), Pycnonotus aurigaster (6.43 ind/ha) and Collocalia linchi (2.76 ind/ha) and the density the lowest population was Arachnothera longirostra, Artamus leucorhynchus, Eurystomus orientalis with each value of 0.03 ind/ha. The distribution pattern of bird species in the CSC-LIPI area shows clustered categories. Species diversity and population density over time will decrease or increase in accordance with the resilience of adaptation to changes in environmental conditions and competition with other organisms.Keywords: Â Landscape ecology;Â Population density; Â Distribution patterns

Increasing Hermaphrodite Flowers using Plant Growth Regulators in Andromonoecious Jatropha curcas . DASUMIATI; . MIFTAHUDIN; . TRIADIATI; ALEX HARTANA; DIBYO PRONOWO
HAYATI Journal of Biosciences Vol. 21 No. 3 (2014): September 2014
Publisher : Bogor Agricultural University, Indonesia

Jatropha curcas (JC) is a crop with potential for use in biodiesel. Production of biodiesel requires plant seed as raw material, so the viability of JC for use in biodiesel will dependent greatly on the plant’s production of flowers. Generally, this plant is monoecious, meaning it has both male and female flowers. However, very rarely JC plants may be andromonoecious. Andromonoecious specimens of JC produce hermaphrodite and male flowers in the same plant. The number of hermaphrodite flowers per inflorescence is generally low compared to the number of male flowers. The aim of this study was to increase the proportion of hermaphrodite flowers by using plant growth regulators (PGRs) in andromonoecious JC. Our experiment was conducted in Randomized Block Design (RBD) with 9 treatments, namely kinetin, GA3, and IAA with concentrations of 0 ppm as a control, 50 and 100 ppm of each PGRs. The treatments were applied to stem cuttings from each plant and repeated 4 times. PGRs were applied by spraying the leaves within the buds of each plant. Applications took place weekly beginning when the plants entered flower initiating phase, until inflorescence produced. Observations were conducted during the treatment period (10 weeks). Results showed that plants treated with IAA, GA3, and kinetin at 50 and 100 ppm produced increased inflorescence per plant. The increases measured were 155.4 and 92.9% of (IAA), 120.4 and 151% (GA3), 96.6 and 51.7% (kinetin) respectively. In addition we found that application and GA3 at concentrations of 50 and 100 ppm, and kinetin at 50 ppm, increased the number of hermaphrodite flowers per inflorescence by 50%, and increased the number of hermaphrodite flowers per plant by 275.6 and 183.1% (IAA), 219.5 and 254.1% (GA3), 162.9 and 103.1% (kinetin) respectively. As would be expected, the number of fruit per plant increased in those specimens treated with IAA, GA3, and kinetin at 50 and 100 ppm. The increases measured were 301.7 and 167.4% (IAA), 211.7 and 257.0% (GA3), 162.5 and 101.4% (kinetin) respectively.

Androgynomonoecious Jatropha curcas: Chromosomes, Isozymes, and Flowers Gender Triadiati Triadiati; Kurniati Kurniati; Utut Widyastuti; Dasumiati Dasumiati
HAYATI Journal of Biosciences Vol. 26 No. 3 (2019): July 2019
Publisher : Bogor Agricultural University, Indonesia

Jatropha curcas (J. curcas) is usually monoecious plants, which have male and female flowers on the same inflorescence. However, J. curcas can be found as an androgynomonoecious plant (have male, female, and hermaphrodite flowers), even though very rare. Androgynomonoecious J. curcas can be identified after six months of planting when it had started flowering. Therefore, it is important to identify the characteristics of androgynomonoecious J. curcas that can differentiate between androgynomonoecious and monoecious plants in earlier stages of growth. The objectives of the research were to observe isozymes, chromosome and flowers gender of androgynomonoecious and monoecious J. curcas Banten and Lampung accessions. Seeds from five genotypes of J. curcas were used in the research. The observation was carried out on the chromosome and isozymes (Peroxidase and Esterase isozymes) could be used as markers to differentiate androgynomonoecious and monoecious plants. Observations about the flower gender from offsprings derived from different seeds were important to know the inheritance of flower gender. The androgynomonoecious and monoecious J. curcas were diploid with number of chromosomes 2n=2x=22. The chromosomes of androgynomonoecious have longer than that of monoecious J. curcas. The isozymes of androgynomonoecious J. curcas had four alleles and monoecious J. curcas (Banten female monoecious) had three alleles. The flower inflorescence and gender derived from androgynomonoecious plants were unstable, due to androgynomonoecious is intermediate state.

ISOLASI DAN AMPLIFIKASI DNA KELADI TIKUS (Thyponium flagelliform) UNTUK IDENTIFIKASI KERAGAMAN GENETIK Mohamad Fazri Hikmatyar; Juwartina Ida Royani; Dasumiati .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 2 (2015): December 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Keladi tikusÂ (Typhonium flagelliforme) is one of considerable potential medicinal plants, especially as anticancer herbal medicine. In Indonesia, this plant grows throughout the island of Java, in part of Kalimantan, Sumatra and Papua. The development of Keladi tikus plants to provide raw material to meet public demand is constrained with the quality of the plants that is not standardized yet. DNA marker technique has been widely used for identification of standardization and diversity of varieties. The aims of this research were to isolate DNA from 17 accessions of Keladi tikus from various regions in Indonesia and to amplify the DNA using ISSRÂ primers. The results obtained were 17 accessions of Keladi tikus that had been isolated using the modified CTAB method. Amplifications were done by using SBLT2 and SBLT8 primers that facilitated the appearance of the polymorphism bands on the 17 accessions of Keladi tikus. Thus, SBLT2 and SBLT8 primers can be used to identify genetic variations of Keladi Tikus.Keywords: Typonium flagelliforme, Keladi tikus, ISSR, medicinal plant, amplificationÂ ABSTRAKKeladi tikusÂ (Typonium flagelliforme) merupakan salah satu tanaman obatÂ yang cukup potensial khususnya sebagai obat herbal antikanker. Tanaman ini di Indonesia tersebar di sepanjang Pulau Jawa, sebagian Kalimantan, Sumatera dan Papua. Pengembangan tanaman keladi tikus untuk memenuhi bahan baku kebutuhan masyarakat saat ini terkendala pada mutu tanaman tersebut yang belum terstandar. Teknik penanda DNA telah banyak digunakan untuk standarisasi dan identifikasi keragaman varietas. Tujuan dari penelitian ini adalah untuk mengisolasi DNA dari 17 aksesi Keladi tikus dari berbagai daerah di Indonesia dan mengamplifikasi DNA tersebut dengan primer ISSR. Hasil penelitian menunjukkan bahwa 17 aksesi keladi tikus telah dapat diisolasi dengan menggunakan metode CTAB yang dimodifikasi. Amplifikasi dilakukan dengan primer SBLT2 dan SBLT8 yang mampu memunculkan pita-pita polimorfisme pada ke 17 aksesi Keladi tikus. Primer SBLT2 dan SBLT8 dapat digunakan untuk identifikasi variasi genetic Keladi tikus.Kata Kunci: Keladi tikus, Typonium flagelliforme, ISSR, tanaman obat, amplifikasi

Biosynthesis of ZnO Nanoparticles Using Pumpkin Peel Extract (Cucurbita moschata) and its Applications as Semiconductor in Dye Sensitized Solar Cell (DSSC) Nanda Saridewi; Dzikri Anfasa Firdaus; Isalmi Aziz; Biaunik Niski Kumila; Dasumiati Dasumiati
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 7, No. 2, November 2021
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v7i2.21046

ZnO nanoparticles are semiconductor materials that can be used in Dye Sensitized Solar Cells (DSSC). ZnO nanoparticles can be synthesized using pumpkin peel extract (Cucurbita moschata) which functions as a reducing agent, stabilizer, and capping agent. Zn(CH3COO)2.2H2O precursor was used with a concentration of 0.15 M at various pH 7, 8, and 9 reacted with pumpkin peel extract. The functional groups of pumpkin peel extract were characterized using Fourier Transform Infrared Spectroscopy (FTIR), the samples were analyzed by TEM and XRD. The resulting ZnO nanoparticles were used as semiconductors in Dye Sensitized Solar Cell (DSSC) using dyes from mangosteen peel.The FTIR results showed the presence of functional groups O-H hydroxy, CH2, secondary amides (R-CO-NR2, C-H and phosphate (PO43-). XRD results showed that ZnO produced wurzhite crystals with a hexagonal system and the smallest crystal size was 18.99 nm. TEM results showed that ZnO synthesized at a concentration of 0.15 M and pH 8 had a spherical particle shape with a size of 24.90 nm, while the DSSC test results had an efficiency of 9.06 x 10-4%.

Keragaan Malai Mutan Padi Generasi M1 Hasil Iradiasi Gamma Sherly Rahayu; Via Destavany; Dasumiati Dasumiati
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 16, No 2 (2020): Desember 2020
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/jair.2020.16.2.5763

Pemanfaatan iptek nuklir dalam pemuliaan tanaman di Indonesia telah banyak dirasakan dengan telah dihasilkan beberapa varietas padi dalam kurun waktu empat dekade terakhir. Perubahan keragaan tanaman padi pada generasi awal merupakan akibat dari pengaruh radiasi yang bersifat acak terhadap genetik tanaman. Tujuan penelitian ini adalah untuk mempelajari keragaan karakter agronomi, khususnya morfologi malai mutan padi pada generasi M1 hasil mutasi induksi pada dosis optimum 200 dan 300 Gy. Penelitian dilakukan menggunakan Rancangan Acak Kelompok Lengkap dengan 3 ulangan, mengunakan jarak tanam 20 cm x 20 cm dengan satu bibit per lubang. Dosis radiasi yang digunakan yaitu 0, 200, dan 300 Gy, menggunakan dua varietas padi. Hasil penelitian menunjukkan bahwa karakter malai seperti jumlah malai, jumlah cabang primer, jumlah cabang sekunder, dan jumlah gabah isi mengalami penurunan seiring meningkatnya dosis iradiasi hingga 300 Gy. Hal ini berbanding terbalik dengan karakter jumlah gabah hampa yang semakin meningkat sebagai respon terhadap dosis iradiasi, sedangkan karakter panjang malai tidak mengalami perubahan berarti akibat perlakukan iradiasi gamma pada generasi M1.

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