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All Journal Jurnal Ilmiah Kedokteran Wijaya Kusuma Jurnal Medika Malahayati
Sariwidyantry, Raden Ghita
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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EKSTRAK METANOL TERIPANG (Holothuria scabra) MENURUNKAN EKSPRESI GEN COX-2 PADA HEPAR MENCIT (Mus musculus) BETINA GALUR C57BL YANG DIINDUKSI DMBA DAN HIGH FAT DIET Sariwidyantry, Raden Ghita; Maratantiningtyas, Demes Chornelia; Ratnawati, Hana; Liliana, Teresa; Sanjaya, Ardo
Jurnal Medika Malahayati Vol 7, No 4 (2023): Volume 7 Nomor 4
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jmm.v7i4.11277

Abstract

Abstrak : Ekstrak Metanol Teripang (Holothuria scabra) Menurunkan Ekspresi Gen Cox-2 Pada Hepar Mencit (Mus musculus) Betina Galur C57BL Yang Diinduksi DMBA dan High Fat Diet. Saat ini NAFLD banyak dikenal sebagai penyebab utama penyakit hepar kronis di Asia, dimana diperkirakan sepertiga populasi negara berkembang memiliki bukti adanya steatosis dengan mayoritas berada pada kondisi simple steatosis (70-90%). Sama seperti penyakit hepar kronis pada umumnya, NAFLD juga didasari oleh mekanisme stres oksidatif yang melibatkan mediator inflamasi seperti TNF-α, IL-6, tromboksan, dan prostaglandin yang diketahui mampu meregulasi reaksi inflamasi dan pertumbuhan kanker pada kolon dan hepar. Dalam penelitian ini digunakan 30 ekor mencit betina galur C57BL yang diinduksi DMBA dan diberi pakan tinggi lemak, dibagi dalam 2 kelompok kontrol dan 3 kelompok perlakuan yang diberi ekstrak metanol teripang dengan dosis 0,33 g/KgBB ; 0,66 g/KgBB ; 0,99 g/KgBB selama 6 minggu. Mencit dikorbankan, kemudian organ hepar diekstraksi dan dilakukan pemeriksaan ekspresi gen COX-2 dengan metode semikuantitatif RT-PCR. Hasil penelitian diuji secara statistik menggunakan ANOVA dan Post Hoc Test Tukey HSD yang menunjukkan hasil signifikan dengan nilai P<0,001. Ekspresi gen COX-2 berbanding terbalik dengan konsentrasi ekstrak metanol teripang. Hal ini menunjukkan bahwa ekstrak metanol teripang memiliki efek antiinflamasi melalui cara menurunkan ekspresi gen COX-2.
Modulated Hepatic Expression of NF-kB and CCN1 Genes in a Breast Cancer Mouse Model Induced by DMBA and High-Fat Diet Martantiningtyas, Demes Chornelia; Sariwidyantry, Raden Ghita; Ratnawati, Hana; Wargasetya, Teresa Lilliana; Sanjaya, Ardo
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol. 14 No. 1 (2025): MARET 2025
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jikw.v14i1.4002

Abstract

Background: The study investigates the combined effects of 7,12-dimethylbenz[a]anthracene (DMBA) and a high-fat diet (HFD) on hepatic gene expression in a breast cancer mouse model. Both DMBA and HFD are associated with oxidative stress, inflammation, and fibrosis, which may contribute to cancer progression.Methods: Female C57BL/6 mice were divided into three groups: standard diet (ND), ND + DMBA, and HFD + DMBA. Mice in the ND + DMBA and HFD + DMBA groups received DMBA injections weekly for six weeks. The HFD + DMBA group was also fed a high-fat diet for four weeks prior to DMBA administration. After a 12-week treatment period, liver tissues were collected and analyzed for NF-κB and CCN1/CYR61 gene expression using semi-quantitative RT-PCR.Results: The study revealed significant upregulation of NF-κB and CCN1/CYR61 expression in the ND + DMBA and HFD + DMBA groups compared to the control group. Notably, the HFD + DMBA group exhibited the highest expression levels of these genes, suggesting that the combination of HFD and DMBA synergistically exacerbates hepatic inflammation and fibrosis.Conclusion: The findings indicate that a high-fat diet in combination with carcinogen exposure can significantly enhance hepatic gene expression related to inflammation and fibrosis, highlighting the importance of dietary management in cancer prevention and treatment. 
Co-Authors Ardo Sanjaya Demes Chornelia Martantiningtyas, Demes Chornelia HANA RATNAWATI Liliana, Teresa Maratantiningtyas, Demes Chornelia Wargasetya, Teresa Lilliana