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Sabolakna, Asep
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Analisis Perbedaan Variasi Derajat Keasaman terhadap Tingkat Spesifisitas Metode Kastle-Meyer dalam Pendeteksian Darah Sabolakna, Asep; Angelia, Kimberly
Jurnal Kesehatan Vol 13 No 2 (2022): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v13i2.3038

Abstract

Blood is evidence of a crime that can be damaged by humans and environmental factors. One of the contaminants that have the potential to affect the specificity of the Kastle-Meyer method in detecting blood is the variation in the degree of acidity (pH). Specificity analysis of the Kastle-Meyer method is needed to determine the ability of the Kastle-Meyer method to detect blood in the presence of other components/contaminants that may be present in a sample. The purpose of this study was to analyze the difference in the addition of an acidic solution with a pH of 1.2-8.4 on the specificity of blood detection using the Kastle-Meyer method. The research was conducted in the form of pre-experimental research with a static-group comparison design. The sampling technique used was purposive sampling. Blood samples that have been added to an acid solution with a certain pH (pH 1,2; 2.4; 3.6; 4.8; 6; and 7.2) were then tested using the Kastle-Meyer method. The principle of the Kastle-Meyer method is based on the activity of the peroxidase enzyme present in hemoglobin in the blood. The results showed the specificity of the Kastle-Meyer method on blood samples with acid solution contaminants with a pH of 1.2; 2,4; 3,6; 4.8; 6.0; 7.2; and 8.4 respectively at 70%, 70%, 90%, 60%, 60%, 90%, 80%. The results of data analysis from the Kruskal-Wallis test showed a significance p-value 0.269 > 0.05, so it can be concluded that there is no difference in the specificity of the Kastle-Meyer method in detecting blood in the addition of acid solutions with different acidity degrees.
Kualitas Hasil Uji Limbah Pool-Plasma Komponen Packed Red-Blood Cell (PRC) Sebagai Antisera Golongan Darah ABO Wahdaniah, Wahdaniah; Margini, Fitria; Sabolakna, Asep
Jurnal Laboratorium Khatulistiwa Vol 5, No 2 (2022): Mei 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v5i2.975

Abstract

PRC is the most needed component for current blood transfusion. Plasma is one of the separation product of PRC from whole blood. These components are often discarded due to low plasma transfusion requirements. The residual plasma contains natural ABO agglutinins that can be utilized as polyclonal antisera in blood grouping tests. This study aims to determine the quality of pool residual plasma of PRC component as the ABO blood grouping antisera. This research uses descriptive analytic design in the form of comparative study. Samples of the test materials and the testers were obtained by purposive sampling, which consisting of each 20 units of A, B and O (positive control) plasma, and also 2 units of AB plasma (negative control) as the test materials, and each 25 of A and B fresh blood specimens as the tester materials. All of those materials should meet the inclusion and exclusion criteria of this study. According to their blood type, each of the plasma were mixed proportionaly according to its weight. Then, each pool-plasma and the commercial antisera were tested together against the same fresh blood specimens by the slide method. The degree of each agglutination was observed by 2 panelists, and then analyzed statistically by Wilcoxon Signed Rank Test with error rate (α) = 5%. The degree of agglutination by using the B pool-plasma against the A fresh blood was obtained in proportions of 8.0% (2+), 38.0% (3+) and 54.0% (4+) with the difference in proportion of 34.0% (3+) higher and 42.0% (4+) lower than anti-A commercial antisera. Meanwhile, by using the A pool-plasma against the B fresh blood was obtained in proportion of 18,0% (3+) and 82,0% (4+) with difference in proportion of 18,0% (3+) higher and 18,0% (4+) lower than anti-B commercial antisera. There are significant differences between the commercial antisera and the pool residual plasma as blood grouping test materials based on observation of all panelists (p value = 0.000).
Co-Authors Angelia, Kimberly Margini, Fitria Wahdaniah Wahyudi