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Ihsan, Bagus Muhammad
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PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION Ihsan, Bagus Muhammad; Sucipto, Cecep Dani; Khayan, Khayan
Journal of Vocational Health Studies Vol. 5 No. 1 (2021): July 2021 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V5.I1.2021.26-30

Abstract

Background: Filariasis patients can be a source of transmission if their blood still contains microfilariae. One of the Polymerase Chain Reaction (PCR) methods used is High Resolution Melting (HRM), using primary specificity testing. Purpose: To test the specificity of SLX primer. The samples used for this test were isolates of Salmonella., Klebsiella, Pseudomonas, negative and positive controls for Brugia malayi and Wuchereria bancrofti. Method: The design in this study is a quasi-experiment by testing the specificity of SLX primer using HRMbased real-time PCR based on the Cycle Threshold (CT) value observed through the amplification curve. Result: The real-time PCR results showed that no CT was released in the bacterial samples, and there was a CT value in the positive control. The results of this study indicate that specific SLX primer can be used in identifying microfilariae. Conclusion: SLX primer have a reasonable specificity because they cannot detect the existence of microorganisms in the samples other than microfilariae.
IDENTIFICATION OF MICROFILARIAE USING CONVENTIONAL POLYMERASE CHAIN REACTION AND QPCR-HRM Ihsan, Bagus Muhammad; Lakshmi Puspita, Widyana; Triana, Linda; Wahdaniah; Khayan; Dani Sucipto, Cecep
Journal of Vocational Health Studies Vol. 8 No. 1 (2024): July 2024 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V8.I1.2024.42-47

Abstract

Background: The presence of filarial worms in the lymph nodes can result in acute symptoms, such as inflammation of the lymph nodes and ducts, particularly in the groin region. As part of the life cycle of filariasis, symptomatic or asymptomatic patients with microfilariae in their blood can transmit the disease via mosquito bites. The inspection of microfilariae that is currently being developed uses Polymerase Chain Reaction (PCR) to carry out a unique DNA search technique. Purpose: Identify the type of microfilaria present in filariasis patients using Quantitative PCR High- Resolution Melting (qPCR-HRM) and conventional PCR techniques. Method: This study involved the examination of 19 samples using the qPCR-HRM method. Subsequently, the results that were considered positive for microfilaria underwent further testing using conventional PCR. Result: The results of the examination using these two methods revealed the presence of Brugia malayi and Wuchereria bancrofti microfilariae with peak melting temperatures ranging from 78.2 – 78.7 °C and 80.8 – 81.2 °C, and fragment sizes of 199 bp and 227 bp, respectively. Conclusion: Based on the results of the identification from these two methods, it is evident that microfilariae of Brugia malayi and Wuchereria bancrofti can be detected using both conventional and qPCR-HRM methods.
UJI EKSTRAK ETANOL DAUN SRIKAYA (ANNONA SQUAMOSA) TERHADAP DAYA HAMBAT BAKTERI SHIGELLA DYSENTERIAE Supriyanto, Supriyanto; Firmanlindo, Dimas Satria; Ihsan, Bagus Muhammad; Slamet, Slamet
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 16 No 2 (2024): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v16i2.2558

Abstract

Daun srikaya memiliki kandungan senyawa kimia flavonoid, saponin, alkaloid, tanin dan fenolik yang dapat digunakan untuk menghambat pertumbuhan bakteri Shigella dysentriae penyebab diare disentri. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak etanol daun srikaya (Annona squamosa) terhadap bakteri Shigella dysenteriae dengan mengukur zona hambat dengan konsentrasi 65%, 70%, 75%, dan 80% terhadap bakteri Shigella dysentriae pada media Muler Hinton Agar. Desain penelitian eksperimen semu dengan menggunakan uji deskriptif. Metode penelitian ini adalah metode difusi agar dengan mengukur zona hambat shigella dysentriae pada media MHA Kirby Bauer. Setelah didapatkan zona hambat dilakukan analisis efektivitas ekstrak etanol daun Srikaya (Annona squamosa) konsentrasi 65%, 70%, 75% dan 80% terhadap bakteri Shigella dysentriae pada media Muler Hinton Agar. Dari hasil penelitian didapatkan nilai rata-rata (mean) zona hambat konsetrasi 65% sebesar 7,91 mm, konsentrasi 70% sebesar 9,41 mm, konsentrasi 75% sebesar 12,50 mm dan konsenrasi 80% sebesar 13,25 mm, sehingga rata-rata zona hambat yang terbentuk pada konsentrasi 65% dan 70% masuk ke dalam kategori resisten, kemudian rata-rata zona hambat yang terbentuk pada konsentrasi 75% dan 80% masuk ke dalam kategori intermediet terhadap bakteri Shigella dysentriae jika dibandingkan dengan Tetracycline. Kesimpulan pada penelitian ini adalah ekstrak daun srikaya tidak efektif dalam menghambat bakteri Shigella dysentriae, karena tidak memiliki kemampuan kategori sensitif yang sama dengan Tetracycline
ANALISIS ANTI BAKTERI SEDIAAN GEL PEMBERSIH TANGAN EKSTRAK ETANOL DAUN BELIMBING WULUH TERHADAP BAKTERI Escherichia coli Sya’baniar, Luthfiranda; Suwandi, Edy; Ihsan, Bagus Muhammad; Sari, Emilda; Fatayati, Imma
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1492

Abstract

Antiseptik digunakan untuk membunuh atau menghambat pertumbuhan mikroorganisme pada jaringan hidup. Daun belimbing wuluh mengandung senyawa metabolit antibakteri seperti alkaloid, flavonoid, saponin, tanin, fenol, steroid dan triterpenoid. Tujuan penelitian ini menjelaskan perbedaan gel pembersih tangan ekstrak daun belimbing wuluh dua macam formula dalam menghambat Escherichia coli. Dengan purposive sampling, sampel yang digunakan adalah ekstrak daun belimbing wuluh dengan 2 perlakuan yaitu kosentrasi 7,5% dan 15% yang masing-masing dilakukan pengulangan sebanyak 16 kali dengan metode difusi cakram. Hasil penelitian yaitu sediaan gel pembersih tangan memiliki aktivitas antibakteri terhadap Escherichia coli pada formula I memiliki zona hambat rata-rata 7,4mm dan formula II memiliki zona hambat rata-rata 9,6mm kategori sedang. Hasil organoleptis warna hijau bau khas berbentuk semi solid dan rata-rata pH 6. Hasil uji Wilcoxon didapatkan p value 0,000 < α 0,05 artinya terdapat perbedaan gel pembersih tangan ekstrak daun belimbing wuluh formula I dan formula II dalam mengahambat pertumbuhan Escherichia coli.
EFEKTIVITAS SEDUHAN DAUN ASAM JAWA TERHADAP KADAR HOMA-IR DAN HOMA-β PADA PASIEN DIABETES MELLITUS TIPE 2 Nopriantini, Nopriantini; Purba, Jonni Syah R; Desi, Desi; Petrika, Yanuarti; Novia, Devi; Ihsan, Bagus Muhammad
Media Penelitian dan Pengembangan Kesehatan Vol. 34 No. 3 (2024): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v34i3.2014

Abstract

This study assessed the impact of tamarind leaf tea on HOMA-IR and HOMA-β levels in patients with Type 2 Diabetes Mellitus (DM). Thirty-two patients aged 20-50 years from Sungai Ambawang District Health Center, who were not using any DM medication, participated. They were divided into control (KN) and treatment (KP) groups, with KP receiving a tamarind leaf tea infusion for 7 days. HOMA-IR and HOMA-β levels were calculated using standardized formulas based on fasting insulin and glucose levels. The Wilcoxon signed-rank test revealed a significant decrease in HOMA-IR levels in the KP group (p<0.05), while no significant change was observed in the KN group. Although no significant difference in HOMA-β levels was found between the two groups, the KP group showed a notable increase by 4. These findings suggest that a 7-day tamarind leaf tea infusion effectively reduced HOMA-IR levels in patients with type 2 DM, although it did not significantly affect HOMA-β levels. This study highlights the potential of tamarind leaf tea as a complementary approach for managing insulin resistance in Type 2 DM patients. Further research is needed to explore the long-term effects and mechanisms of action for improving β-cell function. The conclusion of this study is that steeping tamarind leaves for seven days can reduce HOMA-I levels and slightly increase HOMA-β levels in patients with type 2 Diabetes Mellitus.
OPTIMAL COMBINATION OF TOLUENE AND CLOVE OIL (Syizigium aromaticum) IN THE PROCESS OF CLEARING THE PERMANENT PREPARATION PEDICULUS HUMANUS CAPITIS Ihsan, Bagus Muhammad; Liesvia, Vira; Armal, Hadits Lissentiya; Supriyanto, Supriyanto; Hanif, Muhammad Ifham
Media Penelitian dan Pengembangan Kesehatan Vol. 34 No. 2 (2024): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v34i2.2006

Abstract

Clearing is a vital process in microscopy, aimed at enhancing the transparency of tissue preparations for clearer observation of insect structures. Despite its effectiveness, Toluene poses safety concerns due to its explosive nature. Therefore, clove oil (Syzygium aromaticum) has been explored as a safer alternative. This study evaluates the quality of permanent preparations of Pediculus humanus capitis using both toluene and clove oil. Comparative tests were conducted, treating adult head lice specimens with each solvent for three repetitions. Permanent preparations were assessed for clarity, color quality, and morphological integrity. Results indicate a significant difference in quality between the two methods. Toluene yielded mainly poor-quality preparations, while clove oil resulted in a higher proportion of preparations of good quality. The Mann-Whitney test confirmed the significance of this difference (p = 0.002). This study highlights the efficacy of clove oil as a safer alternative to toluene in the clearing process, contributing to entomological practices and chemical safety.
Aplikasi teknologi tepat guna pengolahan air sebagai sumber air bersih masyarakat Khayan; Ihsan, Bagus Muhammad; Sucipto, Cecep Dani; Puspita, Widyana Lakshmi
Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Vol. 4 No. 2 (2023)
Publisher : Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jp2m.v4i2.20460

Abstract

Gangguan kesehatan di Indonesia yang berhubungan dengan penyakit yang ditularkan melalui air (Water Borne Disease) masih tergolong tinggi, seperti Diare dan hepatitis. Timbulnya penyakit menular terkait Water Borne Disease tersebut, diantaranya karena tidak terpenuhinya kuantitas dan kualitas air baku sebagai sumber air minum yang memenuhi syarat kesehatan. Metode pengabdian masyarakat ini dilakukan beberapa metode, yaitu pendekatan masyarakat, penyuluhan dan pembuatan sarana Pengolahan Air Permukaan dengan Teknologi Tepat Guna (TTG) kombinasi, yaitu dengan Aerasi, Filtrasi Pasir dan Absorpsi Kabon Aktif untuk Menurunkan kandungan Fe, Kekeruhan, Warna dan pH Air permukaan sebagai sumber air minum masyarakat di Desa Pranan Kota Serang. Hasil dan kesimpulan dari pengabdian pada masyarakat ini adalah Terdapat peningkatan pengetahuan masyarakat tentang TTG Filtrasi Air Bersih , Terciptanya revitalisasi sumber air bersih, transfer teknologi serta dampak sosial bagi masyarakat yaitu perubahan pola kegiatan mandi dan mencuci yang sebelumnya dilakukan di kali pembuangan air sawah setelah pelaksanaan pengabdian kegiatan tersebut beralih ke rumah masing-masing dan Metode penjernihan air secara filtrasi terbukti dapat meningkatkan kualitas air sumur bor ,sehingga air berada pada ambang batas layak digunakan untuk kehidupan sehari-hari. Dengan ini masyarakat setempat tidak lagi mengambil air dari sungai pembuangan air sawah.
Identification of Pathogenic Bacteria in Tofu Water and Sugarcane Water in Pontianak City Hermantika, David Deovia Triputra; Ihsan, Bagus Muhammad; Kamilla, Laila; Khayan, Khayan; Hanif, Muhammad Ifham
Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 4 (2024): February
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v17i4.3336

Abstract

Tofu water and sugar cane juice are trendy drinks, especially in Pontianak City. Pathogenic bacterial contamination in tofu water and sugar cane juice can be a severe threat if the immune system is weak. This research aims to identify pathogenic bacteria in Pontianak City tofu water and sugar cane water. The methods used are culture methods and biochemical tests. The research samples were tofu water and sugar cane juice sold in the community. The sample was selected using a purposive sampling technique. In this study, the bacteria found in the identification of bacteria in tofu water and sugar cane water were Klebsiella pneumoniae, Shigella dysenteriae, Staphylococcus aureus, Enterobacter aerogenes, Salmonella typhi, and Escherichia coli. Bacteria dominate, while in sugarcane juice, it is dominated by Escherichia coli, followed by Klebsiella pneumoniae. The research results can also be the basis for better policies and preventive measures related to the safety of the quality of tofu water and sugar cane juice in Pontianak City. Therefore, this research can be relevant to environmental protection and public health in Pontianak.
Gambaran Kadar Glukosa Darah Sewaktu Pada Ibu Hamil di Puskesmas Tambelan Sampit Kota Pontianak Ramadan, Aditiya; Nuswantoro, Ari; Triana, Linda; Ihsan, Bagus Muhammad
Jurnal Ners Vol. 8 No. 1 (2024): APRIL 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v8i1.22203

Abstract

characterised by high blood sugar levels as well as problems with protein, lipid, and carbohydrate metabolism as a result of inadequate insulin activity. Patients who have wounds infected by pathogenic bacteria, both Gram positive and Gram negative, run the risk of developing a number of problems, including neuropathy, hypertension, coronary heart disease, retinopathy, nephropathy, and gangrene. This cross-sectional, observational, descriptive study aims to pinpoint pathogenic bacteria in the diabetic patients' wounds at the diabetes wound care clinic in Pontianak City. The bacterial identification stage includes inoculation on enrichment media, Gram staining, inoculation and observation of colonies on differential-selective media, and biochemical tests. Examination results showed that Klebsiella pneumoniae was identified in 20 samples (57%), Pseudomonas aeruginosa in 8 samples (23%), and Staphylococcus aureus in 7 samples (20%). According to the study, diabetic wound problems put DM patients at risk of contracting dangerous microorganisms and spreading them to others. In order to prevent infection and other health issues, patients are instructed on how to properly care for their wounds. They are also provided the correct follow-up by medical professionals.
Efektivitas Ekstrak Batang Brotowali (Tinospora Crispa) sebagai Larvasida Terhadap Culex Sp Sutriswanto, Sutriswanto; Ihsan, Bagus Muhammad; Cahyani, Mely
MAHESA : Malahayati Health Student Journal Vol 4, No 7 (2024): Volume 4 Nomor 7 (2024)
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mahesa.v4i7.14624

Abstract

ABSTRACT Filariasis (elephantiasis) is a disease caused by the Culex quinquefasciatus mosquito vector. Eradicating larvae is one way of controlling the Culex mosquito vector. Brotowali stems (Tinospora crispa) have potential as a natural larvicide. The contents of brotowali (Tinospora crispa) stem extract include alkaloids, tannins, saponins, glycosides, terpenoids and flavonoids. This research aims to determine the effectiveness of brotowali (Tinospora crispa) stem extract as a larvicide against Culex sp. The research method used was laboratory experimental using 4 variations of concentration, namely 4%, 6%, 8% and 10% with 5 treatments using Culex sp larvae. Based on research results, the lowest death concentration level is 4% with a death percentage of 48% and the highest death concentration level is 10% with a death percentage of 82%. In the One Way ANOVA test, the p value = 0.000, where the p value < 0.05, means that brotowali (Tinospora crispa) stem extract is effective as a larvicide for Culex sp mosquitoes. Keywords: Culex sp, Bitter vine (Tinospora crispa) , Extract ABSTRAK Penyakit Filariasis (kaki gajah) merupakan salah satu penyakit yang disebabkan oleh vektor nyamuk Culex quinquefasciatus. Pemberantasan larva merupakan salah satu pengendalian vektor nyamuk Culex. Batang brotowali (Tinospora crispa) memiliki potensi sebagai larvasida alami. Kandungan ekstrak batang brotowali (Tinospora crispa) antara lain Alkaloid, Tanin, Saponin, Glikosida, terpenoid dan flavonoid. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak batang brotowali (Tinospora crispa) sebagai larvasida terhadap Culex sp. Metode penelitian yang digunakan adalah eksperimental laboratorium dengan menggunakan 4 variasi konsentrasi yaitu 4%, 6%, 8% dan 10% dengan 5 kali perlakuan menggunakan larva Culex sp. Berdasarkan hasil penelitian tingkat konsentrasi kematian terendah yaitu 4% dengan persentase kematian 48% dan tingkat konsentrasi kematian tertinggi yaitu 10% dengan persentase kematian 82%. Pada uji One Way ANOVA diperoleh nilai p value = 0.000 dimana nilai p value < 0.05 hal ini dapat diartikan bahwa Ekstrak batang brotowali (Tinospora crispa) efektif sebagai larvasida nyamuk Culex sp Kata Kunci: Culex sp, Batang Brotowali, Ekstrak
Co-Authors Aditia, Aditia Affifatuzahara, Affifatuzahara Al-qorifah, Fahma Ajeng Ana, Novika Dewi Anjelica, Benedikta Ari Nuswantoro Ari Widiyantoro Armal, Hadits Lissentiya Barlian, Barlian Cahyani, Mely Cecep Dani Sucipto Christian , Diky Desi Desi, Desi Djohan, Herlinda Firmanlindo, Dimas Satria Hanif, Muhammad Ifham Hermantika, David Deovia Triputra Imma Fatayati Indrawati, Ratih Jonni Syah R. Purba Khayan Khayan Laila Kamilla Liesvia, Vira Linda Triana Maulidiyah Salim Nasihin, Nasihin Nopriantini Novia, Devi Ramadan, Aditiya Ramadhanti, Bella Ratnawaty, Gervacia Jenny Sabolakna, Asep Sari, Emilda Shafitri, Anisya Nur Slamet . Supriyanto Supriyanto Sutriswanto, Sutriswanto Suwandi, Edy Sya’baniar, Luthfiranda Tjamin, Yeremiah Rubin Uq'ma, Nurul Wahdaniah Widyana Lakshmi Puspita Yanuarti Petrika