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All Journal PELS (Procedia of Engineering and Life Science) Journal of Medical and Health Science
Hamad, Sara Salam
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Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Electrical & Electronics Engineering Engineering Health Professions Medicine & Pharmacology Public Health

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Effective Primer Selection for Differentiating Periplaneta Species via PCR Khaled, Shifaa Waleed; Hamad, Sara Salam
Procedia of Engineering and Life Science Vol. 4 (2023): Proceedings of the 6th Seminar Nasional Sains 2023
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/pels.v4i0.1630

Abstract

This study utilized random polymerase chain reaction (PCR) with 10 random primers to differentiate between two insect species, Aegyptica Periplaneta and Periplaneta japonic. The results revealed a total of 29 bands, with 23 being polymorphic. The classification of the animal kingdom, especially insects, is complex due to the abundance and diversity of organisms. Traditional phenotypic methods often fall short in identifying closely related species, necessitating molecular approaches. Primers OP-A04, OP-A08, OP-B14, OP-B18, OP-C10, OP-C15, and OP-C18 showed the highest polymorphism (100%), while OP-A15 and OP-B09 had the lowest (40%). Primer OP-C06 exhibited no bands, indicating 0% polymorphism. Primers OP-A08 and OP-A15 had the highest efficiency (17.241%), whereas OP-C06, OP-C10, and OP-C15 had the lowest (3.448%). Notably, primer OP-A15 successfully discriminated between the species, while OP-C06 failed entirely. These findings underscore the importance of primer selection in molecular identification and suggest OP-A15 as a reliable primer for distinguishing between these Periplaneta species. Highlights: Primer OP-A15 effectively discriminated between Aegyptica Periplaneta and Periplaneta japonic. High polymorphism observed with primers OP-A04, OP-A08, OP-B14, OP-B18, OP-C10, OP-C15, and OP-C18. Primer OP-C06 showed 0% polymorphism and failed to differentiate between species. Keywords: Insect Classification, PCR, Random Primers, Species Differentiation, Periplaneta
Study of the Efficacy of Colistin against Wound Contamination by Acinetobacter baumannii Hamad, Sara Salam; Hussein, Arwa Alaa; Razzaq, Alaa Siham Abdul
JOURNAL OF MEDICAL AND HEALTH SCIENCE Vol. 3 No. 1 (2025): Juli
Publisher : Universitas Muhammadiyah Sidaorjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/anamnetic.v3i1.1608

Abstract

Background.Acinetobacter baumannii is a multidrug-resistant (MDR) Gram-negative pathogen commonly associated with wound infections, particularly in hospital and battlefield settings. Due to its resistance to multiple antibiotic classes, treatment options are limited. Colistin (polymyxin E), a last-resort antibiotic, has been reintroduced for treating MDR A. baumannii infections. Objective: This study aimed to evaluate the efficacy of colistin against A. baumannii isolated from wound contamination cases. Methods: A total of (30) wound swabs were collected from patients with clinical signs of infection. Isolation and identification of (10) isolations of A. baumannii and (20) were different species. A. baumannii conducted using standard microbiological and biochemical methods, followed by confirmation via Vitek 2 compact. Antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer disk diffusion method, and colistin minimum inhibitory concentration (MIC) was determined by broth microdilution following CLSI/EUCAST guidelines. Results: According to this study on Acinetobacter baumannii, the bacteria might stop the bacteria's development at various colistin concentrations. In contrast to 500 µg/ml, the higher concentration (1000 µg/ml) generated a wider zone of inhibition, indicating dose-dependent antibacterial action. A wider zone of inhibition was seen at the higher concentration, suggesting dose-dependent antibacterial action. The study also discovered that following skin damage, skin cells progressively restored to their original architecture, however inflammatory cells and cellular debris persisted. Significant necrosis was seen in the second group, suggesting a serious inflammatory reaction. The third group displayed epidermal cell regeneration, with the group that received 1000 µg/ml of colistin exhibiting more noticeable regeneration. Conclusion: Colistin remains a highly effective antibiotic against MDR A. baumannii in wound infections. However, its use should be monitored due to the risk of emerging resistance and potential nephrotoxicity. Regular surveillance and antibiotic stewardship programs are essential to preserve their efficacy
Co-Authors Hussein, Arwa Alaa Khaled, Shifaa Waleed Razzaq, Alaa Siham Abdul